Identification and biological characteristics of the pathogen from peanut web blotch in Liaoning province

doi:10.14088/j.cnki.issn0439-8114.2020.02.017

Abstract

Abstract: In order to confirm the pathogen of peanut web blotch in Liaoning province, the pathogenicity of pure cultures separated from the disease samples was determined by Kirsch's rule, and the pathogenic bacteria were identified according to their morphological characteristics and ITS sequence analysis. The biological characteristics of pathogenic bacteria were studied by colony growth method and concave glass slide germination method. The results showed that the pathogen of peanut netted spot disease in Liaoning province was Peyronellaea arachidicola. The optimum temperature of mycelium growth was 23 ℃. The best carbon source was maltose, the best nitrogen source was yeast paste, the optimum pH was 7, the light dark alternation was beneficial to the mycelium growth and the mycelial lethal temperature was 45 ℃. The optimum temperature for conidia germination was 25 ℃, the optimum pH was 7, and the illumination condition was beneficial to conidial germination.

Key words: peanut（Arachis hypogaea Linn.）, peanut web blotch, Peyronellaea arachidicola, biological characteristics

CLC Number:

S435.652

XIE Jin-hui, LIN Ying, ZANG Chao-qun, PEI Xue, LIANG Chun-hao. Identification and biological characteristics of the pathogen from peanut web blotch in Liaoning province[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(2): 82-86.

References

[1] 徐秀娟,崔凤高,石延茂,等.中国花生网斑病研究[J].植物保护学报,1995,22(1):70-74.
[2] 全鑫,宋玉立,何文兰,等.花生网斑病国内外研究进展[J].河南农业科学,2008,37(7):13-16.
[3] BUTT D J,ROYLE D J.Multiple regression analysis in the epidemiology of plant diseases[J].Epidemics of plant diseases,1990,13:78-114.
[4] MARASAS W F O,PAUER G D,BOEREMA G H. A serious leaf blotch disease of groundnuts (Arachis hypogaea L.) in southern Africa caused by Phoma arachidicola sp. nov[J].Phytophylactica,1974,6:195-201.
[5] AVESKAMP M M,GRUYTER DE J,WOUDENBERG J H C,et al. Highlights of the Didymellaceae:A polyphasic approach to characterise Phoma and related pleosporalean genera[J].Studies in mycology,2010,65(1):1-60.
[6] GLIENKE C,PEREIRA O L,STRINGARI D,et al.Endophytic and pathogenic Phyllosticta species,with reference to those associated with Citrus Black Spot[J].Persoonia,2011,26:47-56.
[7] 何晶晶,周如军,崔健潮,等.不同杀菌剂对花生网斑病菌室内毒力测定及田间防效研究[J].中国油料作物学报,2015,37(4):525-531.
[8] 傅俊范,王大洲,周如军,等.辽宁花生网斑病发生危害及流行动态研究[J].中国油料作物学报,2013,35(1):80-83.
[9] 傅俊范,崔建潮,周如军,等.辽宁花生主栽品种(系)对褐斑病和网斑病抗性鉴定[J].植物保护,2015,41(1):171-173.
[10] 袁虹霞,孙炳剑,李洪连,等.花生品种(系)对叶斑病的抗性鉴定[J].河南农业科学,2004,33(12):35-38.
[11] 徐秀娟,石延茂,徐明显,等.花生网斑病主要发生因子的关联性研究[J].山东农业大学学报,1992,23(4):430-434,438.
[12] 石延茂,徐秀娟,徐明显,等.花生网斑病经济阈值模型研究初报[J].莱阳农学院学报,1992,9(4):308-309.
[13] 李君彦,李有志,焦锋,等.陕西省花生网斑病病原菌的研究[J].花生科技,1991(1):1-6.
[14] 许欣然,张新友,汤丰收,等.花生网斑病病原菌分离及生物学特性研究[J].河南农业科学,2014,43(12):91-95.
[15] 董炜博,石延茂,赵志强,等.花生品种(系)叶部病害综合抗性鉴定[J].中国油料作物学报,2000,22(3):71-74.
[16] 方中达. 植病研究方法[M].北京:中国农业出版社,1998.
[17] 陆家云. 植物病原真菌学[M].北京:中国农业出版社,2001.
[18] LIU W,YE N X,CHEN Y S,et al.Identification and phylogenetic analysis of anthracnose pathogen Colletotrichum fructicola isolated from Camellia sinensis[J].Journal of tea science,2014,34(1):95-104.
[19] KITA Y,ITO M.Nuclear ribosomal ITS sequences and phylogeny in East Asian Aconitum subgenus Aconitum (Ranunculaceae),with special reference to extensive polymorphism in individual plants[J].Plant systematics and evolution,2000,225(1-4):1-13.
[20] 郑伟,王彬,彭丽娟,等.火龙果炭疽病病原鉴定与ITS序列分析[J].西南农业学报,2014,27(5):1970-1973.
[21] 陈永青,姜子德,戚佩坤.RAPD分析与ITS序列分析在拟茎点霉分类鉴定上的应用[J].菌物系统,2002,21(1):39-46.
[22] 陈亮,胡彦江,金静,等.温度、湿度和光照对花生网斑病菌分生孢子萌发的影响[J].青岛农业大学学报(自然科学版),2016,33(1):12-15.
[23] 朱茂山,白元俊,梁春浩,等.防治花生网斑病杀菌剂的筛选[J].农药,2017,56(10):763-766.
[24] 吕宾,陈殿绪,张霞,等.花生网斑病的田间药效试验[J].花生学报,2018,47(1):69-73.
[25] 李绍建,高蒙,王娜,等.花生网斑病不同病斑类型及其病原菌致病力差异[J].植物保护,2018,44(3):150-155.
[26] PETTIT R E,PHILLEY G L,SMITH D H,et al.Peanut web blotch:Ⅱ .symptoms and host rang of pathogen[J].Peanut science,1986,13(1):27-30.
[27] SMITH D H,MCGEE R E.Glycinemax:A potential host of the peanut web blotch fungus[J].Proceedings of the American peanut reaserch and education society,1981(13):100.
[28] 周如军,徐喆,王大洲,等.辽宁花生褐斑病发生及时间流行动态模型研究[J].中国油料作物学报,2014,36(4):533-537.
[29] 周如军,崔建潮,傅俊范,等.花生褐斑病菌和网斑病菌混合侵染对侵染概率和潜育期的影响[J].中国农业科学,2015,48(21):4264-4271.
[30] 崔建潮,周如军,傅俊范,等.花生褐斑病和网斑病田间混发流行过程及其产量损失研究[J].植物病理学报,2016,46(2):265-272.