Research progress of CRISPR-Cas9 technology application and achievement in vegetable crops genetic improvement

doi:10.14088/j.cnki.issn0439-8114.2022.24.022

Abstract

Abstract: In plants, CRISPR-Cas genome editing technology is a new technique to alter gene functions to generate improved crop varieties. Compared with traditional breeding, CRISPR-Cas is a simple, low-cost, efficient and accurate technology with a lower risk of off-target effects. Vegetable crops are rich in dietary fiber, vitamins and minerals, which are essential for human health. However, with the change of climate and environment, biotic and abiotic stresses pose a serious threat to vegetable production, affecting the quality and yield. In this review, the history of CRISPR technology, the components of CRISPR-CAS toolbox, the variants of existing Cas proteins, the methods of transforming CRISPR-CAS elements into plants, and the specific cases of gene editing systems in the genetic improvement of vegetable crops were summarized. Finally, the challenges and application prospects of CRISPR-Cas technology in vegetable breeding were put forward.

Key words: CRISPR-Cas9, vegetable, double-strand breaks（DSB）, DNA repair, precision editing, trait improvement

CLC Number:

S330
S336

WAN Li-li, WANG Zhuan-rong, TANG Mi, ZHANG Xue-jun, ZENG Hong-xia, REN Jian, ZHANG Na, SUN Yu-hong, XIONG Jian-shun, ZHU Zhi-kun. Research progress of CRISPR-Cas9 technology application and achievement in vegetable crops genetic improvement[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 100-108.

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