Isolation and identification of pathogen of anthracnose of lily in Hunan Province

doi:10.14088/j.cnki.issn0439-8114.2025.10.015

Abstract

Abstract: The pathogen of lily(Lilium brownii var. viridulum Baker) anthracnose in the main production areas of Hunan Province was isolated and purified using the traditional tissue block separation method. Through a combination of morphological and molecular biological methods, it was systematically identified. The results indicated that the primary pathogenic fungus was Colletotrichum spaethianum. The pathogenicity of the isolated strains was confirmed by pathogenicity tests. Additionally, differences in the infection by this pathogen between two cultivated lily varieties in Hunan Province were compared and analyzed.

Key words: lily (Lilium brownii var. viridulum Baker), Colletotrichum spaethianum, isolation and identification, cluster analysis

CLC Number:

DENG Qiao, CHU Chang-bin, FANG Dong-zhen, CAI Dao-hui, GAO Chun-sheng. Isolation and identification of pathogen of anthracnose of lily in Hunan Province[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 99-104.

References

[1] 杜方,李兴桃,徐小晶,等.百合品种的分类及遗传多样性研究[J].山西农业大学学报(自然科学版),2018,38(5):16-22.
[2] 刘红超,宋涛,张朝春.食用百合产量影响因素的整合分析[J].江苏农业科学,2021,49(12):90-94.
[3] 田雪慧,刘秀云,任艳芬.食用百合研究进展及展望[J].西北园艺:综合,2020(5):24-26.
[4] LI Y E,WANG H H,ZHANG W B,et al.Evaluation of nutrition components in Lanzhou lily bulb by confocal Raman microscopy[J].Spectrochimica acta part A: Molecular and biomolecular spectroscopy,2021,244:118837.
[5] KONG Y, WANG H, LANG L X, et al.Metabolome-based discrimination analysis of five Lilium bulbs associated with differences in secondary metabolites[J].Molecules,2021,26(5):1340.
[6] 孙佳宁,连希希,孙伶俐,等.百合主要成分及药理作用研究进展[J].中国野生植物资源,2022,41(7):45-50.
[7] TANG W P, MUNAFO J P Jr,PALATINI K,et al. Hepatoprotective activity of Easter lily (Lilium longiflorum Thunb.) bulb extracts[J].Journal of agricultural and food chemistry,2015,63(44):9722-9728.
[8] CHI X S,WANG S J,BALOCH Z,et al.Research progress on classical traditional Chinese medicine formula Lily Bulb and Rehmannia decoction in the treatment of depression[J].Biomedicine & pharmacotherapy,2019,112: 108616.
[9] 赵银彦,李正录,蔡林海.中国食用百合生产现状与发展思路[J].甘肃科技,2018,34(8):1-2,52.
[10] 刘彤珂,宋水林,熊桂红,等.百合根腐病病原分离与鉴定[J].生物灾害科学,2023,46(1):1-7.
[11] 陈军华,吴剑锋,方洁,等.百合连作障碍形成机制及防治技术研究进展[J].长江蔬菜,2019(20):41-47.
[12] SHI G Y,SUN H Q,CALDERÓN-URREA A,et al. Soil fungal diversity loss and appearance of specific fungal pathogenic communities associated with the consecutive replant problem (CRP) in lily[J].Frontiers in microbiology,2020,11:1649.
[13] 仇硕,夏科,李秀娟,等.龙牙百合两种病害致病菌的分离、鉴定及抑制效应研究[J].广西植物,2018,38(1):109-118.
[14] CHAI N,XU J,ZUO R M,et al.Metabolic and transcriptomic profiling of Lilium leaves infected with Botrytis elliptica reveals different stages of plant defense mechanisms[J].Frontiers in plant science,2021,12:730620.
[15] 吴金平,丁自立,刘晓艳,等.卷丹百合黑斑病病原鉴定及生物学特性研究(英文)[J].Agricultural science & technology,2014,15(11):1879-1882.
[16] 唐祥宁,游春平,刘福秀,等.江西百合病害调查与鉴定[J].江西农业学报,1997,9(4):1-8.
[17] 程维舜,祝菊红,蔡翔,等.百合炭疽病研究进展[J].中国植保导刊,2020,40(2):33-36,25.
[18] 曾慧兰,李润根,卢其能.龙牙百合炭疽病病原菌鉴定[J].植物病理学报,2020,50(6):779-783.
[19] 吕昭龙,李继平,郑果,等.苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定[J].植物医学,2023,2(1):34-43.
[20] 吴峰婧琳,陈健鑫,王芳,等.云南油茶炭疽病病原鉴定及发病规律研究[J].西北师范大学学报(自然科学版),2023,59(2):118-127.
[21] 王绪霞,汤涤洛,彭洪翠,等.苎麻炭疽病病原菌的分离与鉴定[J].中国麻业科学,2023,45(2):88-96.
[22] 郭艳春,姚嘉瑜,张镕斌,等.中国黄麻炭疽病病原菌的分离鉴定及系统发育分析[J].作物学报,2022,48(3):770-777.
[23] 李晶,仇芳,张超,等.海南白沙和儋州油梨叶部及果实炭疽病菌的鉴定[J].果树学报,2023,40(7):1443-1454.
[24] 贡长怡,刘姣姣,邓强,等.茶树炭疽病病原菌鉴定及其致病性分析[J].园艺学报,2022,49(5):1092-1101.
[25] 石妞妞,阮宏椿,揭宇琳,等.福建省大豆炭疽病病原菌的分离与鉴定[J].植物保护学报,2022,49(2):539-546.
[26] 廖旺姣,韦维,邹东霞,等.广西香花油茶炭疽病病原菌鉴定及生物学特性[J].西南农业学报,2022,35(6):1340-1347.
[27] BEDLAN G.Erstnachweis von Colletotrichum dematium an Armoracia rusticana in Österreich[J].Journal für kulturpflanzen,2012,64(9):354-356.
[28] LEE D M, HASSAN O, KIM C H, et al.First report of anthracnose of peach (Prunus persica) caused by Colletotrichum fructicolain Korea[J]. Plant disease,2020,104(5):1556.
[29] 刘佳蓉,李楠,甘韬玉,等.百合炭疽病病原菌Colletotrichum spaethianum生物学特性及室内药剂筛选[J].中国蔬菜,2021(11):75-80.
[30] 王迪,王诗然,杨明佳,等.吉林省人参黑斑病菌对常用药剂的抗药性监测及交互抗药性测定[J].农药,2018,57(8):603-605.
[31] 潘俊,张焱珍,李晚谊,等.红葱抑制植物病原菌活性成分研究[J].西南农业学报,2011,24(6):2246-2248.
[32] 杨春霞,哈佳欣,徐克,等.胶孢炭疽菌生物防治研究进展[J].北方园艺,2023(5):132-139.
[33] 杨佩文,李艳琼,尚慧,等.放线菌发酵提取物对百合炭疽病的抗菌活性筛选[J].农药,2006,45(8):571-573.