Response characteristics of five enzymatic markers in earthworm （Amynthas heterochaetus） under Cd stress

doi:10.14088/j.cnki.issn0439-8114.2019.12.014

Abstract

Abstract: Amynthas heterochaetus, a widely distributed soil species in northern China, was analyzed under heavy metal cadmium （Cd） stress at a semi-lethal dose （LC₅₀）. The activities of Amynthas heterochaetus and its five immune-related enzyme markers under Cd stress were analyzed and compared with Eisenia fetida, a standard earthworm species recommended by OECD. The results showed that LC₅₀ of Amynthas heterochaetus（respectively 11.23 and 5.17 μg/cm²） of Cd at 24 and 48 h was lower than Eisenia fetida（respectively 21.99 and 16.95 μg/cm²）. Under the low concentration of Cd stress, the activities of SOD, GSH-PX, CAT, AchE and T-AOC were significantly shifted, both in Amynthas heterochaetus and Eisenia fetida. Compared to Eisenia fetida, the response of enzymatic biomarkers in Amynthas heterochaetus were quicker （3 days） and lasted longer （28 days）.

Key words: environmental benchmark, earthworm, Amynthas heterochaetus, Cd, enzyme markers

CLC Number:

X174

WU Jing-hui, LI Yong, WEI Yuan, YANG Xiao-jin, ZHANG Yi, HU Qing. Response characteristics of five enzymatic markers in earthworm （Amynthas heterochaetus） under Cd stress[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 58-63.

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