剩余污泥碱性发酵产蛋白酶菌株的筛选与鉴定

doi:10.14088/j.cnki.issn0439-8114.2020.06.029

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (6): 143-147.doi: 10.14088/j.cnki.issn0439-8114.2020.06.029

剩余污泥碱性发酵产蛋白酶菌株的筛选与鉴定

接伟光^{1, 2}, 姚延轩², 彭永臻^{1, 3}, 胡崴²

1.哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨 150090;
2.黑龙江东方学院食品与环境工程学部,哈尔滨 150066;
3.北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京 100124

收稿日期:2019-06-19 出版日期:2020-03-25 发布日期:2020-06-13
通讯作者: 接伟光（1981-）,男,黑龙江哈尔滨人,副教授,博士,主要从事污泥碱性厌氧发酵研究,（电话）13104519855（电子信箱）jieweiguang2007@126.com;通信作者,彭永臻（1949-）,男,教授,博士生导师,（电话）18245135098（电子信箱）pyz@bjut.edu.cu。
作者简介:接伟光（1981-）,男,黑龙江哈尔滨人,副教授,博士,主要从事污泥碱性厌氧发酵研究,（电话）13104519855（电子信箱）jieweiguang2007@126.com;
基金资助:
国家技术研究发展计划项目（2012AA063406; 2011AA060903-02）

Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge

JIE Wei-guang^{1, 2}, YAO Yan-xuan², PENG Yong-zhen^{1, 3}, HU Wei²

1.National Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China;
2.Department of Food and Environment Engineering,East University of Heilongjiang,Harbin 150066,China;
3.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China

Received:2019-06-19 Online:2020-03-25 Published:2020-06-13

摘要/Abstract

摘要： 为了能够缩短剩余污泥碱性发酵周期,从碱性发酵剩余污泥中筛选产蛋白酶活力较高的耐碱细菌,利用形态学、生理生化、分子生物学特征对筛选到的菌株进行鉴定。结果表明,从碱性厌氧发酵剩余污泥中最终筛选获得2株产蛋白酶活力较高的耐碱细菌HIT-01菌株和HIT-02菌株,经鉴定分别为枯草芽孢杆菌（Bacillus subtilis）和芽孢杆菌属细菌（Bacillus sp.）。为进一步利用筛选获得的优势菌株构建生物菌剂、从而快速启动污泥碱性发酵开拓了新思路。

关键词: 剩余污泥, 产蛋白酶菌株, 筛选, 鉴定

Abstract: In order to shorten the alkaline fermentation period of excess sludge, alkaline-tolerant strains with high protease-producing activity were isolated from alkaline fermentation of excess sludge and identified by morphological. physiological, biochemical, and molecular characteristic analysis. The results showed that the two screened alkali-tolerant bacterial strains （HIT-01 and HIT-02 strains） which of high protease-producing activity were belongs to Bacillus subtilis and Bacillus sp. This study opens a new idea for further utilizing the screened dominant strains to construct microbial agents and quickly activate alkaline fermentation of sludge.

Key words: excess sludge, protease-producing bacteria, screening, identification

中图分类号:

中图法分类号：X703 文献标识码：A

接伟光, 姚延轩, 彭永臻, 胡崴. 剩余污泥碱性发酵产蛋白酶菌株的筛选与鉴定[J]. 湖北农业科学, 2020, 59(6): 143-147.

JIE Wei-guang, YAO Yan-xuan, PENG Yong-zhen, HU Wei. Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(6): 143-147.

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剩余污泥碱性发酵产蛋白酶菌株的筛选与鉴定

Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge

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