HUBEI AGRICULTURAL SCIENCES

Advances in extraction,purification,chemical modification and antioxidant activity of polysaccharides

MENG Shu-yu, MERWERT·Adalhan, XIAYIDA·Nurmamate, ADELE·Suleiman, MULLIDER·Hederbai, CHEN Chun-li

2020, 59(2): 5-8. doi:10.14088/j.cnki.issn0439-8114.2020.02.001

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Polysaccharide has become a hot research topic in recent years because of its high activity, low toxicity, natural, wide source and so on. The extraction, purification, chemical structural modification and antioxidant activity of polysaccharide are reviewed.

Biological function and application of new plant growth regulator strigolactones

ZHENG Chen

2020, 59(2): 9-13. doi:10.14088/j.cnki.issn0439-8114.2020.02.002

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Strigolactones （SLs） is a general term of the alcohol compounds of striga. It is a kind of signal molecule which can regulate the endogenic development of plants in recent years. It is called a new plant hormone. Recently, much researches have been done on SLs, there are many reports on the study of striga compounds and their functions. The research and crop cultivation application of the biological functions of SLs were reviewed,and the future research of SLs was also forecasted.

Research advances on plant effects of engineered carbon nanomaterials

ZHANG Guan-lin, GAO Cun-bin, LIU Yu-zhen

2020, 59(2): 14-19. doi:10.14088/j.cnki.issn0439-8114.2020.02.003

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With the development of nanotechnology, engineered carbon nanomaterials are widely used in many fields. Meanwhile, ECNMs inevitably release into the environment, which will enhance the chances of contact with organisms. Biological toxicity and potential environmental risks can not be ignored. Plants are an important source of human food. At present, the researches have shown that ECNMs have toxic effects on plants and threaten food safety and human health. Based on the brief introduction of the environmental sources of ECNMs, the research progress of the effect and mechanism of ECNMs on the physiological growth of plants at home and abroad was summarized, the current research methods and challenges were discussed, so as to provide theoretical basis and practical reference for the biosafety assessment, plant protection and rational planning and using of raw materials of ECNMs.

Effects of nitrogen fertilizer and sowing methods on yield and nitrogen use efficiency of Xinmai No.9

XIE Xu-dong, ZHOU Guo-qin, CHEN Zhen-zhen, XU Hong, ZHANG Yu-bo, ZHANG Bo, LI Gang, LI Yu-feng

2020, 59(2): 20-23. doi:10.14088/j.cnki.issn0439-8114.2020.02.004

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From 2014 to 2015, the research on the yield and nitrogen use efficiency of Xinmai No.9 was carried out in Xinyang City,Henan Province. Three nitrogen fertilizer levels of 0, 150 and 225 kg/hm² were set, and two sowing methods of broadcast and drill seeding were set. The yield,dry matter accumulation and grain nitrogen content of Xinmai No.9 increased with the increase of nitrogen application. The highest yield were achieved when the nitrogen fertilizer level was 225 kg/hm² in the two sowing methods. With the increase of nitrogen supply, the nitrogen uptake of the plant above ground and the amount of grain nitrogen accumulation increased, but the nitrogen uptake efficiency, nitrogen utilization efficiency, nitrogen agricultural use efficiency, nitrogen partial factor productivity, and the apparent nitrogen recovery efficiency were all declined, and the nitrogen physiological efficiency increased. Under the current production level of wheat after rice in south Henan province, 225 kg/hm² of nitrogen application can obtain higher yield and economic benefits under the treatment of strip sowing.

Correlation analysis of essential amino acids,total amino acids and protein contents of different rice varieties

ZHANG Shun, LI Zhi-jian, ZHANG Yue-fei, YOU Yong, ZHANG Zhi-yuan, TANG Jian-zhou

2020, 59(2): 24-25. doi:10.14088/j.cnki.issn0439-8114.2020.02.005

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The essential amino acids, total amino acids and protein contents of 28 refined white rice samples were determined, and the correlation between the components of different refined white rice samples was analyzed. The results showed that the correlation coefficients of protein content and total amino acid content, protein content and essential amino acid content, total amino acid content and essential amino acid content of 28 samples were 0.940, 0.950 and 0.995, respectively. There was a very significant positive correlation between these indexes. The nutritional value of protein, the main nutrient component of white rice, mainly lies in the content of amino acids. The content of essential amino acids can be used as an important index to judge the nutrition of rice

Analysis on the characteristics of the yield structure of the approved japonica rice varieties in Henan province in 2009—2018

WANG Ling-yan, HUANG Jin-hua, WANG Shu-yu, ZHANG Su-ping, MA Hai-tao, LIU Zhen-yu, XIA Yan-li, DOU Shi-shu

2020, 59(2): 26-29. doi:10.14088/j.cnki.issn0439-8114.2020.02.006

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Using 36 japonica rice varieties approved in Henan province from 2009 to 2018, correlation analysis, partial correlation analysis and path analysis of their eight traits and yield were conducted to study the internal relationship and evolution of main agronomic traits affecting japonica rice. The results demonstrated that the average yield of japonica rice showed an upward trend, with an annual increase of 129.11 kg/hm², and the growth period showed a downward trend, which was 0.826 day per year, other characteristics didn’t change significantly. The eight traits have different effects on the yield of japonica rice. The number of grains per panicle is the main trait that affects the yield. The number of effective panicles, 1 000-grain weight and plant height are also important factors to yield. Therefore, under the premise of a reasonable and effective panicles, the main goal is to increase the number of grains and ensure the 1 000-grain weight.

