The biocontrol efficacy of two Bacillus strains against tobacco bacterial wilt

doi:10.14088/j.cnki.issn0439-8114.2026.02.014

Abstract

Abstract: To evaluate the biocontrol efficacy of Bacillus atrophaeus strain BS17 and Bacillus velezensis strain BS22 against tobacco bacterial wilt and determine the optimal application timing, LIVE/DEAD fluorescence staining and scanning electron microscopy were employed to observe the inhibitory effects of these two strains on Ralstonia solanacearum (the causal agent of bacterial wilt) and analyze their inhibitory mechanisms. Additionally, pot experiments were conducted with bacterial suspensions applied at three key growth stages: Sowing stage, seedling emergence stage, and transplanting stage. The results demonstrated that both strains primarily inhibit Ralstonia solanacearum by disrupting the integrity of the bacterial cell membrane. The BS22 strain exhibited superior control efficacy when applied at the sowing stage (98.23%), significantly higher than that of BS17 (79.64%, P<0.001). When applied at the seedling emergence stage, no significant difference in control efficacy was observed between the two strains (BS17: 88.49%, BS22: 85.84%). However, when applied at the transplanting stage, both strains exhibited significantly reduced control efficacy (BS17: 3.54%, BS22: 48.67%). The BS22 strain demonstrated superior biocontrol efficacy against tobacco bacterial wilt compared to the BS17 strain, particularly when applied at the sowing stage.

Key words: Bacillus atrophaeus strain BS17, Bacillus velezensis strain BS22, tobacco bacterial wilt, control efficacy, application timing

CLC Number:

CHENG Wei, WANG Liao-yuan, HUO Guang, KONG Wei, SHI He-li, XIANG Bi-kun, ZHANG Ran, DONG Rui-jie, YANG Zhao-yu, CAO Yuan-yuan, TAN Jun, WANG Rui. The biocontrol efficacy of two Bacillus strains against tobacco bacterial wilt[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 95-100.

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