Application of Bacillus velezensis EA19 to substitute part of pydiflumetofen in the control of Fusarium head blight

doi:10.14088/j.cnki.issn0439-8114.2026.01.012

Abstract

Abstract: The efficacy of combining the biocontrol agent Bacillus velezensis EA19 with the succinate dehydrogenase inhibitor (SDHI) fungicide pydiflumetofen (Pyd) against Fusarium head blight (FHB) in wheat was evaluated through an integration of bioinformatics analysis, in vitro bioassays, and field efficacy trials. Bioinformatics analysis revealed that strain EA19 possessed two SDH components (flavoprotein FP and iron-sulfur protein IP), which were phylogenetically distant from their homologs in Fusarium spp., and lacked the cytochrome b (Cytb) protein target for Pyd. In vitro compatibility tests showed that Pyd concentrations ranging from 0.1 to 12.5 μg/mL had no significant inhibitory effect on EA19 growth. The EC₅₀ of EA19 fermentation broth powder and Pyd against Fusarium graminearum PH-1 were 19.65 g/L and 0.12 μg/mL, respectively. At their respective EC₅₀ doses, co-application of the two agents at solution volume ratios of 6︰4 and 3︰7 (EA19︰Pyd) yielded the strongest synergistic inhibition of PH-1 mycelial growth, with toxicity ratios of 1.48 for both combinations, indicating significant synergism. Field trials demonstrated that combining a 60% or 30% reduced dose of Pyd suspension concentrate (SC) with EA19 wettable powder (WP) at the aforementioned ratios achieved control efficacy statistically equivalent to the full dose of Pyd SC alone. However, the efficacy in reducing deoxynivalenol (DON) mycotoxin content was significantly lower than that of the full Pyd SC treatment. Both combination treatments resulted in wheat grain yield and thousand-kernel weight comparable to the full Pyd SC treatment. Notably, the treatment combining a 30% reduced Pyd SC dose with EA19 WP showed a tendency to increase thousand-kernel weight.

Key words: Fusarium head blight, Bacillus velezensis, pydiflumetofen, combination, control efficacy

CLC Number:

WANG Wen-xiao, CHEN Ting-ting, QUE Ya-wei, XUE Min-feng, NI Hao-xiang, DENG Chun-lin, YANG Li-jun, LI Li, GONG Shuang-jun, ZHOU Hua-zhong, ZENG Fan-song. Application of Bacillus velezensis EA19 to substitute part of pydiflumetofen in the control of Fusarium head blight[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 66-72.

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