Dynamic transformation of carbon in agricultural waste-compost and its fertilizer efficiency in citrus planting

LIAO Lei, XIONG Feng, ZHOU Yue-ming, LEI Yang-ming

2020, 59(2): 30-36. doi:10.14088/j.cnki.issn0439-8114.2020.02.007

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The dynamic composition and quantity of organic carbon in composting were analyzed by Van Soest analysis method, its influence mechanism on product quality during composting was also analyzed combined with procedure parameter. The results showed that, the duration of the high temperature period was 36 days, pH was 8.11, moisture content was 25.17%, OM was 52.34%, TN was 5.41%, effective number of viable bacteria was 9.6×10⁸ CFU/g, value of escherichia coli was 35 CFU/g, mortality of ascarid egg was 98%, germination index was 93.09%, which satisfied the requirements of NY 8884-2012 for biological organic fertilizer. The insoluble components of LIG, HEM and CEL in carbon composition were significantly correlated with total nutrients, OM, C/N（correlation coefficient was all greater than 0.875）. The degradation of insoluble macromolecular substances into small molecular substances which easily used by microorganisms is the key factors to improve the quality of fertilizer, whereas the loss of TOC will significantly reduce the quality of fertilizer. The application of the organic fertilizer in citrus plantations showed that the organic fertilizer could improve the quality and yield of citrus. The best quality and maximum yield of citrus were got with the application amount of 2.0 kg/m².

Preliminary report on application effect of CM biological nitrogen fixation fertilizers on rice

ZENG Yan-ling, LUO Zhi-yong, FENG Chun-shui, LI Mei-ling

2020, 59(2): 37-39. doi:10.14088/j.cnki.issn0439-8114.2020.02.008

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The effects of CM biological nitrogen fixation fertilizers on seedling quality, agronomic traits and yields were studied, and its application was explored in rice production. The results showed CM biological nitrogen fixation fertilizers improved the quality of seedlings and agronomic traits, which had obvious effects of reducing nitrogen, increasing yield and increasing income.

Influence analysis of meteorological factors on oleic acid of Camellia oleifera and model construction

PENG Jia-dong, LIAO Yu-fang, JIANG Yuan-hua, HUANG Chao

2020, 59(2): 40-43. doi:10.14088/j.cnki.issn0439-8114.2020.02.009

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Based on the collection and processing of the oleic acid data from multiple Camellia oleifera sample plots in different county of Hunan province during 2012—2017 and the meteorological indices for each stage of growth and treatment of Camellia oleifera which computed by the nearby meteorological observation stations’ data during the same period, the method of principal component analysis had been used to research the impacts of the meteorological factors in different stages on content of oleic acid for Camellia oleifera, and a forecasting model were constructed based on above meteorological factors. The results showed that the effects of meteorological factors for the whole year of fruit picking, oil transformation and accumulation peak period, fruit enlargement peak period on oleic acid content were more significant than other periods, and the precipitation indexes, especially the precipitation and the longest continuous precipitation days were the key factors which affecting oleic acid. The oleic acid forecasting model constructed base on those factors had a relative high accuracy and had certain applicability.

Analysis on climatic characteristics and influencing factors of hail days in Hunan province from 1979 to 2018

SANG You-wei, MENG Lei, HE Na, HUANG Wu-ying

2020, 59(2): 44-48. doi:10.14088/j.cnki.issn0439-8114.2020.02.010

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Hail days observation data from 95 weather stations in Hunan province and NECP 0.5°×0.5° reanalysis data from 1979 to 2018 was used to analyze the climatic characteristics and influencing factors of hail days in Hunan by linear trend estimation, M-K test, wavelet analysis, and similarity analysis. The results showed that the season of hail in Hunan province was obvious, the months from January to May are the main hail months, and the number of hail days in March was the most, which was a typical spring hail area; In general, hail in Hunan province was more in the northwest and less in the southeast, decreasing from northwest to southeast; In recent 40 years, the number of hail days in Hunan province had decreased significantly, and the decrease trend was the most significant in March, at the same time, the number of hail days presents periodic changes, especially in cycles 2~3 years and 4~6 years, 3 years cycle fluctuation had been the main trend since 2000; The decrease of hail days in Hunan province mainly came from the significant decrease of hail days in the areas with high incidence of hail, most stations in northwestern Hunan, northern Hunan, southern Hunan and central Hunan had a significant decrease of hail days; The spatial distribution and variation trend of hail in Hunan province were significantly correlated with the distribution and variation trend of atmospheric stability K index, in the regions with monthly average K index lower than 23, the high value area of hail corresponds to the relatively high value area of K index at the same latitude, and the regions with significantly decreased number of hail days correspond to the regions with significantly increased K index.

Distribution and risk evaluation of hail disaster of stagger-season strawberry production area in Sichuan province

ZOU Yu-jia, ZHANG Yu-fang, DAI Xin-lu, GUO Xiao-mei, HUANG Long-fei

2020, 59(2): 49-54. doi:10.14088/j.cnki.issn0439-8114.2020.02.011

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Based on the hail disaster data from 1980 to 2010, the temporal and spatial distribution of hail disaster in stagger-season strawberry production area of Sichuan province was analyzed. Combined with the geographic information data and hailstorm operation station data, the hailstorm disaster risk assessment model was constructed from three aspects of hazard risk, hazard bearing sensitivity and disaster prevention and reduction ability, and the hailstorm risk map was completed based on GIS. The results showed that the hail occurred every year in the stagger-season strawberry production area in Sichuan province, mostly from March to October, and the hail frequency showed a decreasing trend in 30 years. The number of hail days was the most in Ganzi prefecture and the least in Panzhihua-Xichang region. The risk of hail disaster gradually decreases from northwest to southeast, and the high risk areas are concentrated in the northwest and central parts of the northwest Sichuan plateau, including Shiqu county, Seda county and Litang county, while the low risk areas are mostly concentrated in Panzhihua-Xichang region.

