富硒大豆根瘤菌的筛选鉴定及对大豆生长发育和硒积累的影响

doi:10.14088/j.cnki.issn0439-8114.2025.04.011

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (4): 58-63.doi: 10.14088/j.cnki.issn0439-8114.2025.04.011

富硒大豆根瘤菌的筛选鉴定及对大豆生长发育和硒积累的影响

胡叶青¹, 张杰², 何婷婷², 张悦², 李从虎², 刘芸双³, 胡婷²

1.安庆医药高等专科学校药学院,安徽安庆 246000;
2.安庆师范大学生命科学学院,安徽安庆 246000;
3.青岛市华测检测技术有限公司,山东青岛 266000

收稿日期:2024-08-22 出版日期:2025-04-25 发布日期:2025-05-12
通讯作者: 胡婷,安徽铜陵人,讲师,博士,主要从事功能性食品研究,（电子信箱）hutingby25@163.com。
作者简介:胡叶青（1989-）,女,安徽泾县人,讲师,硕士,主要从事中药物质基础及其机制研究,（电子信箱）huyeqing21@163.com。
基金资助:
安徽省教育厅项目（2023AH050470）; 皖西南地区生物多样性与生态保护省级重点实验室开放基金项目（Wsz202306）; 安徽省教育厅优秀青年教师培育一般项目（YQYB2023122）; 安徽省新型研发机构安庆市林业科技创新研究院开放基金项目（Ly202404）

Screening and identification of rhizobia from selenium-enriched soybean and their effects on growth and selenium accumulation of soybean

HU Ye-qing¹, ZHANG Jie², HE Ting-ting², ZHANG Yue², LI Cong-hu², LIU Yun-shuang³, HU Ting²

1. Department of Pharmacy, Anqing Medical College, Anqing 246000, Anhui, China;
2. College of Life Sciences, Anqing Normal University, Anqing 246000, Anhui, China;
3. Qingdao Huace Detection Technology Co., Ltd., Qingdao 266000, Shandong, China

Received:2024-08-22 Published:2025-04-25 Online:2025-05-12

摘要/Abstract

摘要： 从富硒大豆根瘤中筛选、鉴定出耐硒根瘤菌,分别采用硒酸钠、亚硒酸钠作为硒源,设定不同浓度梯度,研究根瘤菌的耐硒情况;并以中黄301为试验材料,采用田间试验,设置空白对照（CK）、根瘤菌处理（Rb）、亚硒酸钠处理（Se）、根瘤菌与亚硒酸钠结合处理（Rb+Se）,考察根瘤菌与硒元素结合处理对大豆[Glycine max (Linn.) Merr.]生长发育、硒积累的影响。结果表明,1.0 mg/L亚硒酸钠处理对根瘤菌生长具有一定的促进作用。与CK相比,Rb+Se处理大豆株高、主根长、单株干重分别提高78.67%、82.98%、69.57%,根、茎、叶、子粒、根瘤的硒含量分别提高3.53、2.44、1.37、2.63、1.82倍,土壤硒含量提高2.10倍;与Rb处理相比,Rb+Se处理大豆株高、主根长、单株干重分别提高21.45%、32.31%、14.71%,根、茎、叶、子粒、根瘤的硒含量分别提高113.04%、173.33%、65.21%、160.70%、49.50%,土壤硒含量提高125.65%;与Se处理相比,Rb+Se处理大豆株高、主根长、单株干重分别提高45.13%、56.31%、50.00%,根、茎、叶、子粒、根瘤的硒含量分别提高30.14%、50.01%、27.96%、22.59%、18.19%,土壤硒含量提高19.23%。由此可见,根瘤菌与硒元素的结合处理有利于大豆的生长发育,能够提高大豆各部位硒含量,同时对栽培土壤中硒积累也有一定提升。

