防城金花茶SSR遗传多样性分析

doi:10.14088/j.cnki.issn0439-8114.2026.01.017

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (1): 97-104.doi: 10.14088/j.cnki.issn0439-8114.2026.01.017

防城金花茶SSR遗传多样性分析

李双双¹, 高慧¹, 苏梦雪¹, 陈锦媚¹, 谢蕾卉^2,3, 黄永光^2,3, 林兴³, 邓家刚¹, 谢阳姣¹

1.广西中医药大学,南宁 530200;
2.防城港市老科学技术工作者协会,广西防城港 538021;
3.防城港市中医医院,广西防城港 538021

收稿日期:2025-07-24 出版日期:2026-01-25 发布日期:2026-02-10
通讯作者: 谢阳姣,研究员,博士,主要从事中药、民族药资源开发与利用研究,(电子信箱)xieyangjiao@163.com。
作者简介:李双双(2000-),女,广西南宁人,在读硕士研究生,研究方向为中药资源鉴定、评价与开发,(电子信箱)1770883646@qq.com;共同第一作者,高慧(1986-),女,山西晋城人,讲师,博士,主要从事中药、民族药资源开发与利用研究,(电子信箱)804204252@qq.com。
基金资助:
2024年广西研究生教育创新计划项目(YCSW2024431); 防城港市技术研究与开发财政补助资金项目(防科AD22013033); 广西壮族自治区科学技术厅广西创新驱动发展专项(桂科AA18118049); 2020年广西中药药效研究重点实验室运行补助项目(20-065-38); 广西中医药大学博士启动基金项目(2022BS016); 广西自然科学基金重点项目(2020GXNSFDA297029)

Study on the SSR genetic diversity of Camellia nitidissima var. phaeopubisperma

LI Shuang-shuang¹, GAO Hui¹, SU Meng-xue¹, CHEN Jin-mei¹, XIE Lei-hui^2,3, HUANG Yong-guang^2,3, LIN Xing³, DENG Jia-gang¹, XIE Yang-jiao¹

1. Guangxi University of Chinese Medicine, Nanning 530200, China;
2. Fangchenggang Association of Senior Scientists and Technologists, Fangchenggang 538021, Guangxi, China;
3. Fangchenggang Hospital of Traditional Chinese Medicine, Fangchenggang 538021, Guangxi, China

Received:2025-07-24 Published:2026-01-25 Online:2026-02-10

摘要/Abstract

摘要： 为探究防城金花茶(Camellia nitidissima var. phaeopubisperma)种质资源的遗传多样性,以同一产地但具有表型性状差异的111份防城金花茶为材料,采用SSR分子标记技术开展研究。结果表明,32对引物在111个样本中共检测到224个等位基因(Na),平均每个位点等位基因数目约为7。有效等位基因(Ne)总数为 95.22,平均有效等位基因数为2.976,观测杂合度(Ho)、期望杂合度(He)、香农指数(I)的平均值分别为0.475、0.562、1.194,多态性信息含量(PIC)平均值为0.528。分子方差分析(AMOVA)结果表明,总遗传变异中9%来源于群体间,91%来源于群体内(其中个体间占20%,个体内占71%)。基于非加权组平均法(UPGMA)、主坐标分析(PCoA)及STRUCTURE的聚类结果基本一致,均将111份样本划分为2个遗传类群,其表型特征与前期研究相符,进一步说明防城金花茶叶片表型性状的多样性与遗传背景密切相关。

关键词: 防城金花茶(Camellia nitidissima var. phaeopubisperma), 遗传多样性, SSR, 遗传结构

Abstract: To explore the genetic diversity of the Camellia nitidissima var. phaeopubisperma germplasm resources, a total of 111 samples of Camellia nitidissima var. phaeopubisperma from the same habitat with diverse phenotypic traits were analyzed using SSR molecular markers. The results showed that the 32 pairs of primers detected a total of 224 alleles (Na) across the 111 samples, with an average of approximately 7 alleles per locus. The total number of effective alleles (Ne) was 95.22, and the average effective allele number was 2.976. The average values of observed heterozygosity (Ho), expected heterozygosity (He), and the Shannon index (I) were 0.475, 0.562, and 1.194, respectively. The average polymorphism information content (PIC) was 0.528. Analysis of molecular variance (AMOVA) showed that 9% of the total genetic variation originated among populations, while 91% originated within populations (20% among individuals and 71% within individuals). The clustering results obtained from the unweighted pair group method with arithmetic mean (UPGMA), principal coordinate analysis (PCoA), and STRUCTURE analysis were largely consistent. All three methods classified the 111 samples into two genetic groups, and their phenotypic characteristics aligned with previous findings. This further confirmed that the diversity in leaf phenotypic traits of Fangcheng Camellia nitidissima was closely associated with its genetic background.

Key words: Camellia nitidissima var. phaeopubisperma, genetic diversity, SSR, genetic structure

中图分类号:

李双双, 高慧, 苏梦雪, 陈锦媚, 谢蕾卉, 黄永光, 林兴, 邓家刚, 谢阳姣. 防城金花茶SSR遗传多样性分析[J]. 湖北农业科学, 2026, 65(1): 97-104.

LI Shuang-shuang, GAO Hui, SU Meng-xue, CHEN Jin-mei, XIE Lei-hui, HUANG Yong-guang, LIN Xing, DENG Jia-gang, XIE Yang-jiao. Study on the SSR genetic diversity of Camellia nitidissima var. phaeopubisperma[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 97-104.

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防城金花茶SSR遗传多样性分析

Study on the SSR genetic diversity of Camellia nitidissima var. phaeopubisperma

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