Early warning of cultivated land quality in Henan province

SONG Yan-hua, WANG Ling-chao

2020, 59(2): 55-61. doi:10.14088/j.cnki.issn0439-8114.2020.02.012

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The cultivated land productivity early warning system is established to provide critical warning for regional cultivated land quality and provide early warning for the decline of cultivated land productivity. The negative number principle method is used to divide the warning threshold. Various models are established to calculate the change of cultivated land productivity caused by the different quantity of supplementary cultivated land and occupation cultivated land, the change of cultivated land productivity caused by the different quality of supplementary cultivated land and occupation cultivated land, the change of cultivated land productivity caused by the Land remediation. The results show that the change of cultivated land quantity has different influence on the quality and productivity of cultivated land. Affected by the different quality of supplementary cultivated land and occupation cultivated land, the relationship between cultivated land quantity and quality is non-positive but the relationship between cultivated land quantity and productivity is positively. Affected by different causes of cultivated land quality change, the change of cultivated land quality and productivity is not proportional to each other. Cultivated land productivity can better reflect the health status of regional cultivated land protection. The construction of cultivated land quality can improve the quality and productivity of cultivated land when the quantity cannot be balanced. The analysis of the early warning results and causes of cultivated land can provide more targeted measures for regional cultivated land protection. It is concluded that cultivated land productivity can be used as an early warning index of cultivated land quality. Early warning of cultivated land quality can avoid the serious deterioration of cultivated land quality. The early warning results can provide real-time services for the continuous utilization of land resources. The results have certain foresight to cultivated land grain production. The results are helpful to ensure the stability and safety of food production under sustainable development.

Purification evaluation and management of fish pond water quality by ecological floating bed：Taking the fish pond of Huayu Garden Nursery stock ecological garden as an example

LAI Xin, FU Jin, ZHANG Xi-zhi

2020, 59(2): 62-66. doi:10.14088/j.cnki.issn0439-8114.2020.02.013

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Taking the fish pond of Huayu Garden Ecological Nursery as the research object, total nitrogen （TN）, total phosphorus （TP）, ammonia nitrogen and chemical demand （COD） in water before and after planting ecological floating bed as evaluation indexes, the water quality was tested. Respectively from two dimensions of time and space,the absorption effect of ecological floating bed on nitrogen and phosphorus in water and the influence trend of of COD in water were analyzed and compared. The results showed that the ecological floating bed for the whole water of nitrogen and phosphorus and COD has certain absorption effect, including removal rate up to 15.2% of total nitrogen, total phosphorus removal rate up to 56.3%. The management concept of ecological floating bed for fish pond water purification was put forward.

Research on the management of action mechanism between population spatial factors and green development space

JIANG Yu-xiao, ZHU Ming-cang, GU Ji-rong, ZHAO Yuan-di

2020, 59(2): 67-73. doi:10.14088/j.cnki.issn0439-8114.2020.02.014

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Under the concept of inclusive economic growth with the theme of green development, research the feedback mechanism between the population spatial factors and green development space. By using geographic information method and spatial analysis technology, and based on the monitoring results of geographical conditions, the spatial elements of green development were extracted to form the spatial pattern of green development. At the same time, the spatial carriers of social and economic factors were extracted, the statistical indicators of social economy were quantified, and the traditional resource factors and innovation-driven factors in social economy were measured. Using the multiple logistic regression model to construct the feedback equation between population spatial factors and green development space, to establish the feedback mechanism between them. It can be found from the study of Chengdu that the growth of innovative population improves the efficiency and potential of land use, leading to the decrease of land demand, and the population density in the same space unit can be increased by up to 49%. The increase of innovative population has a positive incentive effect on green development, while the increase of traditional population has a negative incentive effect on green development.

Gini coefficient of ecological security of water resources in 7 districts of Shangluo city

LIU Yan, LI Xuan

2020, 59(2): 74-78. doi:10.14088/j.cnki.issn0439-8114.2020.02.015

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Based on the basic principles of the Lorenz curve and the Gini coefficient calculation method, the degree of matching between water resources and cultivated land resources, GDP, population and grain yield makes in Shangluo city were studied. The Gini coefficient corresponding to the water resources and cultivated land area, GDP, population and grain yield in Shangluo city was 0.201 4, 0.174 6, 0.251 0 and 0.236 0, respectively. The analysis showed that the match of the water resources and cultivated land resources, GDP, population and grain yield were good, concluding the overall level of ecological Shangluo city was good. And the corresponding countermeasures and suggestions for the future development of regional economy in Shangluo city was put forward.

