Screening PbTMT4 interaction factor by yeast two-hybrid system in pear

doi:10.14088/j.cnki.issn0439-8114.2019.24.058

Abstract

Abstract: To screen for the interaction factor of PbTMT4, a pear sugar transport related gene, based on the established cDNA library of Dangshan Suli pear fruit development period, using Matchmaker^TM Gold yeast two-hybrid syste m, the constructed recombinant plasmid pDHB1-PbTMT4 was used as a bait plasmid to transform yeast strain NMY51 by PEG/LiAc method. The phenotypic screening method was used to detect the self-activation and toxic effects of PbTMT4. The PbTMT4 gene interaction factor was screened using an amino acid-deficient medium. The results showed that the bait protein PbTMT4 was non-toxic and self-activating in the yeast two-hybrid system. Through yeast two-hybrid screening and sequencing and sequence alignment analysis, a total of 13 host proteins interacting with PbTMT4 protein were obtained.

Key words: PbTMT4, yeast two-hybrid system, cDNA library, interaction factor

CLC Number:

S661.2

CHENG Yin-sheng, YANG Li, CHEN Jian-qiu, ZHANG Shao-ling, WU Tao, ZHANG Hu-ping. Screening PbTMT4 interaction factor by yeast two-hybrid system in pear[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(24): 240-245.

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