青蒿组织培养一步成苗技术体系的建立与优化

doi:10.14088/j.cnki.issn0439-8114.2026.06.035

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (6): 227-233.doi: 10.14088/j.cnki.issn0439-8114.2026.06.035

青蒿组织培养一步成苗技术体系的建立与优化

杨胜妃^1a,1b, 张梅², 彭壹^1a,1b, 罗惠玲^1a,1b, 彭学东², 蔡诗云³, 丁远杰^1b,1c

1.吉首大学,a.旅游与城乡规划学院; b.林产化工工程湖南省重点实验室; c.杜仲综合利用技术国家地方联合工程实验室,湖南张家界 427000;
2.湖南斯依康生物科技有限公司,湖南永州 425000;
3.澧县农业农村局,湖南常德 415000

收稿日期:2026-01-19 出版日期:2026-06-25 发布日期:2026-06-26
通讯作者: 丁远杰（1977-）,男,湖南常德人,讲师,博士,主要从事药用植物资源开发利用与栽培研究,（电子信箱）dingyuanjie@jsu.edu.cn。
作者简介:杨胜妃（2002-）,女,湖南衡阳人,在读硕士研究生,研究方向为植物组织培养与栽培,（电子信箱）2864237642@qq.com。
基金资助:
国家自然科学基金项目（31760084）; 湖南省科技特派员服务乡村振兴项目（2024RC8254）; 湖南省中医药科研重点项目（A2023045）; 吉首大学2019年引进人员科研资助经费项目（1119051）

Establishment and optimization of a one-step plantlet regeneration system for tissue culture of Artemisia annua L.

YANG Sheng-fei^1a,1b, ZHANG Mei², PENG Yi^1a,1b, LUO Hui-ling^1a,1b, PENG Xue-dong², CAI Shi-yun³, DING Yuan-jie^1b,1c

1a. School of Tourism and Urban-Rural Planning; 1b. Key Laboratory of Hunan Forest and Chemical Industry Engineering; 1c. National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, Jishou University, Zhangjiajie 427000, Hunan, China;
2. Hunan Siyikang Biotechnology Co., Ltd., Yongzhou 425000, Hunan, China;
3. Lixian Agriculture and Rural Bureau, Changde 415000, Hunan, China

Received:2026-01-19 Published:2026-06-25 Online:2026-06-26

摘要/Abstract

摘要： 为简化青蒿（Artemisia annua L.）组织培养种苗生产流程、缩短培育周期,建立青蒿一步成苗技术体系。以青蒿带腋芽茎段为试验材料,采用2因素多水平完全随机设计,优化丛生芽诱导、增殖与生根阶段的植物生长调节剂浓度组合,并探究炼苗时间对幼苗移栽成活率及长势的影响。结果表明,以青蒿带芽嫩茎为外植体,经流水冲洗30 min后,采用0.1%氯化汞溶液灭菌处理12 min时,青蒿外植体成活率最高（86.67%）,褐化率维持在较低水平。以MS + 4 mg/L 6-BA为诱导培养基可直接诱导丛生芽形成,丛生芽诱导率达75.56%,芽点形成时间缩短至9.67 d,能有效促进青蒿一步成苗。以MS + 3 mg/L 6-BA为增殖培养基进行继代培养,增殖系数达7.37,且明显延缓玻璃化现象发生。试管苗的最适生根培养基为MS + 1 mg/L NAA,生根率达97.78%,平均生根数14.67条/株。组培苗移栽炼苗的最适方案为闭瓶炼苗2 d后,再经盆栽覆膜炼苗7 d,移栽成活率达96.59%。该青蒿一步成苗技术体系操作流程简单、繁殖效率高,有助于实现青蒿种苗的规模化生产。

关键词: 青蒿（Artemisia annua L.）, 组织培养, 一步成苗, 技术体系, 生根培养, 炼苗移栽

Abstract: To simplify the seedling production process of tissue culture of Artemisia annua L. and shorten the cultivation period, a one-step plantlet regeneration system for Artemisia annua L. was established. Using stem segments with axillary buds of Artemisia annua L. as experimental materials, a two-factor multilevel completely randomized design was adopted to optimize the concentration combinations of plant growth regulators at the stages of multiple shoot induction, proliferation, and rooting, and the effects of acclimatization duration on the survival rate and growth vigor of transplanted plantlets were investigated. The results showed that when tender stems with buds of Artemisia annua L. were used as explants, rinsed with running water for 30 min, and then sterilized with 0.1% mercuric chloride solution for 12 min, the survival rate of explants reached the highest level (86.67%), and the browning rate was maintained at a relatively low level. Using MS + 4 mg/L 6-BA as the induction medium, multiple shoot formation was directly induced, with a multiple shoot induction rate of 75.56% and the time for bud formation shortened to 9.67 d, which effectively promoted the one-step plantlet regeneration of Artemisia annua L. Using MS + 3 mg/L 6-BA as the proliferation medium for subculture, the proliferation coefficient reached 7.37, and the occurrence of vitrification was significantly delayed. The optimal rooting medium for test-tube plantlets was MS + 1 mg/L NAA, with a rooting rate of 97.78% and an average root number of 14.67 per plant. The optimal acclimatization protocol for transplanting tissue-cultured plantlets involved 2 days of closed-bottle acclimatization followed by 7 days of potted acclimatization with plastic film mulching, achieving a transplanting survival rate of 96.59%. This one-step plantlet regeneration system for Artemisia annua L. was characterized by a simple operational process and high propagation efficiency, which facilitated the large-scale production of Artemisia annua L. plantlets.

Key words: Artemisia annua L., tissue culture, one-step plantlet regeneration, technical system, rooting culture, acclimatization and transplanting

中图分类号:

S567.21⁺9

杨胜妃, 张梅, 彭壹, 罗惠玲, 彭学东, 蔡诗云, 丁远杰. 青蒿组织培养一步成苗技术体系的建立与优化[J]. 湖北农业科学, 2026, 65(6): 227-233.

YANG Sheng-fei, ZHANG Mei, PENG Yi, LUO Hui-ling, PENG Xue-dong, CAI Shi-yun, DING Yuan-jie. Establishment and optimization of a one-step plantlet regeneration system for tissue culture of Artemisia annua L.[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(6): 227-233.

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青蒿组织培养一步成苗技术体系的建立与优化

Establishment and optimization of a one-step plantlet regeneration system for tissue culture of Artemisia annua L.

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