Experimental comparison of germplasm resources different varieties of Astragalus sinicus

FAN Mei-rong, ZHANG Chun-xia, LIAO Yu-lin, XIE Bai-cheng, LU Yan-hong, NIE Jun

2020, 59(2): 79-81. doi:10.14088/j.cnki.issn0439-8114.2020.02.016

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The growth period, characteristics, yield of fresh plant, nutrient content of four varieties of Astragalus sinicus on pot-experiment were compared and analyzed. The results showed that Xiangzi No 3 and Xinyang Astragalus sinicus were more suitable to plant in double-cropping rice area than other varieties. The plant characters of Xiangzi No 3 such as leaf color, plant height, branch number, true leaf number, root length, number of rhizobia before winter were all better than the other varieties. There was no significant difference in stem diameter, number of branches and weight per plant among Yujiang large leaves, Yuanjiang specie and Xiangzi No 3 at the full-bloom stage, and these indexes were significantly higher compared with Xinyang Astragalus sinicust. The fresh grass yields of the four varieties were manifested included： Yujiang Large Leaves >Yuanjiang Species>Xiangzi No 3>Xinyang Astragalus sinicust at the full-bloom stage. Xiangzi No 3 had the highest total nitrogen and total potassium content among the four varieties. There was no significant difference in total phosphorus content among the four varieties.

Identification and biological characteristics of the pathogen from peanut web blotch in Liaoning province

XIE Jin-hui, LIN Ying, ZANG Chao-qun, PEI Xue, LIANG Chun-hao

2020, 59(2): 82-86. doi:10.14088/j.cnki.issn0439-8114.2020.02.017

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In order to confirm the pathogen of peanut web blotch in Liaoning province, the pathogenicity of pure cultures separated from the disease samples was determined by Kirsch's rule, and the pathogenic bacteria were identified according to their morphological characteristics and ITS sequence analysis. The biological characteristics of pathogenic bacteria were studied by colony growth method and concave glass slide germination method. The results showed that the pathogen of peanut netted spot disease in Liaoning province was Peyronellaea arachidicola. The optimum temperature of mycelium growth was 23 ℃. The best carbon source was maltose, the best nitrogen source was yeast paste, the optimum pH was 7, the light dark alternation was beneficial to the mycelium growth and the mycelial lethal temperature was 45 ℃. The optimum temperature for conidia germination was 25 ℃, the optimum pH was 7, and the illumination condition was beneficial to conidial germination.

Evaluation of the control effect of biocontrol bacteria Bacillus amyloliquefaciens D2WM on Dickeya chrysanthemi and its fungicides screening

CHEN Jia-min, LIU Hui-ying, ZHANG Xiang-hui, DENG Ting-ting, WEI Mi

2020, 59(2): 87-92. doi:10.14088/j.cnki.issn0439-8114.2020.02.018

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Single factor test was used to optimize the extraction efficiency of Bacillus amyloliquefaciens D2WM antimicrobial substance, and the storage characteristics of crude extraction of antimicrobial substance were studied. The stability of crude extracts and the fungicides screening on Dickeya chrysanthemi were studied. The results of biocontrol agents and commercial agents were compared. The results indicated that the optimum extraction solvent was n-butyl alcohol, the volume ratio of organic solvent to fermentation broth was 1∶7, and the extracting time was 0.5 h. In addition, the antimicrobial substances showed excellent stability towards temperature and ultraviolet. The antimicrobial activity was not influenced when exposed at 100 ℃, or after treatment of ultraviolet irradiation for 12 hours, the size of the antimicrobial circle was still 23 mm. When the antimicrobial substances were stored for 180 days, the size of the antimicrobial circle was still 16.33 mm. The crude extracts of biocontrol bacteria Bacillus amyloliquefaciens D2WM had good bacteriostatic effects on plant pathogens such as Pectobacterium atrosepticum, Pectobacterium carotovorum, Dickeya chrysanthemi, Pseudomonas syringae and Xanthomonas arboricola. Only New-tian Xiucai No. 6, Zhongshengmycin, benziothiazolinone and chloroisobromine cyanuric acid had good inhibitory effects on Dickeya chrysanthemi among the 19 tested medicines. It was found that the bacteriostatic effect of crude extract of biocontrol bacteria Bacillus amyloliquefaciens D2WM was higher than benziothiazolinone and chloroisobromine cyanuric acid.

Effects of tolfenpyrad on toxicity and behavior of Solenopsis invicta worker ants

HUANG Shi-you, CHEN Hui-ya, MA Qian-li, WANG Yong-qing, ZHANG Zhi-xiang

2020, 59(2): 93-97. doi:10.14088/j.cnki.issn0439-8114.2020.02.019

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The effects of tolfenpyrad on the behavior of worker ants of Solenopsis invicta Buren were studied by using water tube to feed poison method. After 10 h treatment with 5, 10 and 20 mg/L tolfenpyrad, respectively, the mortality rate of the small worker ants was 58.3%, 97.5% and 83.3%, much higher than that of the control group with only 10% of the mortality rate. The medium mortality rate of red fire ants was 51.7%, 86.7% and 60.0%, respectively, which was significantly higher than that of the control group with 3.3%. After 10 h treatment of 0, 5, 10 and 20 mg/L, the walking rates of small red imported fire ants was 93.3%, 31.7%, 0, 3.3%, the grasping rate was 93.3%, 21.7%, 0, 3.3%, and the climbing rate was 93.3%, 21.7%, 0, 3.3%, respectively. The walking rate of red fire ants was 96.7%, 63.3%, 8.3% and 31.7%, the grasping rate was 96.7%, 46.7%, 5.0% and 20.0%, and the climbing rate was 96.7%, 55.0%, 5.0% and 26.7%, respectively.

