臭椿canthin-6-one对禾谷镰刀菌的抑菌作用

doi:10.14088/j.cnki.issn0439-8114.2024.09.015

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (9): 84-88.doi: 10.14088/j.cnki.issn0439-8114.2024.09.015

臭椿canthin-6-one对禾谷镰刀菌的抑菌作用

赵美荣, 李永春, 李春颖

赤峰学院化学与生命科学学院,内蒙古赤峰 024000

收稿日期:2023-04-20 出版日期:2024-09-25 发布日期:2024-09-30
作者简介:赵美荣（1981-）,女,山东嘉祥人,副教授,博士,主要从事植物次生代谢物质开发利用研究,（电话）15047639680（电子信箱）738532543@qq.com。
基金资助:
内蒙古自治区高等学校科学研究项目（NJZY21137; NJZZ22150）; 赤峰学院一流学科建设项目（CFXYYLXKB202102）; 蒙东地区生物资源开发利用创新团队项目（FXYKYCXTD202008）

Antifungal effect of canthin-6-one from Ailanthus altissima on Fusarium graminearum

ZHAO Mei-rong, LI Yong-chun, LI Chun-ying

College of Chemistry and Life Science, Chifeng University, Chifeng 024000, Inner Mongolia, China

Received:2023-04-20 Published:2024-09-25 Online:2024-09-30

摘要/Abstract

摘要： 为进一步研究臭椿（Ailanthus altissima）canthin-6-one生物碱对禾谷镰刀菌（Fusarium graminearum）的抑菌效果及其作用机制,采用微量稀释法、菌丝生长抑制法测定其抑菌活性,并通过测定胞外相对电导率、内容物释放量、丙二醛含量、过氧化氢含量以及保护酶活性,从细胞膜角度分析其抑菌作用机制。结果显示,canthin-6-one对禾谷镰刀菌具有显著的抑菌作用,其最小抑菌浓度（MIC）为8.0 μg/mL,半数有效浓度（EC₅₀）为1.85 μg/mL。处理后的菌体胞外相对电导率和内容物释放量显著增加,同时菌体丙二醛和过氧化氢含量亦有显著升高。这表明canthin-6-one引起了禾谷镰刀菌细胞膜氧化损伤,导致膜完整性丧失及透性增强,从而抑制菌丝生长。

关键词: 臭椿（Ailanthus altissima）, 禾谷镰刀菌（Fusarium graminearum）, 生物碱, 抑菌作用, 细胞膜

Abstract: To further study the fungicidal effect and mechanism of canthin-6-one alkaloid from Ailanthus altissima on Fusarium graminearum, the inhibitory activity was determined by microdilution and mycelial growth inhibition methods. The antifungal mechanism of canthin-6-one was analyzed from the point of view of cell membrane by detecting the extracellular relative conductivity, content release, malondialdehyde content, hydrogen peroxide content and protective enzyme activity. The results showed that canthin-6-one had a significant inhibitory effect on Fusarium graminearum, with a minimum inhibitory concentration of 8.0 μg/mL and a half-effective concentration of 1.85 μg/mL. The extracellular relative conductivity and the content release amount of the treated mycelia were significantly increased, while malondialdehyde and hydrogen peroxide contents were also significantly elevated. It was indicated that canthin-6-one induced oxidative damage to the cell membrane of Fusarium graminearum, resulting in the loss of membrane integrity and increased permeability, thus inhibiting mycelial growth.

Key words: Ailanthus altissima, Fusarium graminearum, alkaloid, antifungal effect, cell membrane

中图分类号:

S482.2

赵美荣, 李永春, 李春颖. 臭椿canthin-6-one对禾谷镰刀菌的抑菌作用[J]. 湖北农业科学, 2024, 63(9): 84-88.

ZHAO Mei-rong, LI Yong-chun, LI Chun-ying. Antifungal effect of canthin-6-one from Ailanthus altissima on Fusarium graminearum[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(9): 84-88.

