Regulatory effects of carbon source and different concentrations of salicylic acid on the physiological characteristics and starch accumulation in Spirodela polyrrhiza

doi:10.14088/j.cnki.issn0439-8114.2025.09.022

Abstract

Abstract: The regulatory effects of a carbon source and salicylic acid (SA) on the physiological characteristics and starch accumulation in Spirodela polyrrhiza were investigated. Under conditions with or without a carbon source,Spirodela polyrrhiza was treated with different concentrations of SA, and related physiological and biochemical indexes were determined. The results showed that both the dry and fresh weights of Spirodela polyrrhiza decreased with increasing SA concentration. However, the presence of a carbon source alleviated this growth inhibition. Chlorophyll fluorescence kinetics analysis indicated that the dissipated energy flux per reaction center (DIo/RC) increased, while the electron transport flux per reaction center (ETo/RC), the photosynthetic performance index (PIabs), and the comprehensive performance index (PItotal) decreased. The presence of a carbon source mitigated these changes, thereby reducing the inhibitory effect of SA on photosynthetic efficiency. Catalase (CAT) activity showed an upward trend with the increase of SA concentration only in the presence of a carbon source. Peroxidase (POD) activity showed an increasing trend, with a more pronounced increase in the absence of a carbon source. Superoxide dismutase (SOD) activity increased only in the presence of a carbon source, peaking at 200 μmol/L SA. In the presence of a carbon source, the malondialdehyde (MDA) content first increased and then decreased, reaching its lowest level at 200 μmol/L SA. Under carbon source starvation, the MDA content accumulated continuously. Starch content increased with increasing SA concentration and was higher in the presence of a carbon source.

Key words: carbon source, salicylic acid, Spirodela polyrrhiza, physiological characteristics, starch accumulation

CLC Number:

Q945.79

HUANG Ting, JIA Rong, XIANG Bei-bei. Regulatory effects of carbon source and different concentrations of salicylic acid on the physiological characteristics and starch accumulation in Spirodela polyrrhiza[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 134-140.

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