Study on the identification of Magnoliaceae plants based on the alcohol-soluble protein

YE Shan-shan, WANG Nan, GAO Yan-fang, TANG Mi, LIU Meng-ling, XIAO Qiang

2020, 59(2): 98-101. doi:10.14088/j.cnki.issn0439-8114.2020.02.020

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The Magnoliaceae plants are widely distributed and varied in genus and species. There are many controversies in the identification of genus and species because many transitional types were found among different genus and species. At present, botanical shape characteristics are usually used as identification methods for the classification and identification of magnoliaceae plants. In order to further identify the magnolia plants, the acid polyacrylamide gel electrophoresis was used to analyze the gliadin of magnolia plants. The results showed that 61 bands were separated from 10 species of magnolia,and most of the bands were between 6 and 7, with a maximum of 9, and the lowest 6. Using Quantityone software to analyze electrophoresis bands, it is found that there are differences between different bands, and species identification can be carried out through spectral bands.

Research on the detaining dust plant community structure in urban road

CHEN Ying-jia, LIU Zhong-bing

2020, 59(2): 102-105. doi:10.14088/j.cnki.issn0439-8114.2020.02.021

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The study on the community structure of urban road plants was reviewed and analyzed, and the connotation and construction of the structure of the plant community in the urban road were put forward. The stability and variability of plant community structure, the combination of detaining dust function and multi-function, the construction and development of detaining dust plant community structure and green space system were discussed.

Effects of different light quality on the coloration of the leaves of the Crassula capitella‘Campfire’ of crassulaceae

LIU Mei-zhou, LI Yi-zhu, ZHANG Rui, GE Jia-rong, REN Yi, ZHANG Xue, LIU Xiao-jia, QI Yi-jie

2020, 59(2): 106-109. doi:10.14088/j.cnki.issn0439-8114.2020.02.022

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Taking the leaf cutting seedlings of annual Crassula capitella ‘Campfire’ as materials, the effects of light quality on color difference, chlorophyll and anthocyanin content of annual Crassula capitella ‘Campfire’ leaves were studied after three months of treatments with white light,red light,blue light and ultraviolet, then the effects of light quality on the coloration of the Crassula capitella ‘Campfire’ leaves were analyzed. The results showed that different light quality was important in the coloration process of Crassula capitella ‘Campfire’ leaves. Among them,ultraviolet had the greatest effect on the color difference and red saturation of Crassula capitella ‘Campfire’ leaves, and effectively increased the content of carotenoids and anthocyanins in leaves, and had the best effect on promoting the color of Crassula capitella ‘Campfire’ leaves.

Changes of endogenous growth regulators during roots of tissue culture seedlings of Lonicera macranthoides Hand.-Mazz

CAO Zheng-yan, TANG Ning, CHEN Ze-xiong, LOU Juan

2020, 59(2): 110-113. doi:10.14088/j.cnki.issn0439-8114.2020.02.023

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The tissue culture seedlings of Lonicera macranthoides Hand.-Mazz were used as experimental materials,the changes of the content of indole-3-acetic acid （IAA）, abscisic acid （ABA）, gibberellic acid （GA₃）, and zeatin nucleoside （ZR） in the root-inducing process （0,6,12,18,24,30 d） were determined by the method of enzyme-linked immunosorbent assay（ELISA）. The results showed that the content of ABA was increased initially and then decreased. The concentrations of IAA, GA₃ and ZR displayed the opposite trends, which were decreased firstly and subsequently increased. The contents of IAA and GA₃ and the ratio of IAA to ABA were significant or extremely significant positively correlated with rooting rates, while ABA contents were negatively correlated with rooting rates, and there was no significant correlation between ZR content and rooting rate.

Analysis of the correlation between the soaking time and the emergence rate of the seed of Ricinus communis L.

JIN Fang-lun, LUO Chao-bin, LUO Hui-xian, WU Kang-yun, WANG Xian-yu, JIN Ling

2020, 59(2): 114-119. doi:10.14088/j.cnki.issn0439-8114.2020.02.024

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The correlation between the soaking time and the emergence rate of the seed of Ricinus communis L. was investigated and studied. Results showed that there was a significant negative correlation between the different soaking time of Ricinus communis L. seeds and the total number and rate of emergence of seeds, but no significant correlation with other indexes. The quadratic curve regression equation and linear regression equation were used to analyze the different soaking time, the total number of seedlings, the total rate of emergence and the rate of no nest of Ricinus communis L. seeds. The results showed that the regression equation of the different soaking time and the total number of seedlings, the total rate of emergence and the rate of no nest of Ricinus communis L. seeds were all in accordance with the quadratic curve regression equation.

Geographical indication characteristics and unique production technology of rice in Huanghekou

LIU Yan-gang

2020, 59(2): 120-122. doi:10.14088/j.cnki.issn0439-8114.2020.02.025

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It was recognized as a national agricultural product geographical indication product in 2011. The geographical indication characteristics of rice in Huanghekou were expounded, including specific production area, unique production environment, human history and unique product quality, the unique production mode of rice in Huanghekou was summarized from the aspects of production area selection, variety selection, production management process, harvest and processing, aiming to maintain the brand quality of rice in Huanghekou to the greatest extent and further improve the quality of rice in Huanghekou brand awareness and market competitiveness of Huanghekou rice in the national and even international market.

New cultivars of Lagerstroemia indica Lagerstroemia indica ‘Danzhao Zihuan’ and Lagerstroemia indica ‘Honghuan Danzi’

WANG Ye-she, CHEN Li-jun, YANG Xian-jun, DUAN Lin-dong

2020, 59(2): 123-124. doi:10.14088/j.cnki.issn0439-8114.2020.02.026

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The new varieties of Lagerstroemia indica Lagerstroemia indica ‘Danzhao Zihuan’ and Lagerstroemia indica ‘Honghuan Danzi’ were selected through collecting widely seeds of various sources and varieties of crape myrtle, developing of mixed seed grow seedlings and using natural hybridization seedling-selection method. After cultivation experiments, the two new varieties are fine garden flower tree species because they have the characteristics of stable genetic character, wide adaptability, strong stress resistance and high ornamental value, and has been applied in city landscaping.

