Optimization of extraction process and biological activity analysis of total oligosaccharides from Cistanche deserticola

doi:10.14088/j.cnki.issn0439-8114.2025.02.024

Abstract

Abstract: The total oligosaccharides from Cistanche deserticola were extracted by ultrasonic-assisted method, and the extraction condition was optimized by single factor and orthogonal test. The structural characteristics of total oligosaccharides of Cistanche deserticola were preliminarily analyzed by infrared spectroscopy. The in vitro hypoglycemic ability and antioxidant activity of total oligosaccharides from Cistanche deserticola were studied by measuring the inhibition ability of α-glucosidase, and the scavenging rate of DPPH, ·OH and ABTS free radicals. The results showed that the optimum extraction condition of total oligosaccharides from Cistanche deserticola was the solid-liquid ratio of 1∶50, ethanol concentration of 40% and ultrasonic time of 32 min. Under this condition, the yield of total oligosaccharides was 17.32%. The infrared spectroscopy showed that there was obvious characteristic absorption of total oligosaccharides, and it was β-pyranose. The results of biological activity showed that the inhibition rate of α-glucosidase was 63.94%, and the scavenging rates of DPPH, ·OH and ABTS free radicals were 85.63%, 60.91% and 82.90%, respectively. This method was helpful to improve the yield of total oligosaccharides from Cistanche deserticola, and these total oligosaccharides had strong antioxidant and hypoglycemic ability in vitro, which could provide reference for related research.

Key words: Cistanche deserticola, total oligosaccharides, ultrasonic assisted extraction, antioxidation, hypoglycemic

CLC Number:

R284.2

TIAN Zhan-ping, WANG Yu, JI Shu-yi, TAN Jun-jie, YAO Jun, SHEN Jing. Optimization of extraction process and biological activity analysis of total oligosaccharides from Cistanche deserticola[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 156-161.

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