Biological characteristics of Phytophthora nicotianae resistant mutants to mandipropamid

doi:10.14088/j.cnki.issn0439-8114.2026.02.018

Abstract

Abstract: In order to clarify the biological characteristics, genetic stability of drug resistance, and pathogenicity of mandipropamid-resistant strains of Phytophthora nicotianae, the P. nicotianae strain YCHJ was used as the tested strain. Mandipropamid-resistant strains of P. nicotianae were obtained by UV-irradiating. The biological characteristics of mutant strains were compared with those of their parent strain such as colony morphology, hyphal growth rate, zoosporangia morphology and production, and germination ability of zoospores. The genetic stability and pathogenicity of the mutant strains were assessed by the mycelial growth rate method and greenhouse pot trials, respectively. The results showed that the resistance levels of the mutant strains YCHJ2-12, YCHJ5-7 and YCHJ5-16 induced by mycelial UV-irradiating were 62.99, 47.06 and 10.11, respectively. The resistance levels of the mutant strains YCHJZ2-2 and YCHJZ4-13 induced by UV-irradiating of zoospores were 54.79 and 17.75, respectively. The results of biological characteristics showed that there was no significant difference in hyphal morphology and zoosporangia morphology between the mutant strains and the parent strain, but there were differences in the mycelial growth rate, the size of zoosporangia and the height of papillae. The average mycelial growth rate of YCHJ2-12 and YCHJZ2-2 was significantly higher than that of the parental strain YCHJ. The length, width and papillae height of the zoosporangia produced by YCHJZ4-13 were significantly higher than those of YCHJ. In addition, the zoosporangia production of YCHJZ4-13, YCHJ5-16 and YCHJZ2-2 was significantly higher than that of YCHJ, but the germination rate of zoospores and pathogenicity of the mutant strains were lower than those of YCHJ. The resistance of YCHJZ4-13 and YCHJ5-16 to mandipropamid couldn’t be stably inherited, and the resistance of other strains decreased with the increase of cultivation generations. Based on these, it was speculated that the resistance risk of P. nicotianae to mandipropamid was low to moderate.

Key words: tobacco black shank, mandipropamid, resistant mutant, biological characteristics

CLC Number:

S572

PENG Yu, CHENG Jia-wen, LI Cai-bin, MA Qian, MA Yu-xi, HE Yi, WU Xue-qiao, ZHOU Wen-liang, DENG Jian-qiang, QI Yong-xia. Biological characteristics of Phytophthora nicotianae resistant mutants to mandipropamid[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 120-125.

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