Study on the main flavor amino acids of the hemp chickens in the free-range breeding mode in northern mountains of Guangdong：Take Qingyuan pai chicken as an example

CHENG Tian-de, ZHONG Nan

2020, 59(2): 125-127. doi:10.14088/j.cnki.issn0439-8114.2020.02.027

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The amino acids of hydrolysate of raw chicken and cooked chicken of Qingyuan pai chicken in the free-range breeding mode have been determined and analyzed by automatic analysis of amino acid. The results showed that the species of free amino acid were almost the same between the hydrolysate of raw chicken and the cooked chicken, but the content of amino acid in hydrolysate of raw chicken was significantly higher than cooked chicken, the content of those amino acids which taste bitter in hydrolysate of raw chicken（Arg, Lys, His, Met and Val） was higher and the TAV was bigger, the main umami amino acid （Glu） in proportion is obviously improve in cooked chicken than in the raw chicken, the main sweet amino acids in raw chicken hydrolysate and cooked chicken （chicken soup） were Ala, but the content and TAV were lower.

Comparative study of three kinds of rhubarb processed products on the draining effect of Dachengqi decoction in mice

QIU Mei-yan, WEI Jiang-cun, ZHANG Ru, XIE Zhen, JIANG Xian-zai, HAN Qian, ZHAO Feng-xian

2020, 59(2): 128-132. doi:10.14088/j.cnki.issn0439-8114.2020.02.028

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To study the difference of the purging effect of rhubarb, vinegar rhubarb and rhubarb charcoal on Dachengqi decoction, 96 experimental mice were randomly divided into positive control group, blank control group, DCQ 6,10 g/kg dose group, vinegar DCQ 6,10 g/kg dose group and carbon DCQ 6,10 g/kg dose group. The activities of rhubarb, rhubarb and Dachengqi decoction of rhubarb and rhubarb on normal diarrhea, propelling rate of carbon and Na⁺-K⁺-ATPasc activity in mice were compared. Results showed that the effects of rhubarb, rhubarb and rhubarb charcoal on the diarrhea of Dachengqi decoction were different, but the variation of Dachengqi decoction was related to rhubarb, rhubarb and rhubarb carbon. Compared with the blank control group and the positive control group, the DCQ group, the vinegar DCQ group and the carbon DCQ group had different effects on normal diarrhea, the propulsive effect of carbon end and the inhibition of the activity of Na⁺-K⁺-ATPase in normal mice, and the difference of the effect between the DCQ group and the DCQ group was the most significant. The effects of rhubarb, rhubarb and rhubarb carbon on the diarrhea of Dachengqi may be different, which may be related to the changes in the content of the main chemical composition of the radix dahuris, rhubarb and rhubarb charcoal.

Study on storage application of carton packing sheet smoke without lining bag

SU Zan, SUN Jian-sheng, LI Ji-gang, TANG Yu-xin, HU Ya-jie

2020, 59(2): 133-136. doi:10.14088/j.cnki.issn0439-8114.2020.02.029

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In order to verify the applicability of the storage technology of carton packing sheet tobacco non-lined plastic bag in large-scale storage of tobacco raw material warehouse, the appearance quality, chemical composition and sensory quality were measured by selecting six grades of tobacco leaves in four production areas and adopting the comparison storage of non-lined bags and lining bags. The results showed that after 18 months of storage, compared with the tobacco with inner lining bag, the smoke without inner lining bag had a lighter color change, better chemical composition and better sensory quality. It provided the basis for improving the curing quality and optimizing the store storage technology.

Effect of different storage temperature on quality and weight loss rate of sweet potato

YAN Hai-feng, LI Wei-liu, XIONG Jun, WEI Ming-zhen, TANG Xiu-hua

2020, 59(2): 137-140. doi:10.14088/j.cnki.issn0439-8114.2020.02.030

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To examine the effect of storage temperature on quality and loss rate of sweet potato for its suitable storage, Guijingshu 8 was stored at room temperature and at temperature of （9±0.5） ℃ with humidity of （85±5）%,respectively, and weight loss rate, dry matter content, total soluble sugar, starch content, protein and vitamin C content were determined after 0,15,30,45,60 and 75 days storage, respectively. The results showed that compared with the room temperature storage, the weight loss rate reduced by 7.1 percent, the dry matter content reduced by 3.06 percent, and the total soluble sugar increased with 51.06% in 9 ℃ after 75 days storage. The content of starch and protein was not significantly different between the room temperature and 9 ℃ after 75 days storage. The content of vitamin C reduced by 56.21% in room temperature, but only reduced by 41.62% in 9 ℃ after 75 days storage, the content variation exhibited significantly difference between the two storage conditions at this time. But the variation of the vitamin C content between room temperature and 9 ℃ storage was not significantly different after 75 days storage. In conclusion, 9 ℃ was beneficial for Guijingshu 8 storage.

Extraction technology of anti-inflammatory constituent asperuloside from Psychotria serpens L.

HONG Ying-wen, LIANG Zi-ning, LIAO Gui-xiu, WEI Li-cui, LONG Qi-yun

2020, 59(2): 141-144. doi:10.14088/j.cnki.issn0439-8114.2020.02.031

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HPLC was used to establish methods of content determi-nation of asperuloside in Psychotria serpens L.. Asperuloside as index components,on the basis of methanol concentration,liquid ratio and extraction time,we carried out experiments of single factor and orthogonal design to further optimize the extraction process. Optimum extraction of Glycyrrhizic flavonoids：70% methanol,solid-liquid ratio 1∶60,extraction time 30 min.

