Effects of pH and Salinity on Growth and Dissolution of Chlorella vlgaris

doi:10.14088/j.cnki.issn0439-8114.2018.11.020

Abstract

Abstract: Based on the use of Chlorella vlgaris in different environmental factors pH and salinity conditions,determination of dissolved oxygen and liquid algae growth, preliminary determination of Chlorella vlgaris has wide adaptability to salinity, growth in 0 to 45 in the salinity of sea water can, in which salinity 25 of the dissolved oxygen and the largest amount of growth to the largest data,the highest value of dissolved oxygen content and growth were 7.65 mg/L and 2.65×10⁷/mL, Chlorella vlgaris at pH 5.5~11.5 environment can survive at pH 9 dissolved oxygen and growth of most high the highest the data here, the highest value of dissolved oxygen content and growth were 8.04 mg/L and 2.5×10⁷/mL, the pH is greater than chlorella growth slowed significantly in 11, and even the phenomenon of death.

Key words: Chlorella vlgaris, pH, salinity, growth, dissolved oxygen

CLC Number:

Q948.8

ZHANG Qi, CAO Ying-kun, XING Ze-yu, LIANG Yan, SHEN Qing-zhou. Effects of pH and Salinity on Growth and Dissolution of Chlorella vlgaris[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(11): 83-86.

References

[1] 胡鸿钧,魏印心.中国淡水藻类系统、分类及生态[M].北京:科学出版社,2006.
[2] 华汝成. 单细胞藻类的培养与利用[M].北京:农业出版社,1986.
[3] 王逸云,王长海.无菌条件下的小球藻培养条件优化[J].烟台大学学报(自然科学与工程版),2006,19(2):125-129.
[4] 胡开辉,汪世华.小球藻的研究开发进展[J].武汉工业学院学报,2005,24(3):27-30.
[5] 李师翁,李虎乾.植物单细胞蛋白资源小球藻开发利用研究的现状[J].生物技术,1997,7(3):45-48.
[6] 陈颖,李文彬,孙勇如.小球藻生物技术研究应用现状及展望[J].生物工程进展,1998,18(6):12-16.
[7] 李志勇,郭祀远,李琳,等.微藻保健食品的开发与利用[J].食品研究与开发,1997,18(2):38-40.
[8] 欧阳峥嵘,温小斌,耿亚红.光照强度、温度、pH、盐度对小球藻(Chlorella)光合作用的影响[J].武汉植物学研究,2010,28(1): 49-55.
[9] 赵彦坤,张文胜,王幼宁,等.高pH对植物生长发育的影响及其分子生物学研究进展[J].中国生态农业学报,2008,16(3):783-787.
[10] BHATII S,HUERTAS I E,COLMAN B.Acquisition of inorganic carbon by the marine haptophyte Isochrysis galbana (Prymnesio-phyceae)[J].Journal of Phycology,2002,38(5): 914-921.
[11] WILLIAMS T G,COLMAN B.The effects of pH and dissolved inorganic carbon on external carbonic anhydrase activity in Chlorella saccharophila[J].Plant,Cell and Environment,1996, 19(4):485-489.
[12] 刘洪霞,孙颖颖,王长海.pH和氮素对球等鞭金藻胞外碳酸酐酶活性的影响[J].烟台大学学报(自然科学与工程版),2008,21(4):286-290.
[13] 刘春光,金相灿,孙凌,等.pH对淡水藻类生长和种类变化的影响[J].农业环境科学学报,2005,24(2):294-298.
[14] 游亮,崔莉凤,刘载文,等.藻类生长过程中DO、pH与叶绿素相关性分析[J].环境科学与技术,2007,30(9):42-44.
[15] 李小龙,耿亚红,李夜光,等.从光合作用特性看铜绿微囊藻(Microcystis aeruginosa)的竞争优势[J].武汉植物学研究,2006, 24(3):225-230.
[16] 张宝玉,李夜光,李中奎,等.温度、光照强度和pH对雨生红球藻光合作用和生长速率的影响[J].海洋与湖沼,2003,34(5): 558-565.
[17] 许海,刘兆普,袁兰,等.pH对几种淡水藻类生长的影响[J].环境科学与技术,2009,32(1):27-30.
[18] 杨桂娟,栾忠奇,周笑辉.温度对小球藻生长量和溶氧量影响研究[J].农机化研究,2009(9):157-158,161.
[19] 杜宇,孙雪,徐年军.不同盐度和Fe³+浓度对小球藻长、硝酸还原酶活性及基因表达的影响[J].生态科学,2012,31(4): 441-445.
[20] RAI P K,MALLICK N,RAI L C.Effect of Cu and Ni on growth,mineral uptake,photosynthesis and enzyme activities of Chlorella vulgaris atdifferent pH values[J].Biomedical and Environmental Sciences,1994,7(1):56-67.
[21] ZHANG G Y,WEN X B,LIANG F,et al.The effect of physical and chemical factors on the growth and lipid production of Chlorella[J].Acta Ecologica Sinica,2011,31(8):2076-2085.
[22] 张虎,张桂艳,温小斌,等.pH对小球藻Chlorella sp.XQ-200419光合作用、生长和产油的影响[J].水生生物学报,2014(6):1000-3207.
[23] AHMAD I,HELLEBUST J A.Osmoregulation in the extremely euryhaline marine micro-alga Chlorella autotrophica[J].Plant Physiology,1984,74(4):1010-1015.
[24] JIN W.Effect of salt and Cr³+ on physilolgy and biochemistry of signal-cell alage[J].Journal of Hebei University (Natural Science Edition),2002,1:44-50.
[25] 刘加慧,杨洪帅,王辉.温度、盐度、pH对小球藻生长率的联合效应[J].水生生物报,2014,38(3):446-453.
[26] 朱晓艳,张丹,梁芳,等.环境因子对小球藻(Chlorella sp.XQ-20044)光合作用的影响[J].植物科学学报,2014,32(1):74-79.