Establishment and optimization of a one-step plantlet regeneration system for tissue culture of Artemisia annua L.

doi:10.14088/j.cnki.issn0439-8114.2026.06.035

Abstract

Abstract: To simplify the seedling production process of tissue culture of Artemisia annua L. and shorten the cultivation period, a one-step plantlet regeneration system for Artemisia annua L. was established. Using stem segments with axillary buds of Artemisia annua L. as experimental materials, a two-factor multilevel completely randomized design was adopted to optimize the concentration combinations of plant growth regulators at the stages of multiple shoot induction, proliferation, and rooting, and the effects of acclimatization duration on the survival rate and growth vigor of transplanted plantlets were investigated. The results showed that when tender stems with buds of Artemisia annua L. were used as explants, rinsed with running water for 30 min, and then sterilized with 0.1% mercuric chloride solution for 12 min, the survival rate of explants reached the highest level (86.67%), and the browning rate was maintained at a relatively low level. Using MS + 4 mg/L 6-BA as the induction medium, multiple shoot formation was directly induced, with a multiple shoot induction rate of 75.56% and the time for bud formation shortened to 9.67 d, which effectively promoted the one-step plantlet regeneration of Artemisia annua L. Using MS + 3 mg/L 6-BA as the proliferation medium for subculture, the proliferation coefficient reached 7.37, and the occurrence of vitrification was significantly delayed. The optimal rooting medium for test-tube plantlets was MS + 1 mg/L NAA, with a rooting rate of 97.78% and an average root number of 14.67 per plant. The optimal acclimatization protocol for transplanting tissue-cultured plantlets involved 2 days of closed-bottle acclimatization followed by 7 days of potted acclimatization with plastic film mulching, achieving a transplanting survival rate of 96.59%. This one-step plantlet regeneration system for Artemisia annua L. was characterized by a simple operational process and high propagation efficiency, which facilitated the large-scale production of Artemisia annua L. plantlets.

Key words: Artemisia annua L., tissue culture, one-step plantlet regeneration, technical system, rooting culture, acclimatization and transplanting

CLC Number:

S567.21⁺9

YANG Sheng-fei, ZHANG Mei, PENG Yi, LUO Hui-ling, PENG Xue-dong, CAI Shi-yun, DING Yuan-jie. Establishment and optimization of a one-step plantlet regeneration system for tissue culture of Artemisia annua L.[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(6): 227-233.

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