Process optimization of chlorogenic acid in honeysuckle of Shangluo by ultrasound-assisted alcohol extraction

HE Jun

2020, 59(2): 145-148. doi:10.14088/j.cnki.issn0439-8114.2020.02.032

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Aimed at probing a suitable extraction process for local production with the the honeysuckle of Shangluo city. Based on the alcohol extraction method, ultrasound-assisted extraction was carried out with the orthogonal test results on the basis of single factor test. The optimal extraction process of chlorogenic acid in honeysuckle of Shangluo were as followed ethanol mass fraction was 50%, ultrasonic time was 40 min, ultrasonic temperature was 65 ℃, solid-liquid ratio was 1∶40, the extraction rate of chlorogenic acid could be 4.305%.

Analysis of volatile components extracted from Magnoliae flos and mixed product by HS-SPME-GC-MS

HAN Man, JIANG Han-mei, LU Jin-qing, DAI Yu-xin

2020, 59(2): 149-152. doi:10.14088/j.cnki.issn0439-8114.2020.02.033

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The volatile components extracted from Magnoliae flos（dried flower buds of Magnolia biondii Pamp.） and mixed product （dried flower buds of Magnolia liliflora Desr.） were analyzed by HS-SPME-GC-MS. Results showed that 30 and 41 compounds were separated and identified from Magnoliae flos and mixed product respectively,there were 12 common components, the highest content of the volatile components in Magnoliae flos is g-Terpinene（26.68%）, and the highest content of the volatile components in mixed product is galcanfor （35.67%）.

Design on cut twist micro-plough

SUN Peng, MAO Ting-ting, QIU Wan-li, WANG Yuan

2020, 59(2): 153-156. doi:10.14088/j.cnki.issn0439-8114.2020.02.034

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In order to solve the problem that the micro tillage machine was easily entangled by weeds, low efficiency and high labor intensity during the cultivation of Yunnan mountain, a new type of micro tillage machine was designed, which was simple in structure, small in size and easy to operate. Through field experiments, it was concluded that this machine can not only guarantee the stability of soil tillage, but also improve the soil breaking rate and achieve the desired effect.

Rice planting area information extraction based on multi-temporal remote sensing data

PENG Le-wen, ZHANG Ya

2020, 59(2): 157-160. doi:10.14088/j.cnki.issn0439-8114.2020.02.035

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In remote sensing images, the moisture content and soil moisture of plants were sensitive to the short-wave infrared band, while the red band and near-infrared band reflected the coverage rate and growth rate of plants strongly. According to the characteristics of rice growing in moist soil, based on a decision tree rice extraction model with time difference, this paper carried out relevant research on rice growing area extraction in sheyang county, yancheng city, jiangsu province by calculating NDVI and LSWI of normalized vegetation index and soil moisture index at different growth periods. The comparison of the extracted rice area and local statistical data showed that the model can effectively distinguish the water area, corn and vegetable fields that were easily confused with rice, and the precision of area extraction reached 76.26%.

Estimation model of precipitable water vapor based on deep LSTM neural network

LUO Yu, LUO Lin-yan, FAN Jia-zhi, DUAN Si-ru, GAO Wen-juan

2020, 59(2): 161-165. doi:10.14088/j.cnki.issn0439-8114.2020.02.036

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Based on deep long short term memory（LSTM） neural network, the estimation models （LSTM₅ and LSTM₁） of precipitable water vapor （PWV） in Huaihua distinct are proposed by using multi（air temperature, air pressure, dew point temperature, relative humidity, water vapor pressure and hourly precipitation） and single factor（water vapor pressure） separately, besides the estimation precision of the models are analyzed. As the result shown, the LSTM models have high estimation precision, whose coefficients of determination are both greater than 0.94, mean values of root square error, mean absolute error and mean absolute percentage error are below 1.158 1 mm, 0.709 9 mm and 4.54% respectively. The precision of LSTM models improves more than 70% compared to linear estimation model or quadratic polynomial estimation based on water vapor pressure, and the precision of LSTM₁ model slightly better than that of LSTM₅. The distribution of estimation errors relates to PWV value, which is first increased and then decreased along with the increasing of PWV. Furthermore, there is negative correlation between the estimation precision of the models and altitude of the stations.

The Cep164 gene indirectly regulate CP110 expression in the centriole

WANG Chun-hua

2020, 59(2): 166-168. doi:10.14088/j.cnki.issn0439-8114.2020.02.037

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Cep164 is one of the important genes involved in ciliogenesis. In order to study the relationship of the Cep164 and CP110, explored the role of CP110 in the regulation of ciliogenesis by Cep164. Immunofluorescence staining and Western Blotting were used to determine the expression of CP110 gene in the Cep164 gene knockout cell and embryo of mice. The interaction between the Cep164 and CP110 protein was confirmed by COIP. The results showed the Cep164 gene mutation increased CP110 gene expression. Cep164 regulated the expression of CP110 in cells indirectly. CP110 degradation failure could be the one of reason for the Cep164 gene mutation cause cilia dysfunction.

