Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415

doi:10.14088/j.cnki.issn0439-8114.2018.22.018

HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (22): 64-66.doi: 10.14088/j.cnki.issn0439-8114.2018.22.018

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Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415

QIAN Chun-guo¹, ZHOU Yi-quan², YANG Xi-liang¹, LI Kui³, SHI Li-qiao³

1.Department of Pharmacy,School of Medicine,Wuhan University of Science and Technology,Wuhan 430065,China;
2.Xiantao Agricultural Bureau,Xiantao 433000,Hubei,China;
3.Hubei Biopesticide Engineering Research Center,Wuhan 430064,China

Received:2018-08-29 Online:2018-11-25 Published:2019-12-19

Abstract

Abstract: Sponge epiphytic fungi FWZ0415 was cultured in solid state fermentation,and its secondary metabolites were isolated,purified and identified. Using silica gel column chromatography,Sephadex column（Sephadex LH-20） and the preparation of semi preparative high performance liquid chromatography（HPLC） methodtwo compounds were purified,and then identified as violaceol-I and violaceol-II by nuclear magnetic resonance 1H-NMR and 13C-NMR. The cytotoxicity of two compounds to K252,MCF-7 and SGC7901 were tested,violaceol-I was not significantly cytotoxicity（IC₅₀>30 μmol）. The IC₅₀ of violaceol-II to three tumor cell lines were 16.7,16.3,15.8 μmol,respectively.

Key words: spongye epiphytic fungi, secondary metabolites, isolation and purification, structural identification, antitumor activity

CLC Number:

R284
R285

QIAN Chun-guo, ZHOU Yi-quan, YANG Xi-liang, LI Kui, SHI Li-qiao. Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(22): 64-66.

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Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415

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