Development of new Hongshancaitai line with clubroot resistance

doi:10.14088/j.cnki.issn0439-8114.2024.12.022

Abstract

Abstract: Using clubroot resistance （CR） Brassica rapa ssp. chinensis var. purpurea germplasm GD1 as the resistant donor, and using DGZ1902, an early mature strain of Hongshancaitai, as the background material, backcross breeding was conducted. The penta-primer amplification refractory mutation system （PARMS） marker for CR gene CRzi8 carried by CR donor was developed and furtherly applied in the marker assistance selection （MAS）, single plant selection and identification of root disease inoculation, finally the new CR Hongshancaitai line was created. The results showed that the PAMRS marker for CRzi8 could accurately detect the CR individuals from the backcross breeding program. Field resistance test and agronomic traits evaluation indicated that the developed CR Hongshancaitai line CR-DGZ1902 was resistant to Plasmodiophora brassicae pathotype 4, and had both agronomic characteristics of Hongshancaitai and early maturity characteristics of DGZ1902.

Key words: Hongshancaitai, molecular marker, clubroot disease, resistance gene, new line

CLC Number:

S634.6

REN Zhi-yong, DONG Bin-feng, HE Qing-song, NIE Qi-jun. Development of new Hongshancaitai line with clubroot resistance[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(12): 121-124.

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