Abstract: To develop raw materials for cultivation of Ganoderma atrum J. D.Zhao, L. W. Hsu et X. Q. Zhang, the grape（Vitis L.） sawdust and Pleurotus eryngii （DC.ex.Fr.） Quel. waste residue was used. The results showed that the grape sawdust is rich in cellulose and lignin; while P. eryngii waste residue contains high nitrogen free extract and crude protein; reasonable combination of P. eryngii waste residue and grape sawdust has a certain role in promoting the growth of G. atrum. On medium containing grape sawdust and cotton seed shell（60∶20 and 50∶30, W/W）,88 and 92 mm/d of mycelial growth rate, and 58.9% and 63.1% of biological conversion rate were achieved, respectively, significantly higher than the control. When 200 to 300 g/kg P. eryngii waste residue was added in medium containing 60 grape sawdust and 20 cottonseed shell, the mycelium growth rate could be 101 to 104 mm/d; time for mycelia to fulfill the bag could be shortened by 14 to 16 d; and biological conversion rate could reach to 79.6% to 82.3%.

Key words: Ganoderma atrum J. D.Zhao, L. W. Hsu et X. Q. Zhang, cultivation, grape（Vitis L.） sawdust, Pleurotus eryngii （DC.ex.Fr.） Quel. waste residue