蛋白质互作技术研究进展

doi:10.14088/j.cnki.issn0439-8114.2019.12.001

摘要/Abstract

摘要： 蛋白质作为细胞活性及功能的执行者,以复杂有序的动态互作协调着细胞的增殖与分化、衰老与死亡以及环境应答等各种重要生理过程。重点介绍了酵母双杂交系统、双分子荧光互补、噬菌体展示技术、荧光共振能量转移技术、谷胱甘肽巯基转移酶融合蛋白沉降技术、免疫共沉淀技术等蛋白质互作研究技术的原理及在生物学中的应用,以及生物信息学在蛋白质互作研究中的应用,并对目前及未来蛋白质互作技术的发展方向进行了探讨。

关键词: 蛋白质, 生物信息, 蛋白质互作

Abstract: Proteins,the true executer of cell activities and functions, play important roles in the proliferation, differentiation, aging and death, and environmental response via complex and highly ordered dynamic interaction. The basic principles and applications of several related research techniques in protein-protein interactions, including yeast two-hybrid system, bimolecular fluorescence complementation assay, phage display technology, fluoresence energy transfer, GST Pull-down, co-immunoprecipitation, and bioinformatics in protein interaction research applications, and their development prospects were also prospected.

Key words: protein, bioinformatics, protein interaction

中图分类号:

卢艳艳, 王超, 侍福梅. 蛋白质互作技术研究进展[J]. 湖北农业科学, 2019, 58(12): 5-10.

LU Yan-yan, WANG Chao, SHI Fu-mei. Research advances on protein interaction technology[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 5-10.

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