关键词: 富硒大豆[Glycine max (Linn.) Merr.], 根瘤菌, 耐硒, 筛选, 鉴定, 大豆生长, 硒含量

Abstract: Selenium-tolerant rhizobia were screened and identified from selenium-enriched soybean[Glycine max (Linn.) Merr.] nodules. Sodium selenate and sodium selenite were used as selenium sources, and different concentration gradients were set to study the selenium tolerance of rhizobia. Zhonghuang 301 was used as the test material, and the field experiment was set up with blank control (CK), rhizobium treatment (Rb), sodium selenite treatment (Se), rhizobium and sodium selenite combination treatment (Rb+Se) to study the effects of rhizobia combined with selenium on the growth and selenium accumulation of soybean. The results showed that 1.0 mg/L sodium selenite treatment had a certain promoting effect on the growth of rhizobia. Compared with CK, the plant height, main root length and dry weight per plant of soybean treated with Rb+Se increased by 78.67%, 82.98% and 69.57%, respectively, the selenium content of roots, stems, leaves, grains and nodules increased by 3.53,2.44,1.37,2.63 and 1.82 times, respectively, and the selenium content of soil increased by 2.10 times. Compared with Rb treatment, the plant height, main root length and dry weight per plant of soybean treated with Rb+Se increased by 21.45%, 32.31% and 14.71%, respectively, the selenium content of roots, stems, leaves, grains and nodules increased by 113.04%, 173.33%, 65.21%, 160.70% and 49.50%, respectively, and the selenium content of soil increased by 125.65%. Compared with Se treatment, the plant height, main root length and dry weight per plant of soybean treated with Rb+Se increased by 45.13%, 56.31% and 50.00%, respectively, the selenium content of roots, stems, leaves, grains and nodules increased by 30.14%, 50.01%, 27.96%, 22.59% and 18.19%, respectively, and the selenium content of soil increased by 19.23%. It could be seen that the combination of rhizobium and selenium was beneficial to the growth of soybeans, which could enhance the content of selenium in various parts of soybeans, and also increase the accumulation of selenium in cultivated soil.

Key words: selenium-enriched soybean[Glycine max (Linn.) Merr.], rhizobium, selenium tolerance, screening, identification, growth of soybean, selenium content

中图分类号:

S65.1
S182

胡叶青, 张杰, 何婷婷, 张悦, 李从虎, 刘芸双, 胡婷. 富硒大豆根瘤菌的筛选鉴定及对大豆生长发育和硒积累的影响[J]. 湖北农业科学, 2025, 64(4): 58-63.

HU Ye-qing, ZHANG Jie, HE Ting-ting, ZHANG Yue, LI Cong-hu, LIU Yun-shuang, HU Ting. Screening and identification of rhizobia from selenium-enriched soybean and their effects on growth and selenium accumulation of soybean[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(4): 58-63.