Preliminary study on optimization of industrial layout in Gansu province based on water ecological constraints

LI Fei, ZHANG Xiao-ping

2020, 59(2): 169-175. doi:10.14088/j.cnki.issn0439-8114.2020.02.038

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In order to coordinate the relationship between water ecological environment and the development of regional industry, based on the two aspects of water resources and water environment, a zoning index system for the water ecological constraints in Gansu province was constructed, and a division and zoning method of the water ecological constraints was proposed. On the basis of water resource constraint zone and water environment constraint zone, through the two-dimensional quadrant analysis of water resources and water environment constraints in each region, the water ecological constraints of the whole province were divided into four types of water ecological constraint zones, including high water resources constraint and high water environmental constraint, high water resources constraint and low water environmental constraint, low water resources constraint and high water environmental constraint, low water resources constraint and low water environmental constraint, among them, the industrial layout of Jiayuguan city and Gannan prefecture has relatively good aquatic ecological adaptability. In this regard, the key transformation of industrial upgrading in Gansu province, the industrial environment access conditions, and the optimization of industrial layout are proposed. The research results can provide theoretical and methodological references for other areas to coordinate the relationship between industrial development and the relationship between water resources and water environment.

Research on the evaluation of the protection of farmers’ rights by economic law on the PSR-AHP model

YANG Bei

2020, 59(2): 176-179. doi:10.14088/j.cnki.issn0439-8114.2020.02.039

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The protection of farmers’ rights and interests is facing a major practical problem of the status of farmers in vulnerable groups. In order to prevent farmers from getting deeper and deeper in the vortex of vulnerable groups, it is necessary to specify the principles of economic law from the perspectives of balance and coordination, fairness and efficiency, extreme promotion and extreme restrictions, and to protect farmers' rights from this perspective. In order to evaluate the effect of economic law on the protection of farmers' rights and interests, the PSR-AHP model was introduced to construct an evaluation index system, and an empirical analysis was conducted using Shaanxi province as an example. The results show that economic law has a certain effect on the protection of farmers’ rights and interests, but there is a certain lag in the legislative and law enforcement levels. Future economic law reforms need to be improved from these two aspects in order to truly exert the effectiveness of protecting peasants' rights and interests.

Analysis on the responses of college students’ innovation and entrepreneurship education to e-commerce poverty alleviation：Evidence from the Pearl River Delta and west Guangdong

WANG Ming-dui

2020, 59(2): 180-185. doi:10.14088/j.cnki.issn0439-8114.2020.02.040

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In order to explore the internal mechanism of the response of college students’ innovation and entrepreneurship education in the Pearl River Delta and western Guangdong to e-commerce poverty alleviation, using the PSR model, and selecting 21 variables such as the rate of change in farmers’ income, with evaluation value conversion, comprehensive evaluation value measurement, coordination degree calculation and other methods, the pressure-state-response level of the study area was analyzed. The results show that the pressure of the study area is concentrating towards the Pearl River Delta, but Guangzhou, Foshan, and Shenzhen are still in a dominant position in the state and response. The negative correlation between the comprehensive evaluation score and the evaluation value of the degree of coordination in the study area shows that the crisis hidden in the region is widespread.

A study on the supply of public services for farmers and herdsmen relocated from inhospitable areas——Based on the analysis of Wuqia county,Xinjiang Uygur Autonomous region

ZHOU Chong-chong, YANG Jun-xiao

2020, 59(2): 186-189. doi:10.14088/j.cnki.issn0439-8114.2020.02.041

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Change of poverty alleviation relocation involved many aspects, such as public service, industry employment, Wuqia county in Xinjiang Uygur Autonomous region topaz port change of poverty alleviation and moving point of practical investigation and research, for example, summed up the public service supply results and analyzed its existing problems, and put forward to realize the diversification of the rural public service supply, improve the farmers demand expression ability, strengthen community cadre public service ability and so on suggestion.

Research on the demand of knowledge payment products based on the group perspective of network interest

HE Li-shi, WANG Hong-peng, ZHU Hui

2020, 59(2): 190-193. doi:10.14088/j.cnki.issn0439-8114.2020.02.042

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Based on net interest margin group perspective to study the knowledge characteristics of paid products and user demand, found that users use knowledge demand pay product was the use of fragmented time to accumulate interdisciplinary knowledge and skills, in order to solved the knowledge anxiety, screening high quality content and services, access to experted in the field of cognitive and insight, seek sentimental, relieve the huge pressure from society. By referring to the typical case of knowledge payment product demand to meet the design, put forward the knowledge payment product development Suggestions.

Research on the path of farmers’ participation in rural tourism projects under the background of precision poverty alleviation：Based on a sustainable livelihoods framework

PAN Xin, ZHANG Jiao-jiao, LIU Yi-ran, LI Xue-ting

2020, 59(2): 194-200. doi:10.14088/j.cnki.issn0439-8114.2020.02.043

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Based on the sustainable livelihood framework, statistical analysis of the livelihood capital scores of 236 rural households in two state-level poverty-stricken counties, Zigui county, Hubei and Pingshan county, Hebei, a was taken, and the logistic model was used to explore the relationship between livelihood capital owned by farmers and rural tourism participation. The results show that social capital has the most significant impact on farmer participation in tourism projects, followed by financial capital, physical capital, and natural capital, and human capital is the least significant. The natural capital of Jiuwan Stream Scenic Area is significantly higher than that of Foguang Mountain Scenic Area, the difference between other capitals is not obvious, and it has similar effects on farmers' participation in rural tourism projects. Due to the lack of livelihood capital, the participation of rural poverty-stricken households is significantly lower than that of ordinary farmers. Efforts to increase the existing social and financial capital stocks of farmers are of great significance to motivate farmers, especially poor households, to participate in rural tourism projects, and at the same time help improve the livelihood results of poor households, thereby achieving precision poverty alleviation.

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