Cloning and functional verification of cucumber CsAuxin-15A under saline-alkali stress

doi:10.14088/j.cnki.issn0439-8114.2026.06.036

Abstract

Abstract: To investigate the biological function of the SAUR（Small auxin up-regulated RNA）family gene CsAuxin-15A in cucumber（Cucumis sativus L.）in response to saline-alkali stress, the CsAuxin-15A gene was cloned from the saline-alkali tolerant cucumber line D1909 and the saline-alkali sensitive line D1604, bioinformatics analysis, subcellular localization, prediction of cis-acting elements in the promoter and expression pattern analysis were performed, sense and antisense expression vectors were simultaneously constructed to transform cucumber, and the peroxidase（POD）activity and proline（Pro）content of transgenic plants under saline-alkali stress were measured. The results showed that the full-length of CsAuxin-15A was 303 bp, encoding 100 amino acids, belonged to the SAUR-type auxin-responsive protein family, and was localized in the cytoplasm; its promoter region contained multiple cis-acting elements such as light-responsive, abscisic acid-responsive, defense and stress-responsive, ARE anaerobic induction, and MYB/MYC binding sites. Under saline-alkali stress, the relative expression of CsAuxin-15A in stems, leaves and fruits of D1909 was significantly higher than that in the control, with the highest expression in leaves, while no significant difference was observed in roots. Compared with wild-type plants, the CsAuxin-15A sense-transgenic plants grew better under saline-alkali stress, and the leaf peroxidase activity and proline content were significantly higher than those in the control. In conclusion, CsAuxin-15A enhanced the tolerance of cucumber to saline-alkali stress by positively regulating the antioxidant enzyme activity and the accumulation of osmotic adjustment substances.

Key words: Cucumis sativus L., CsAuxin-15A, cloning, saline-alkali stress, functional verification

CLC Number:

S642.2

YANG Yang, ZHANG Jun-ming, YU Cong-rui, ZHANG Ji-hao, LUO Tian-you, ZHANG Fan. Cloning and functional verification of cucumber CsAuxin-15A under saline-alkali stress[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(6): 234-245.

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