湖北农业科学 ›› 2020, Vol. 59 ›› Issue (8): 138-140.doi: 10.14088/j.cnki.issn0439-8114.2020.08.031

• 检测分析 • 上一篇    下一篇

芒果果肉中总黄酮及芒果苷含量的测定

刘常凯, 杨黎, 何林飞   

  1. 广西-东盟食品药品安全检验检测中心,南宁 530021
  • 收稿日期:2019-07-15 出版日期:2020-04-25 发布日期:2020-07-03
  • 通讯作者: 何林飞,主管药师,主要从事食品药品质量与安全研究工作,(电子信箱)2960215496@qq.com。
  • 作者简介:刘常凯(1986-),女,广西博白人,主管药师,主要从事食品药品质量与安全研究工作,(电话)15296388369(电子信箱)373719196@qq.com。

Determination of total flavonoids and mangiferin in mango flesh

LIU Chang-kai, YANG Li, HE Lin-fei   

  1. Guangxi-Asean Food and Drug Safety Inspection and Testing Center,Nanning 530021,China
  • Received:2019-07-15 Online:2020-04-25 Published:2020-07-03

摘要: 建立芒果(Mangifera indica L.)果肉中总黄酮及芒果苷含量的测定方法,采用紫外-分光光度计法测定总黄酮;采用高效液相色谱法测定芒果苷。结果表明,总黄酮在17~520 μg/mL浓度范围内呈现良好线性关系(R2=0.999 9),平均回收率为99.03%,RSD为1.42%(n=6);芒果苷在4.0~80.0 μg/mL浓度范围内呈现良好线性关系(R=0.999 9),平均回收率为101.59%,RSD为1.87%(n=6)。该方法简便、准确度高、重复性良好,可用于芒果果肉中总黄酮及芒果苷的测定。

关键词: 总黄酮, 芒果苷, 紫外-分光光度计, 液相色谱法

Abstract: A method for the determination of total flavonoids and mangiferin in mango flesh was established. The total flavonoids in mango flesh were determined by uv-spectrophotometer. Mangiferin was determined by high performance liquid chromatography. The results showed that the total flavonoids had a good linear relationship in the concentration range of 17~520 μg/mL (r=0.999 9), the average recovery was 99.03%, and the RSD was 1.42% (n=6). Mangiferin showed a good linear relationship in the concentration range of 4.0~80 μg/mL (r=0.999 9), with an average recovery of 101.59% and RSD of 1.87% (n=6). The method developed in this paper was simple, accurate and reproducible. It can be used for the determination of total flavonoids and mangiferin in mango flesh.

Key words: total flavonoids, mangiferin, uv-spectrophotometer, liquid chromatography

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