Determination of total iridoid glutinoside content of Rehmannia glutinosa by Sulfo-Phospho-Vanillin method

doi:10.14088/j.cnki.issn0439-8114.2021.05.022

Abstract

Abstract: To establish a method for determination of total iridoid glutinoside in the extract of Rehmannia glutinosa and measure the content of total iridoid glutinoside in seven Rehmannia glutinosa germplasms. The Sulfo-Phospho-Vanillin method was applied to compare the content of total iridoid glutinoside in Rehmannia glutinosa. The absorbance was detected at 510 nm. The results showed that a stand curve using catalpol was constructed at a concentration rang of 0.01~0.25 mg/mL. The regression equation was A=1.191 7ρ-0.003 5，the correlation coefficient is 0.999 9. It showed that the content of total iridoid glutinosides in leaves was usually higher than that in roots. The method established in this study is table， simple and rapid for determination of the total iridoid content in Rehmannia glutinosa，and it will provide convenience for content evaluation studies on samples of Rehmannia glutinosa.

Key words: Rehmannia glutionosa, iridoid glutinoside, germplasm

CLC Number:

R284.2

SUN Peng, MA Jun-zheng, DUAN Chen-chen, FU Na-na, HUANG Yong, LI Xian-en. Determination of total iridoid glutinoside content of Rehmannia glutinosa by Sulfo-Phospho-Vanillin method[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(5): 115-117.

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