参考文献

[1] 翟涛,吴玲.开放视角下中国大豆产业发展态势与振兴策略研究[J].大豆科学,2020,39(3):472-478.
[2] 沈琼,刘小和.我国油料、植物油的进口特征及品种间的替代性分析[J].中国农村经济,2006(5):25-31.
[3] 乔李锦,姚莉,徐冲,等.皖豫高效抗逆大豆根瘤菌Y2-4的选育[J].大豆科学,2024,43(1):45-53.
[4] MASSON-BOIVIN C, SACHS J.Symbiotic nitrogen fixation by rhizobia-the roots of a success story[J].Current opinion in plant biology, 2018, 44: 7-15.
[5] DAVID F, HERRIDGE, MARK B, et al.Global inputs of biological nitrogen fixation in agricultural systems[J].Plant and soil, 2008, 311(1): 1-18
[6] 刘颖,张佳蕾,李新国,等.豆科作物氮素高效利用机制研究进展[J].中国油料作物学报,2022,44(3):476-482.
[7] 杨乐. 刺槐-根瘤菌共生体系对镉污染土壤生物修复过程的微生物驱动机制[D].陕西杨凌:西北农林科技大学,2023.
[8] 陈长兰,郇丰宁,孟雪莲,等.硒对人体的作用机理及科学补硒方法[J].辽宁大学学报(自然科学版),2016,43(2):155-168,92.
[9] 付永霞,黄歆妤,胡锦蓉,等.加工方式对富硒大豆制品中硒含量的影响[J].食品科技,2018,43(8):164-169.
[10] 秦杰. 大豆根瘤菌的分离、筛选及结瘤相关性状的QTL定位[D].山西晋中:山西农业大学,2021.
[11] 王庆玲,霍雪雪,张豪,等.黄河口盐碱地大豆根瘤菌分离鉴定及田间应用[J].山东农业科学,2023,55(6):143-151.
[12] 黄志强,邱景璇,李杰,等.基于16S rRNA基因测序分析微生物群落多样性[J].微生物学报,2021,61(5):1044-1063.
[13] HU T, LI H F, LI J X, et al.Absorption and bio-transformation of selenium nanoparticles by wheat seedlings (Triticum aestivum L.)[J]. Frontiers in plant science,2018,9:597.
[14] 彭政,郭秀芝,徐扬,等.药用植物与根际微生物互作的研究进展与展望[J].中国中药杂志,2020,45(9):2023-2030.
[15] 张乐妍,陈春兰,王逗,等.化肥有机肥配施比例对油菜根际土壤利用光合同化碳微生物的影响[J].植物营养与肥料学报,2024,30(2):221-231.
[16] 程勤,胡承孝,明佳佳,等.硒对油菜根际土壤微生物的影响[J].农业资源与环境学报,2021,38(1):104-110.
[17] GARRIDO-OTER R, NAKANO R T, DOMBROWSKI N, et al.Modular traits of the rhizobiales root microbiota and their evolutionary relationship with symbiotic rhizobia[J]. Cell host & microbe, 2018, 24(1):155-167.
[18] CLÚA J, RODA C, ZANETTI M E, et al. Compatibility between legumes and rhizobia for the establishment of a successful nitrogen-fixing symbiosis[J]. Genes (Basel), 2018, 9(3):125.
[19] NYOKI D, NDAKIDEMI P A.Root length, nodulation and biological nitrogen fixation of rhizobium inoculated soybean (Glycine max [L.]Merr.) grown under maize(Zea mays L.)intercropping systems and P and K fertilization[J]. Advances in bioresearch, 2018, 9(1): 19-25.
[20] 李泽轩,冯亮,李开元,等.硒元素在茶园土壤和茶树内的富集规律[J].安全与环境工程,2023,30(6):217-223.
[21] 曹丹,马林龙,刘艳丽,等.茶树对硒吸收累积特性及其硒调控相关基因的表达分析[J].茶叶科学,2020,40(1):77-84.
[22] 孔娟娟,周守标,奚功芳,等.2种施硒方式对黄山贡菊生长、生理特性及硒积累的影响[J].水土保报,2014,28(4):310-316.
[23] 保虎德,马生军,陈文峰,等.外源硒结合根瘤菌处理对蒙古黄芪生长及其活性成分的影响[J].中国食品添加剂,2024,35(1):51-59.
[24] 范婷. 天然富硒大豆中含硒蛋白结构稳定性的研究[D].南昌:南昌大学,2016.
[25] GH/T1135—2017,富硒农产品[S].
[26] MISHRA P K, BISHT S C, MISHRA S, et al.Coinoculation of rhizobium leguminosarum-PR1 with a cold tolerant Pseudomonas sp. improves iron acquisition,nutrient uptake and growth of field pea (Pisum sativum L.)[J]. Journal of plant nutrition, 2012, 35(2): 243-256.
[27] 薛梅,陈悦,刘红芹,等.富硒肥的研究及其应用[J].中国土壤与肥料,2016(1):1-6.
[28] 冯德豪. 硒对大豆生长、籽粒及其制品硒含量和营养品质的影响研究[D].湖北荆州:长江大学,2022.
[29] GANG N, GUANGYU S, CHENGXIAO H, et al.Selenium improved the combined remediation efficiency of Pseudomonas aeruginosa and ryegrass on cadmium-nonylphenol co-contaminated soil[J]. Environmental pollution, 2021, 287:117552.
[30] CHENGJIAO D, YUXIA M, QIANG W, et al.Rhizobium inoculation enhances the resistance of alfalfa and microbial characteristics in copper-contaminated soil[J].Frontiers in microbiology, 2022, 12:781831.

富硒大豆根瘤菌的筛选鉴定及对大豆生长发育和硒积累的影响

Screening and identification of rhizobia from selenium-enriched soybean and their effects on growth and selenium accumulation of soybean

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