猪SPATA6基因的多态性与精液品质性状的关联分析

doi:10.14088/j.cnki.issn0439-8114.2019.24.039

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (24): 163-166.doi: 10.14088/j.cnki.issn0439-8114.2019.24.039

猪SPATA6基因的多态性与精液品质性状的关联分析

周佳伟, 吴俊静, 乔木, 刘贵生, 彭先文, 梅书棋

湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉 430064

收稿日期:2019-09-25 出版日期:2019-12-25 发布日期:2019-12-25
通讯作者: 梅书棋（1971-）,男,研究员,主要从事猪遗传育种研究,（电子信箱）msqlfe@163.com。
作者简介:周佳伟（1989-）,男,江西瑞昌人,助理研究员,博士,主要从事猪遗传育种研究,（电话）18674037082（电子信箱）422374839@qq.com
基金资助:
湖北省农业科技创新中心创新团队项目（2019-620-000-001-18）; 国家生猪产业技术体系项目（CARS-35）; 湖北省农业科技创新行动项目（2018skjcx05）; 湖北省自然科学基金创新群体项目（2018CFA014）; 湖北省技术创新专项重大项目（2019ABA084）; 湖北省农业科学院青年科学基金项目（2018NKYJJ09）

Association analysis of SPATA6 gene polymorphism and semenquality in porcine

ZHOU Jia-wei, WU Jun-jing, QIAO Mu, LIU Gui-sheng, PENG Xian-wen, MEI Shu-qi

Hubei Key Laboratory of Animal Embryo and Molecular Breeding/Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China

Received:2019-09-25 Online:2019-12-25 Published:2019-12-25

摘要/Abstract

摘要： 公猪精液品质直接影响到猪场经济效益,通过转录组测序发现SPATA6基因在猪性成熟前后的睾丸组织中差异表达,并发现其内含子区域内存在多个SNP位点。通过SNaPshot的方法分别对杜洛克猪和大白猪中SPATA6基因的rs331255092和rs341061477位点进行分型,并与精液品质性状进行关联分析。结果表明,在杜洛克猪和大白猪中rs331255092和rs341061477位点均与精子畸形率显著相关,此外,在大白猪中rs331255092和rs341061477位点也与精液量显著相关。猪SPATA6基因的rs331255092和rs341061477位点对猪的精液品质存在潜在调控作用。

关键词: 猪, SPATA6基因, SNP, 精子畸形率, 精液量

Abstract: The quality of boar semen directly affects the economic benefits of pig farms. Transcriptome sequencing showed that SPATA6 gene was differentially expressed in testicular tissues of porcine sexually mature and immature, and multiple SNP sites were found in its intron region. In this study, rs331255092 and rs341061477 loci of SPATA6 gene were identified by SNaPshot method in Duroc pig and Large White pig. The results showed that rs331255092 and rs341061477 were significantly correlated with sperm abnormality rate in Duroc pig and Large White pig. In addition, rs331255092 and rs341061477 were also significantly correlated with semen volume in Large White pig. In summary, rs331255092 and rs341061477 have potential regulatory effects on semen quality in pigs.

Key words: pig, SPATA6 gene, SNP, sperm abnormality rate, semen volume

中图分类号:

S828

周佳伟, 吴俊静, 乔木, 刘贵生, 彭先文, 梅书棋. 猪SPATA6基因的多态性与精液品质性状的关联分析[J]. 湖北农业科学, 2019, 58(24): 163-166.

ZHOU Jia-wei, WU Jun-jing, QIAO Mu, LIU Gui-sheng, PENG Xian-wen, MEI Shu-qi. Association analysis of SPATA6 gene polymorphism and semenquality in porcine[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(24): 163-166.

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猪SPATA6基因的多态性与精液品质性状的关联分析

Association analysis of SPATA6 gene polymorphism and semenquality in porcine

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