参考文献

[1] JIMENEZ-QUIROS C,OKECHUKWU E C,HONG Y G,et al.Comparison of antifungal activity of Bacillus strains against Fusarium graminearum in vitro and in planta[J].Plants,2022,11(15):1999.
[2] ZHANG W J,GAO F K,CHENG C H,et al.Evaluation of sulfonated oxidized chitosan antifungal activity against Fusarium graminearum[J].Journal of biomaterials science, polymer edition,2022,33(12):1495-1510.
[3] NGUVO K J,GAO X Q.Weapons hidden underneath: Bio-control agents and their potentials to activate plant induced systemic resistance in controlling crop Fusarium diseases[J].Journal of plant diseases and protection,2019,126(3):177-190.
[4] 潘漫,盛园园,程傲星,等.8种中药提取物对植物病原真菌的抑制活性[J].资源开发与市场, 2011,27(5):394-396.
[5] 周江鸿,夏菲,车少臣.16种园林植物粗提物对黄栌枯萎病菌的抑制作用[J].园林科技,2015(4):30-34.
[6] 胡苗芬,宋新波,张丽娟.臭椿吲哚生物碱成分及其生物活性研究进展[J].药物评价研究,2012, 35(6):469-472.
[7] 邵青,权伟,高凯,等.臭椿生物碱canthin-6-one对小鼠镇静催眠作用的研究[J].现代生物医学进展,2020,20(1):46-49,67.
[8] THOUVENEL C,GANTIER J C,DURET P,et al.Antifungal compounds from Zanthoxylum chiloperone var. angustifolium[J].Phytotherapy research,2003,17(6):678-680.
[9] GAZONI V F,BALOGUN S O,ARUNACHALAM K,et al.Assessment of toxicity and differential antimicrobial activity of methanol extract of rhizome of Simaba ferruginea A. St.-Hil. and its isolate canthin-6-one[J].Journal of ethnopharmacology,2018,223(15):122-134.
[10] LI Y C,ZHAO M R,ZHANG Z.Quantitative proteomics reveals the antifungal effect of canthin-6-one isolated from Ailanthus altissima against Fusarium oxysporum f. sp. cucumerinum in vitro[J]. PLoS one,2021,16(4):e0250712.
[11] 李永春,赵美荣,张智.臭椿生物碱对5种植物病原真菌的抑制作用[J].福建农业学报,2021, 36(1):53-58.
[12] 毕亚琪,王禹贺,罗泗川,等. MB和CMC液体培养基对禾谷镰孢产孢水平的影响[J].植物保护, 2020,46(6):155-158.
[13] LIU X M,OUYANG C B,WANG Q X,et al.Evaluation of antibacterial and antifungal properties of 9-oxo-10,11-dehydroageraphorone extracted from Eupatorium adenophorum[J].Journal of plant diseases and protection,2016,123:93-99.
[14] 李永春. 臭椿canthin-6-one的提取分离及对尖孢镰刀菌抑菌机制研究[D].哈尔滨:东北林业大学,2021.
[15] ZHOU H E,TAO N G,JIA L.Antifungal activity of citral, octanal and α-terpineol against Geotrichum citri-aurantii[J].Food control,2014,37:277-283.
[16] BALLESTER A R,LAFUENTE M T,GONZÁLEZ-CANDELAS L. Spatial study of antioxidant enzymes, peroxidase and phenylalanine ammonia-lyase in the citrus fruit-Penicillium digitatum interaction[J].Postharvest biology & technology,2006,39(2):115-124.
[17] CHOWHAN N,SINGH H P,BATISH D R,et al.β-Pinene inhibited germination and early growth involves membrane peroxidation[J].Protoplasma,2013,250(3):691-700.
[18] 区敏仪,李海涛,杨蓉娅.真菌抗氧化酶的研究进展[J].国际皮肤性病学杂志,2017,43(1):50-53.
[19] 刘晓环,张朝晖.抗真菌药物作用靶点机理及新药研发进展[J].药物分析杂志,2015,35(2):193-202.
[20] LUO N X,JIN L,YANG C Q,et al.Antifungal activity and potential mechanism of magnoflorine against Trichophyton rubrum[J].The journal of antibiotics,2020,74(3):206-214.
[21] ZHAO Z M,SHANG X F,LAWOE R K,et al.Anti-phytopathogenic activity and the possible mechanisms of action of isoquinoline alkaloid sanguinarine[J].Pesticide biochemistry and physiology,2019,159:51-58.
[22] MARTINEZ-FINLEY E J,GAVIN C E,ASCHNER M,et al. Manganese neurotoxicity and the role of reactive oxygen species[J].Free radical biology and medicine,2013,62:65-75.
[23] 魏立强,谢慧琴,杨德松,等.旱芹粗提物对棉花枯萎病菌丙二醛含量、电导率及保护酶活性的影响[J].植物保护,2012,38(3):28-31.
[24] 王欣,沈亚伦,李云鹏,等.川芎萃取物对蓝莓叶斑病菌的抑制作用[J].中国植保导刊,2022, 42(6):5-9.

臭椿canthin-6-one对禾谷镰刀菌的抑菌作用

Antifungal effect of canthin-6-one from Ailanthus altissima on Fusarium graminearum

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