Study on fingerprint of Yinju mixture based on HPLC and chemometrics

doi:10.14088/j.cnki.issn0439-8114.2019.16.025

Abstract

Abstract: Using HLPC, Agilent Eclipse-C₁₈ column, detection wavelength 330 nm, mobile phase was methanol-0.2% phosphoric acid gradient elution, flow rate was 1.0 mL/min. 10 batches of Yinju mixture samples were studied by similarity evaluation of chromatographic fingerprints of traditional Chinese medicine. Results showed that thirteen common peaks were calibrated and three common peaks were qualitatively identified. The similarity of fingerprints was above 0.9, which met the requirements of fingerprint technology. The synergistic effect of multi-components affected the quality of preparations.

Key words: Yinju mixture, HPLC, chemometrics, fingerprint 。

CLC Number:

ZHANG Zhi, LYU Jian-wei, QIN Kai-yu, WU Min, ZHANG Bei, LI Jun-wei, WANG Ying, FENG Xiu-fang, LU Sen-hua. Study on fingerprint of Yinju mixture based on HPLC and chemometrics[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(16): 110-114.

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