湖北农业科学 ›› 2022, Vol. 61 ›› Issue (15): 214-217.doi: 10.14088/j.cnki.issn0439-8114.2022.15.040

• 生物工程 • 上一篇    下一篇

灰楸组织培养体系研究

杨玉珍1, 李晓哲2, 侯宏伟2   

  1. 1.南阳农业职业学院植物组织培养中心,河南 南阳 473000;
    2.中国科学院水生生物研究所,武汉 430072
  • 收稿日期:2021-10-09 出版日期:2022-08-10 发布日期:2022-08-25
  • 通讯作者: 侯宏伟(1968-),男,研究员,从事水生植物生理生态及应用研究,(电子信箱)houhw@ihb.ac.cn。
  • 作者简介:杨玉珍(1972-),女,河南南阳人,副教授,硕士,从事农林专业教学与植物组培研究,(电话)13721846378(电子信箱)yzyang128@126.com
  • 基金资助:
    河南省教育厅科研项目(2021SJGLX911); 南阳市科技攻关项目(KJGG138)

Study on tissue culture system of Catalpa fargesii Bureau

YANG Yu-zhen1, LI Xiao-zhe2, HOU Hong-wei2   

  1. 1. Center of Plant Tissue Culture, Nanyang Vocational College of Agriculture,Nanyang 473000, Henan,China;
    2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  • Received:2021-10-09 Online:2022-08-10 Published:2022-08-25

摘要: 楸树(Catalpa bungei C. A. Mey.)是中国特有的优良乡土树种和著名的园林观赏树种。以河南南阳乡土树种灰楸(Catalpa fargesii Bureau)优选株茎段为外植体,研究诱导丛生芽,壮苗生根。结果表明,丛生芽诱导增殖最佳生长调节剂配比为MS+1.0 mg/L 6-BA+0.5 mg/L GA3+0.5 mg/L IBA,壮苗培养的最佳生长调节剂配比为MS+0.6 mg/L 6-BA+1.0 mg/L IBA,幼苗生根以1/2 MS+0.5 mg/L IBA为最佳。

关键词: 灰楸(Catalpa fargesii Bureau), 组织培养, 快速繁殖

Abstract: Catalpa bungei C.A. Mey. is a unique native tree species and famous ornamental tree species in China. The optimal stem segments of native species of Catalpa fargesii Bureau in Nanyang, Henan were used as explants to study the induction of cluster buds and rooting of strong seedlings. The results showed that the optimal ratio of growth regulators for the induction and proliferation of clustered buds was MS+1.0 mg/L 6-BA+0.5 mg/L GA3+0.5 mg/L IBA. The optimal ratio of growth regulators for the cultivation of strong seedlings was MS+0.6 mg/L 6-BA+1.0 mg/L IBA. The optimal rooting rate of seedlings was 1/2 MS+0.5 mg/L IBA.

Key words: Catalpa fargesii Bureau, tissue culture, rapid propagation

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