Research on tissue culture and plantlet regeneration of Iris germanica ‘FRANS HALS’

doi:10.14088/j.cnki.issn0439-8114.2019.23.055

Abstract

Abstract: Using the leaves and bulbs of Iris germanica 'FRANS HALS' as explants, orthogonal experiment was used to study the effects of different hormones and different concentration combinations on the induction of callus, adventitious bud differentiation and rooting of test-tube seedlings to provide an optimized method for rapid propagation, preservation and utilization of germplasm resources of the iris. The results showed that the sterilization time required for different explants was different. The leaves were 3% sodium hypochlorite for 3 min and the bulbs were 3% sodium hypochlorite for 5 min. The most suitable explants for appendix tissue culture were bulbs. The optimum medium for callus induction and bud differentiation of Iris was MS+NAA 0.10 mg/L+6-BA 1.00 mg/L; rooting medium was MS+1\2MS+NAA 0.10 mg/L; the substrate was peat∶ perlite=8∶2, and the survival rate was over 90%.

Key words: Iris germanica. L, tissue culture, explant, plantlet regeneration

CLC Number:

Q949.71⁺8.28

WU Hai-feng, LYV Yuan-da, ZHENG Hai-xia, ZHAO Ming-jing, WANG Jia-qing. Research on tissue culture and plantlet regeneration of Iris germanica ‘FRANS HALS’[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(23): 223-226.

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