乙草胺和丁草胺对斑马鱼前列腺素代谢产物的影响

doi:10.14088/j.cnki.issn0439-8114.2025.06.017

摘要/Abstract

摘要： 采用100 μg/L乙草胺(AC)和丁草胺(BC),对成年斑马鱼(Danio rerio)雌鱼、雄鱼进行96 h染毒处理,运用代谢组学技术研究乙草胺和丁草胺对斑马鱼前列腺素(PGs)代谢产物的影响。结果表明,建立超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法,在6个处理[AC处理的雌鱼(ACF)、雄鱼(ACM),BC处理的雌鱼(BCF)、雄鱼(BCM),对照雌鱼(CKF)和雄鱼(CKM)]的24个样品中共检测出1 683种代谢物。对总代谢产物的火山图分析发现,除BCM与CKM处理外,其余处理显著上调的代谢物数量明显多于显著下调的代谢物数量。前列腺素代谢产物(PGs)分析表明,在ACF处理下,16个PGs代谢物显著上调,1个iPG显著下调;ACM处理下,9个PGs代谢产物显著上调。BCF处理染毒后,18个PGs代谢物显著上调;BCM处理下,2个PGs代谢产物显著上调。

关键词: 斑马鱼(Danio rerio), 前列腺素, 代谢产物, 乙草胺, 丁草胺

Abstract: Adult female and male zebrafish(Danio rerio) were exposed to 100 μg/L of acetochlor(AC) and butachlor (BC) for 96 h, and the effects of acetochlor or butachlor on the prostaglandin (PGs) metabolites of zebrafish were investigated using metabolomics techniques. The results showed that an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection method was established, and a total of 1 683 metabolites were detected from the 24 samples of the six treatments[AC treated female(ACF) and male fish(ACM),BC treated female（BCF）and male fish(BCM),control female(CKF) and male fish(CKM)]. Volcano plot analysis of all metabolites revealed that except for BCM and CKM treatments,the number of significantly up-regulated metabolites was notably higher than that of down-regulated ones. Prostaglandin metabolite (PGs) analysis showed that under ACF treatment, 16 PGs metabolites were significantly up-regulated, and iPGs were significantly down-regulated; under ACM treatment, 9 PGs metabolites were significantly up-regulated. After BCF treatment, 18 PGs metabolites were significantly up-regulated; after BCM treatment, 2 PGs metabolites were significantly up-regulated.

Key words: zebrafish(Danio rerio), prostaglandins, metabolites, acetochlor, butachlor

中图分类号:

周新宗, 李少斌, 田晓丽, 常菊花. 乙草胺和丁草胺对斑马鱼前列腺素代谢产物的影响[J]. 湖北农业科学, 2025, 64(6): 103-109.

ZHOU Xin-zong, LI Shao-bin, TIAN Xiao-li, CHANG Ju-hua. Effects of acetochlor and butachlor on prostaglandin metabolites in zebrafish[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 103-109.

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