Establishment of high-efficiency propagation system using sterile seedling leaves in Lycium ruthenicum Murr.

doi:10.14088/j.cnki.issn0439-8114.2021.09.028

Abstract

Abstract: In present experiment, based on the measurement of anthocyanins content in single dried berry, the aseptic leaves derived from the sterile seedlings of L.ruthenicum Murr. which germinated on MS medium were used as explants, and optimization condition of hormone concentration and C source combination was selected for response frequency, callus inducing frequency and plantlet forming frequency using the response surface design Design-Expert software. The results showed that the best condition for response frequency and callus induction frequency was 6-BA 0.92 mg/L, NAA 0.45 mg/L, and C source for sucrose 2.26%, respectively; And best condition for plantlet formation frequency was 6-BA 1.15 mg/L, NAA 0.38 mg/L, and C source sucrose 2.93%, respectively. Result also showed that there was a strong interaction between the carbon source combination and hormones during the callus induction process. Tissue sections showed that the the way for plantlet forming induction or shoot regeneration adopted in the experiment may be the somatic embryogenesis, and RAPD test of the offspring regeneration obtained in present showed extremely low rate of variation which suggested the excellent characteristic of mother plant would maintain in descendant derived from this propagation system.

Key words: Lycium ruthenicum Murr., somatic embryogenesis regeneration, one step for plantlet formation, response surface analysis, rooting

CLC Number:

S888.2

HE Feng-ming, GUO Jia-yuan, SHEN Ting, MA Guan-xue, YAN Sheng-qi, ZHANG Yun-feng. Establishment of high-efficiency propagation system using sterile seedling leaves in Lycium ruthenicum Murr.[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 135-140.

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