Analysis on drug resistance phenotype of Escherichia coli isolated from different pigs cultivation modes in Guangxi

doi:10.14088/j.cnki.issn0439-8114.2020.01.025

Abstract

Abstract: This study was to investigate the difference of drug resistance phenotype of E. coli isolated from different pigs cultivation modes in guangxi, and to provide a reference for the scientific use of antimicrobial drugs of swine colibacillosis and to slow down the drug resistant strains. E. coli from different pigs cultivation modes in Guangxi was isolated and identified, and the drug-resistant phenotype was tested and analysis. The results showed that, the E.coli isolates from three pigs cultivation modes were highly sensitive to cefoxitin, ceftazidime and amikacin, the sensitive rate up to 70%, while highly resistant to penicillin G, ampicillin, amoxicillin, rifampicin, lincomycin and sulfamethoxazole, the resistance rate up to 90%. The resistance rate of E.coli from large scale pig farms against enrofloxacin was extremely significantly lower than free-range farming （P<0.01）, the resistance rate of E.coli from large scale pig farms against ofloxacin, gentamicin, florfenicol were significantly lower than free-range farmers （P<0.05）. The resistance rate of E. coli from small and medium-sized pig farms against enrofloxacin, doxycycline and florfenicol were significantly lower than free-range farmers （P<0.05）. The resistance rate of E. coli from free-range farmers against ciprofloxacin, kanamycin, spectinomycin were extremely significantly higher than large scale pig farms and small and medium-sized pig farms（P<0.01）, while the resistance rate against cefotaxime was extremely significantly lower than large scale pig farms and small and medium-sized pig farms（P<0.01）. The multiple antibiotics resistance index （MARI） of E. coli from large scale pig farms, small and medium-sized pig farms and free-range farmers was 0.62, 0.63 and 0.68. 14 to 23 resistant E. coli strains accounted in free-range farming, which was extremely significantly higher than large scale pig farms（P<0.01）, and was significantly higher than small and medium-sized pig farms（P<0.05）. The results indicated that the resistance of E. coli from different pigs cultivation mode were different but all very serious and mainly of multi-drug resistance.

Key words: cultivation mode, pig Escherichia coli, drug-resistant, Guangxi

CLC Number:

S858.28

FENG Shi-wen, LI Jun, PAN Yan, HE Hui-li, PENG Hao, ZHONG Shu-hong, HU Shuai, YANG Wei. Analysis on drug resistance phenotype of Escherichia coli isolated from different pigs cultivation modes in Guangxi[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 114-118.

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