Abstract: In response to the problems of incomplete detection technology and unreliable detection results for Lasioderma serricorne in Nicotiana tabacum L.,based on the results of electronic nose detection, the data results were analyzed using principal component analysis （PCA） and loading analysis. The results showed that the main volatile substances of the four cut tobacco were sulfur compounds, short-chain alkanes, alcohol ether aldehydes and ketones, small molecule nitrogen oxides, etc. The main volatile substances of insect like and sex hormones were small molecule nitrogen oxides and sulfides.After being damaged by insects, the sulfur compounds in cut tobacco decreased significantly. In the sensor（W1W） sensitive to inorganic sulfides, the response value of strong aroma insect cut tobacco decreased by 48.33% compared to strong aroma cut tobacco, and light aroma insect cut tobacco decreased by 59.48% compared to light aroma cut tobacco;in the sensor （W2W） sensitive to organic sulfides, the response value of the strong aroma pest cut tobacco decreased by 35.72% compared to the strong aroma cut tobacco, and the light aroma pest cut tobacco decreased by 48.55% compared to the light aroma cut tobacco. The PCA results showed that the four cut tobacco samples exhibited excellent discrimination, with almost no difference in principal component 1, which accounted for 99.70%, between female and male samples. The loading analysis results showed that the main odor component in the cut tobacco sample was sulfur compounds, while the main odor component in the tobacco beetle sample was small molecule nitrogen oxides, followed by sulfur compounds.

Key words: Nicotiana tabacum L., cut tobacco, pest infestation, Lasioderma serricorne, electronic nose, volatile components