Abstract: The full-length porcine CD150 gene sequence was cloned and connected to the eukaryotic expression vector pEGFP-N1 to obtain the recombinant vector pEGFP-N1-CD150. The expression of pEGFP-N1-CD150 was confirmed by sequencing, Western blot and transient transfection. The results showed that the sequence comparison rate of pEGFP-N1-CD150 vector and CD150 gene sequence was 100%; when the density of Marc-145 cells was 70%~80%, the transfection peak was 24 h, and the green fluorescence was the strongest; the protein with molecular weight of about 80 kDa was identified by Western blot, which was consistent with the expected size, indicating that CD150 protein was successfully expressed.

Key words: porcine, CD150, eukaryotic expression