Genetic diversity analysis of mitochondrial DNA D-loop region in Yunxi Matou goat

doi:10.14088/j.cnki.issn0439-8114.2025.07.034

Abstract

Abstract: The genetic diversity of Yunxi Matou goat（Capra hircus） was evaluated based on genetic variation analysis of mitochondrial DNA (mtDNA) D-loop region sequences.The complete mtDNA D-loop sequences of 184 Yunxi Matou goats were obtained by PCR amplification and sequencing, followed by systematic analysis of nucleotide polymorphism and haplotype diversity, neutrality tests (Tajima’s D and Fu’s Fs), mismatch distribution analysis, and cluster analysis with mtDNA D-loop sequences from different goat breeds.The results showed that the proportions of A, G, C, and T bases in mtDNA D-loop of Yunxi Matou goat were 30.77%, 14.26%, 26.26%, and 28.71% respectively, with A+T content (59.48%) significantly higher than G+C content (40.52%).There were 52 haplotypes and 131 variable sites in mtDNA D-loop of Yunxi Matou goat, including 51 singleton variable sites and 80 parsimony-informative sites. Fu’s Fs test showed Fs value of -2.48, indicating significant deviation from the neutral model (P< 0.05).The phylogenetic tree of Yunxi Matou goat and other five goat breeds (Chuandong White goat, Shannan White goat, Huanghuai goat, Beichuan White goat, and Boer goat) showed that 184 individuals were divided into three major branches, including two native branches and one Boer goat hybrid branch.The Yunxi Matou goat population showed high polymorphism and rich genetic diversity with great breeding potential, but exhibited genetic introgression from surrounding local goat breeds and Boer goat.

Key words: Yunxi Matou goat（Capra hircus）, mitochondrial DNA, D-loop region, genetic diversity

CLC Number:

S827

CHENG Lei, LIU Chen-hui, CHEN Yang, XIANG Min, YU Jie, ZHONG Zhu-xia, XIA Yong-chun, YANG Wu-jun, HU Xiu-zhong. Genetic diversity analysis of mitochondrial DNA D-loop region in Yunxi Matou goat[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(7): 198-202.

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