Study on the abundance of VP7 protein of Bluetongue virus expressed by recombinant Baculovirus under two culture conditions

doi:10.14088/j.cnki.issn0439-8114.2022.23.022

HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (23): 117-120.doi: 10.14088/j.cnki.issn0439-8114.2022.23.022

• Animal Husbandry & Veterinary Medicine • Previous Articles Next Articles

Study on the abundance of VP7 protein of Bluetongue virus expressed by recombinant Baculovirus under two culture conditions

CHEN Chao-lin¹, HAN Dian-gang², YANG Yun-qing², ZHANG Chong², LI Jing², LUO Qian-min², YIN Shang-lian², DONG Xian-lan², LI Ling-feng², SHI Ya-ling¹, AI Jun², XIN Ji-ge¹

1. College of Veterinary Medicine,Yunnan Agricultural University, Kunming 650201, China;
2. Kunming Customs District P. R. China, Kunming 650228, China

Received:2021-04-11 Online:2022-12-10 Published:2023-01-27

Abstract

Abstract: In order to obtain Bluetongue virus （BTV） VP7 protein with high abundance expression on the recombinant Baculovirus, the recombinant Baculovirus was cultured by cell attachment culture and shake flask culture. After 5 days of culture, the culture medium was collected every day. The expression of BTV VP7 protein was detected by ELISA, and the data were statistically analyzed by SPSS software. The results showed that the expression of BTV VP7 protein in adherent culture was significantly higher than that in shake flask culture. The expression of BTV VP7 protein in both culture conditions was the highest on the third day, and then began to decline. The results showed that the Baculovirus insect cell system was more suitable for large-scale expression of BTV VP7 protein by adherent culture.

Key words: Sf9 insect cells, Bluetongue virus VP7 protein, adherent culture, shaking culture

CLC Number:

S852.65

CHEN Chao-lin, HAN Dian-gang, YANG Yun-qing, ZHANG Chong, LI Jing, LUO Qian-min, YIN Shang-lian, DONG Xian-lan, LI Ling-feng, SHI Ya-ling, AI Jun, XIN Ji-ge. Study on the abundance of VP7 protein of Bluetongue virus expressed by recombinant Baculovirus under two culture conditions[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(23): 117-120.

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Study on the abundance of VP7 protein of Bluetongue virus expressed by recombinant Baculovirus under two culture conditions

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