Studies on the properties and antibacterial mechanism of crude lipopeptide extracts from Aspergillus flavus antagonistic bacterium Bacillus Amyloliquefaciens B10-6-1

doi:10.14088/j.cnki.issn0439-8114.2019.14.017

Abstract

Abstract: The crude lipopeptide extract was extracted from the fermentation broth of strain B10-6-1 by acid precipitation. The minimum inhibitory concentration and the antimicrobial spectrum were determined by the inhibition circle method. The effects of temperature, pH, protease, metal ions, EDTA and other factors on the bacteriostatic activity were investigated. The effects of crude lipopeptide extracts on Aspergillus flavus spore germination and hyphae were investigated by spore germination and microscopic observation. The results showed that the minimum inhibitory concentration of crude lipopeptide extract was 4 g/L, with the highest inhibitory activity at 40 ℃ and pH of 8, while temperature, pH, protease, metal ion and EDTA had no obvious effect on the antibacterial activity of crude lipopeptide extract. The antifungal spectrum of the crude extract was extensive and had obvious inhibitory effect on 9 strains of fungi such as Alternaria brassicae and Rhizoctonia cerealis etc. The mycelium treated with lipopeptide extract could dissolve the site of mycelium cell wall and decrease the germination rate of spores, which could inhibit the germination of Aspergillus flavus spores and the growth of hyphae.

Key words: Aspergillus flavus antagonistic bacteria, crude extract of lipopeptide antibiotics, characterization, antibacterial mechanism

CLC Number:

S476

ZHANG Xue-wen, WANG Yi-ding, LI Hong-ya, WANG Shu-xiang, WANG Quan, LI Shu-na. Studies on the properties and antibacterial mechanism of crude lipopeptide extracts from Aspergillus flavus antagonistic bacterium Bacillus Amyloliquefaciens B10-6-1[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(14): 70-75.

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