Effects of monoclonal antibody against early pregnancy factor on proliferation, migration and apoptosis of cervical cancer HeLa cells

doi:10.14088/j.cnki.issn0439-8114.2025.05.018

Abstract

Abstract: The inhibitory effect of monoclonal antibody against early pregnancy factor (EPF-B6) on the biological behavior of cervical cancer HeLa cells was investigated by studying the effects of EPF-B6 on the proliferation, migration, cloning and apoptosis of HeLa cells. RT-PCR and Western Blot were used to detect the expression of EPF gene in six common cancer cells and EPF protein in cell culture medium. The effects of different concentrations of EPF-B6 monoclonal antibody on the proliferation, migration and population dependence of HeLa cells were detected by CCK8 assay, cell scratch assay and plate cloning assay, respectively. The apoptosis and cycle of HeLa cells were detected by flow cytometry. Results showed that EPF gene was present in common tumor cells. EPF protein was expressed in the culture medium of cervical cancer HeLa cells, breast cancer MCF-7 cells and ovarian cancer A2780 cells. The results showed that EPF-B6 monoclonal antibody could significantly inhibit the proliferation, migration and colony formation of HeLa cells, it was concentration-dependent (P<0.01). The results of cell cycle analysis showed that EPF-B6 monoclonal antibody blocked the DNA replication process of HeLa cells at G2/M phase.

Key words: early pregnancy factors, monoclonal antibody, cervical cancer, Hela cells, proliferation, migration, apoptosis

CLC Number:

R711.74

DUAN Fu-chun, REN Hong-lin, HU Pan, LI Yan-song, LIU Xi-lin, WANG Han, LIU Meng-di, LI Hao-song, LU Shi-ying. Effects of monoclonal antibody against early pregnancy factor on proliferation, migration and apoptosis of cervical cancer HeLa cells[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 117-122.

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