Analysis of molecular characteristics and receptor-binding ability of low pathogenic and highly pathogenic H7N9 AIV subtype avian influenza virus

doi:10.14088/j.cnki.issn0439-8114.2020.07.044

Abstract

Abstract: Two H7N9 subtype avian influenza viruses （AIV） isolated from chicken pathological material in Hubei Province, A/chicken/Hubei/093/2017（H7N9） and A/chicken/Hubei/207/2017（H7N9）（with CK93 and CK207 as their abbreviation, respectively） were analyzed for whole-genome sequencing. The hemagglutinin （HA） and neuraminidase （NA） sequence conservation, as well as HA glycosylation site, NA resistance site and key amino acid sites of 6 internal gene-encoded proteins were analyzed, meanwhile, the receptor binding ability of the two strains was determined as well. The results suggested that the HA protein cleavage site of CK93 was PEIPKGR↓GLF, which indicated a low pathogenicity of CK93, whereas CK207 was mutated to PKPKRTAR↓GLF, which indicated a high pathogenicity of CK207. At the same time, the 226 amino acid sequence of HA in CK93 was Leucine （226L）, while its counterpart in CK207 was Glutamine （226Q）. However, the receptor binding ability results suggested that two isolates can be bound to both α-2,3 sialic acid receptor and α-2,6 sialic acid receptor, it is indicated that the Q226L is not a decisive factor affecting receptor binding characteristics. In light of the above information, it is suggested that precaution and control to the mutation of H7N9 subtype avian influenza should be enhanced in order to lower any risk of epidemic owing to its mutation.

Key words: H7N9 subtype avian influenza virus, molecular characterization analysis, receptor-binding specificities

CLC Number:

S852.65

XUE Yu-han, REN Zhu, SHANG Yu, TANG Guo-yi, JIA Miao-miao, LU Yue-feng, LI Qiong-qiong, LI Hui-min, LUO Qing-ping, WEN Guo-yuan, PAN Zi-shu, ZHAO Zong-zheng. Analysis of molecular characteristics and receptor-binding ability of low pathogenic and highly pathogenic H7N9 AIV subtype avian influenza virus[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(7): 212-217.

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