Study on Synergistic Antioxidant Activity of 4 Kinds of Edible Fungi Compound Polysaccharide in Vitro

doi:10.14088/j.cnki.issn0439-8114.2018.03.018

Abstract

Abstract: Edible fungi [Hericium erinaceus （Bull.） Per.,Pleurotus eryngii Quel.,Lentinus edodes（Berk.） Sing.,Pleurotus ostreatus]were fermented to obtain mycelium. Polysaccharide were extracted from the mycelium and determined the DPPH free radical scavenging and reducing power of the four polysaccharides. Four kinds of polysaccharide were mixed in different proportions（respectively programme 1∶Hericium erinaceus（Bull.） Per.∶Pleurotus ostreatus∶Lentinus edodes（Berk.） Sing.∶Pleurotus eryngii Quel.=1∶1∶1∶1,programme 2∶Hericium erinaceus（Bull.） Per.∶Pleurotus ostreatus∶Lentinus edodes（Berk.） Sing.∶Pleurotus eryngii Quel.=4∶3∶2∶1,programme 3∶Hericium erinaceus（Bull.） Per.∶Pleurotus ostreatus∶Lentinus edodes（Berk.） Sing.∶Pleurotus eryngii Quel.=5∶4∶3∶2）,and study the antioxidant activity of compound polysaccharide. The results showed that the four kinds of mycelial polysaccharides have certain reduction capacity and the ability of scavenging DPPH free radical. Among them,the antioxidant activity of Hericium erinaceus polysaccharide was the highest. The results showed that the antioxidant activity from programme 2 was the best, and was significantly higher than that of single polysaccharide.

Key words: edible fungi, compound polysaccharide, antioxidant, activity

CLC Number:

S646

ZHANG Zhi-chao, WU Mi, TIAN Xiao, XU Yan. Study on Synergistic Antioxidant Activity of 4 Kinds of Edible Fungi Compound Polysaccharide in Vitro[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(3): 78-80.

References

[1] KOH J H,YU K W,SUH H J,et al.Activation of macrophages and the intestinal immune system by an orally administered decoction from cultured mycelia of Cordyceps sinensis[J].Bioscience,Biotechnology,and Biochemistry,2002,66(2):407-411.
[2] 方一泓,余萍,李薇,等.茶树菇子实体多糖的分离纯化和免疫活性研究[J].食用菌学报,2007,13(4):63-73.
[3] YEN G C,WU J Y.Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae[J].Food Chemistry,1999, 65(3):375-379.
[4] 陈炼红,杨丽珠,索化夷,等.响应面法优化松茸多糖酶法提取工艺及其体外抗氧化性分析[J].食品科学,2014,35(16):23-28.
[5] WASSER S.Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J].Applied Microbiology and Biotechnology,2002,60(3):258-274.
[6] ZHANG Y,GU M,WANG K P,et al.Structure,chain conformation and antitumor activity of a novel polysaccharide from Lentinus edodes[J].Fitoterapia,2010,81(8):1163-1170.
[7] 王峰,陶明煊,程光宇,等.4种食用菌提取物自由基清除作用及降血糖作用的研究[J].食品科学,2009,30(21):343-347.
[8] 杜梅,张松.食用菌多糖降血糖机理研究[J].微生物学杂志,2007,27(2):83-87.
[9] 余志坚,陈传红,赵晋宇.DNS法检测食用菌多糖含量条件优化研究[J].江苏农业科学,2012,40(1):259-260.
[10] 张唐伟,杨乐,柳青海,等.地木耳多糖的抗氧化性与抑菌作用[J].食品与生物技术学报,2011,30(6):868-872.
[11] 李志涛,赵娟娟,王敏.复合食用菌多糖的免疫活性研究[J].食品研究与开发,2017,38(5):39-42.
[12] 陆武祥,王东华,王秀英,等.5种食用菌液体发酵菌丝抗氧化活性分析比较[J].食品与发酵工业,2013,39(7):124-127.
[13] 李丹,李晓磊,李荣和.玉米和大豆短肽的自由基清除活性与还原力的对比研究[J].食品工业科技,2008,29(8):71-73.

[1]	DAI Lu, ZHANG Zi-yi, XUE Wen, SHEN Jing, TANG Li-li, LONG Ming-hua. Preparation and properties of polylactic acid/oregano essential oil antioxidant active packaging film [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 110-112.
[2]	YANG Jin-hong, CHEN Zheng-yu, MENG Gang, KONG Wei-qing. Determination of functional components and antioxidant activities of mulberry juice and mulberry wine [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 117-119.
[3]	DAI Yuan, LENG Jin-song, LU Hong-jia. Study on process optimization for the extraction of pea protein and its emulsification and foaming activity [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(8): 134-139.
[4]	SONG Dan, HAN Lu, YANG Hong, NA Xi-xue, NING Xiao-qing, QIN Guo-le. Survey on edible（medicinal） macrofungi resources in Jinxiu estuary protection station [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(7): 95-98.
[5]	CHEN Zi-ting, TANG Shi-miao, TAN Jiang-ying, WU Qian. Interaction between catechin and chitosan oligosaccharide with superior synergistic antibacterial activity [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(7): 113-115.
[6]	GOU Wen-bo, YANG Zhong-duo, ZHOU Ge-ge. Isolation and anti-monoamine oxidase activity of endophytic fungi from four medicinal plants [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 56-59.
[7]	YAN Dan, CAI Yan-qu, LI Tie-cheng, CHEN Bi-tian, FENG Hua-zi, ZHANG Zhi-peng. Optimization extraction through Box-Behnken response surface methodology and antioxidant activity in vitro of polysaccharides from aerospace hybrid Broussonetia papyrifera leaves [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 100-105.
[8]	JI Ruo-wei, YANG Yang, LIN Peng-cheng. Chemical composition and antimicrobial activity of the essential oil from Rhododendron capitatum leaves [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 120-124.
[9]	CHANG Yue, LI Jun-kai, CHEN Shun-shun, XU Han-hong. Synthesis,biological activity and translocation of L-Glu-amicarthiazol conjugate in tobacco plants [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(10): 67-71.
[10]	CHEN Rui-ling, SHI Zheng, LI Jie, QU Kai-yue, XU Jia-lun. Three-dimensional numerical simulation of lightning strike buildings group [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(1): 141-146.
[11]	WANG Jie, XIA Cai-fen, ZHOU Li, TIAN Xiang. Preparation and antioxidant evaluation on hardleaf oatchestnut starch/carboxymethyl chitosan blended-membrane [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(8): 116-120.
[12]	DONG Mei. Study on quality cost accounting of agricultural enterprises based on Activity-Based Costing：Taking JH company as an example [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(8): 189-193.
[13]	LI Jian, QIAO Wei-hua, WANG Tao, AO Yan-song. Effects of vegetable waste compost on soil fertility and enzyme activity [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(7): 59-63.
[14]	FU Hao, LIU Ping-ping, ZHU Jian-feng, LI Yan-long, LI Meng, WANG Chang-tao. Isolation and purification of yeast polypeptides and efficacy of cosmetics [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(7): 184-187.
[15]	LI Hong-chen, ZHU Shun-cheng. Analysis of amino acid metabolism in tobacco leaves among different varieties [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(5): 88-91.