Screening and identification of rhizobia from selenium-enriched soybean and their effects on growth and selenium accumulation of soybean

doi:10.14088/j.cnki.issn0439-8114.2025.04.011

Abstract

Abstract: Selenium-tolerant rhizobia were screened and identified from selenium-enriched soybean[Glycine max (Linn.) Merr.] nodules. Sodium selenate and sodium selenite were used as selenium sources, and different concentration gradients were set to study the selenium tolerance of rhizobia. Zhonghuang 301 was used as the test material, and the field experiment was set up with blank control (CK), rhizobium treatment (Rb), sodium selenite treatment (Se), rhizobium and sodium selenite combination treatment (Rb+Se) to study the effects of rhizobia combined with selenium on the growth and selenium accumulation of soybean. The results showed that 1.0 mg/L sodium selenite treatment had a certain promoting effect on the growth of rhizobia. Compared with CK, the plant height, main root length and dry weight per plant of soybean treated with Rb+Se increased by 78.67%, 82.98% and 69.57%, respectively, the selenium content of roots, stems, leaves, grains and nodules increased by 3.53,2.44,1.37,2.63 and 1.82 times, respectively, and the selenium content of soil increased by 2.10 times. Compared with Rb treatment, the plant height, main root length and dry weight per plant of soybean treated with Rb+Se increased by 21.45%, 32.31% and 14.71%, respectively, the selenium content of roots, stems, leaves, grains and nodules increased by 113.04%, 173.33%, 65.21%, 160.70% and 49.50%, respectively, and the selenium content of soil increased by 125.65%. Compared with Se treatment, the plant height, main root length and dry weight per plant of soybean treated with Rb+Se increased by 45.13%, 56.31% and 50.00%, respectively, the selenium content of roots, stems, leaves, grains and nodules increased by 30.14%, 50.01%, 27.96%, 22.59% and 18.19%, respectively, and the selenium content of soil increased by 19.23%. It could be seen that the combination of rhizobium and selenium was beneficial to the growth of soybeans, which could enhance the content of selenium in various parts of soybeans, and also increase the accumulation of selenium in cultivated soil.

Key words: selenium-enriched soybean[Glycine max (Linn.) Merr.], rhizobium, selenium tolerance, screening, identification, growth of soybean, selenium content

CLC Number:

S65.1
S182

HU Ye-qing, ZHANG Jie, HE Ting-ting, ZHANG Yue, LI Cong-hu, LIU Yun-shuang, HU Ting. Screening and identification of rhizobia from selenium-enriched soybean and their effects on growth and selenium accumulation of soybean[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(4): 58-63.

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