肉桂和丁香中α-葡萄糖苷酶抑制剂的筛选

doi:10.14088/j.cnki.issn0439-8114.2025.03.017

摘要/Abstract

摘要： 为探究药食同源植物肉桂和丁香中具有α-葡萄糖苷酶抑制作用的潜在活性物质,采用体外酶活性模型实验测定肉桂和丁香甲醇粗提物对α-葡萄糖苷酶的抑制作用,进一步采用生物活性色谱和超高效液相色谱-串联四级杆飞行时间质谱(UPLC-QTOF-MS/MS)分析其中的活性物质。结果表明,肉桂和丁香甲醇粗提物具有较强的α-葡萄糖苷酶抑制作用,IC₅₀分别为(50.0±0.08) μg/mL和(2.19±0.17) μg/mL,抑制作用明显高于阳性对照阿卡波糖[IC₅₀=(264.37±2.73) μg/mL]。生物活性色谱分析表明多酚类物质为主要活性成分。UPLC-QTOF-MS/MS分析揭示丁香中的黄酮类物质、肉桂中的肉桂酸和2-甲氧基肉桂醛可能为潜在的活性成分。肉桂和丁香在预防和治疗糖尿病的功能性利用方面具有一定潜力。

关键词: 药食同源植物, α-葡萄糖苷酶, 生物活性色谱, 超高效液相色谱-串联四级杆飞行时间质谱(UPLC-QTOF-MS/MS)

Abstract: The purpose of this study was to explore the potential constituents responsible for α-glucosidase inhibitory activity of medicine food homologous herbs Eugenia caryophyllata and Cinnamomum cassia. The α-glucosidase inhibitory effect of crude extracts from Eugenia caryophyllata and Cinnamomum cassia were preliminary determined by in vitro enzyme activity experiment. The corresponding bioactive constituents were determined by the combined use of high-resolution inhibition profiling and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). The results showed that, crude methanol extracts of Eugenia caryophyllata and Cinnamomum cassia displayed considerable inhibition towards α-glucosidase with IC₅₀values of (50.0±0.08) μg/mL and (2.19±0.17) μg/mL, respectively, more active than the positive control acarbose [IC₅₀=(264.37±2.73) μg/mL]. The high-resolution inhibition profiles pointed out that polyphenols were correlated with α-glucosidase inhibitory activity. In addition, flavonoids from Eugenia caryophyllata, cinnamic acid and 2′-methoxy cinnamaldehyde from Cinnamomum cassia were considered as the possible inhibitors by the careful analysis of UPLC-QTOF-MS/MS data. The results supported further research with the aim of developing Eugenia caryophyllata and Cinnamomum cassia into functional food for prevention and management of T2D and T2D-related complications.

Key words: medicine food homologous herbs, α-glucosidase, high-resolution inhibition profiling, ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)

中图分类号:

R284.1

徐聪. 肉桂和丁香中α-葡萄糖苷酶抑制剂的筛选[J]. 湖北农业科学, 2025, 64(3): 107-111.

XU Cong. Screening of α-glucosidase inhibitors in Eugenia caryophyllata and Cinnamomum cassia[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(3): 107-111.

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