HS-SPME-GC-MS analysis of volatile components in ebony plum and processed products

doi:10.14088/j.cnki.issn0439-8114.2021.09.022

Abstract

Abstract: Use headspace solid phase microextraction （HS-SPME） and gas chromatography-mass spectrometry （GC-MS） to analyze and identify the volatile components of ebony plum, vinegar ebony plum and ebony charcoal, normalized by area Method to calculate the relative percentage of each component. The results showed that a total of 46 components were detected, 34 components were identified, 32 components were detected from ebony plum, 23 components were identified, accounting for 88.90% of the volatile components. Eighteen components were detected from vinegared ebony plum, and 14 components were identified, accounting for 82.52% of the total volatile components. 28 components and 18 components were identified from ebony charcoal, accounting for 91.93% of the total volatile components. There are 9 kinds of volatile components in the three. Compared with raw ebony products, vinegar-based ebony ume has 4 new components and 13 components are reduced, and ebony charcoal has 8 new components and 13 components. The composition and content of volatile components of ebony plum has changed significantly after vinegar preparation and charcoal frying, and the pharmacological effects are different. This method is stable and reliable, and is suitable for the rapid analysis of volatile components of ebony plum.

Key words: ebony plum, vinegar ebony plum, ebony charcoal, HS-SPME-GC-MS, volatile components

CLC Number:

LI Shu-fan, LIU Yi, JIANG Han-mei, LIU Tian-qi. HS-SPME-GC-MS analysis of volatile components in ebony plum and processed products[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 113-116.

References

[1] 国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015.79.
[2] 张小琼,侯晓军,杨敏,等.乌梅的药理作用研究进展[J].中国药房,2016,27(25):3567-3570.
[3] 张华月,李琦,付晓伶.乌梅化学成分及药理作用研究进展[J].上海中医药杂志,2017,51(S1):296-300.
[4] 吴仁海,刘红彦,尹新明,等.菊花茎叶挥发油的GC-MS分析[J].河南中医学院学报,2008(5):26-27.
[5] 胡文杰,许樟润,鲁思情,等.皇菊花发育过程中挥发油化学成分及其抗氧化活性的研究[J].植物研究,2016,36(6):942-948.
[6] 刘晓晓. 禾本科植物中苜蓿素含量测定及HSCCC快速高效制备方法的建立[D].山东青岛:青岛大学,2019.
[7] 昌星. 工业双戊烯合成异松油烯的研究[D].长沙:中南林业科技大学,2018.
[8] 刁银军,许玲玲,麻佳蕾,等.GC-MS联用分析三个品种杨梅树叶的挥发油组分[J].化学分析计量,2009,18(1):25-28.
[9] 王江宽. 控制DO和SRT条件下短程硝化反硝化及系统稳定性研究[D]. 山东青岛:青岛大学,2011.
[10] 沈小钟,崔穗旭,邱蔚芬,等.GC法同时测定跌打镇痛膏中樟脑、薄荷脑和水杨酸甲酯含量[J].中国药师,2020,23(4):772-774.
[11] 游洁. 杏仁的研究概况[J].中国医药指南,2014,12(33):57-58.
[12] 李兰. 苯甲醇质量分析研究及整体质量评价[D].长沙:中南大学,2014.
[13] 陈莉萍,吴连堤,邱秀梅.桃红四物汤联合低分子肝素钠防止老年股骨转子间骨折全髋置换术后深静脉血栓效果观察[J].江西中医药,2020,51(9):38-41.
[14] 刘腾飞. 短链脂肪酸和乙酸乙酯抗炎作用及其机制研究[D].河北保定:河北大学,2013.
[15] 李其书,范怀焰,石必凤,等.董酒中健康价值生物活性物质的探讨(四)[J].酿酒科技,2020(9):119-123.
[16] 刘安洲,杜金华,王晓.泰山蜂胶挥发性成分检测[J].食品与发酵工业,2009,35(5):163-166.
[17] 张利亚,刘霞,张佩.小麦秸秆木醋液成分及抑菌效果研究[J].江苏农业科学,2020,48(18):219-222.
[18] 陈峥,刘波,陈梅春,等.基于顶空-固相微萃取-气质联用的短芽胞杆菌挥发性抑菌代谢物的鉴定[J].食品安全质量检测学报,2014,5(9):2844-2852.
[19] 刘琪铭. 环糊精改性二氧化钛材料对水中BPA等污染物的光催化去除[D].上海:华东理工大学,2020.
[20] MARONGIU B,PIRAS A,PORCEDDA S,et al.Chemical composition of the essential oil and supercritical CO₂ extract of Commiphora myrrha (Nees) Engl. and of Acorus calamus L.[J].Journal of agricultural and food chemistry,2005,53(20):7939-7943.