湖北农业科学 ›› 2022, Vol. 61 ›› Issue (15): 218-221.doi: 10.14088/j.cnki.issn0439-8114.2022.15.041

• 生物工程 • 上一篇    下一篇

欧月红宝石冰组织培养快繁技术研究

李芬, 刘木清, 祝剑峰   

  1. 湖北生态工程职业技术学院,武汉 430200
  • 收稿日期:2022-03-25 出版日期:2022-08-10 发布日期:2022-08-25
  • 通讯作者: 祝剑峰(1981-),男,副教授,硕士,主要从事植物生物技术研究,(电话)15671669362(电子信箱)27352822@qq.com。
  • 作者简介:李 芬(1984-),女,湖北随州人,讲师,硕士,主要从事园林、花艺研究,(电话)13296508555(电子信箱)375450868@qq.com

Research on tissue culture and rapid propagation technology of European rose ruby ice

LI Fen, LIU Mu-qing, ZHU Jian-feng   

  1. Hubei Ecology Vocational College,Wuhan 430200,China
  • Received:2022-03-25 Online:2022-08-10 Published:2022-08-25

摘要: 以欧月(Rosa chinensis Jacq.)品种红宝石冰为试验材料,取其半木质化的中部茎段作为外植体,成功建立了组织培养技术体系。结果表明,外植体最佳的灭菌方式是采用0.1% HgCl2灭菌8 min;初代培养阶段最佳培养基配方为MS+0.2 mg/L NAA+2.0 mg/L 6-BA,继代增殖培养阶段最佳培养基配方为MS+0.2 mg/L NAA+2.5 mg/L 6-BA,生根培养阶段最佳培养基配方为1/2 MS+0.2 mg/L NAA;移栽的最佳基质为珍珠岩+草炭+蛭石,体积比为1∶1∶1。

关键词: 欧月(Rosa chinensis Jacq.), 组织培养, 快繁

Abstract: The semi-lignified middle stem segments of European rose (Rosa chinensis Jacq.)variety ruby ice were used as explants, and the tissue culture system was successfully established. The results showed that the best sterilization method for explants was 0.1% HgCl2 for 8 min; the optimal medium formula in the primary culture stage was MS+0.2 mg/L NAA+2.0 mg/L 6-BA, the optimal medium formula in the subculture proliferation stage was MS+0.2 mg/L NAA+2.5 mg/L 6-BA, and the optimal medium formula in the rooting culture stage was 1/2 MS+0.2 mg/L NAA. The best transplanting substrate was perlite+peat+vermiculite, and the volume ratio was 1∶1∶1.

Key words: European rose(Rosa chinensis Jacq.), tissue culture, rapid reproduction

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