Abstract: In order to ascertain the correlation of rice tillering ability between main and ratoon season， and aim to provide germplasm resourse available not only for breeding， but also researching on related genetic analysis and physiological mechanism of strong regenerative ability rice， a total of 247 rice entities were adopted in this study collected from International Rice Research Institute（IRRI）. The experiment was conducted in Jingzhou city of Hubei province， Lingshui Li autonomous county of Hainan provice， Yingde city of Guangdong provice in 2017. Maximum tiller and fertile tiller number per plant were investigaed during main and ratoon season. The correlation between four tillering phenotypes of main and ratoon season， tillering ability and ratoon characteristics under the different growing environments was analyzed. Results indicated that maximum tiller and fertile tiller number both in main and ratoon season demonstrated significant positive correlation under three growing environments. Tillering ability showed significantly different under three growing environments， it was observed as Hubei， Hainan and Guangdong in descending order. Average ratooning rate of both indica and japonica subgroup was more than 130% under Hubei and Hainan growing environment， and there was no significant difference in between， no obvious difference was found at the same growing environment. Ratooning rate of indica and japonica subgroup in Guangdong was significantly higher than that in Hubei and Hainan. Our results indicated that a material with extremely high or low ratooning rate at one growing environment is not reproducible under another growing environment.