Study on Micro-tubulin Gene Ghtub12 to Cotton in Gossypium hirsutum

doi:10.14088/j.cnki.issn0439-8114.2018.18.027

Abstract

Abstract: Cotton is the world's important fiber crop, cotton tubulin gene in cotton fiber development process specific advantages of expression, affecting cotton fiber quality. Tubulin gene Ghtub12 as a member of the tubulin gene family, the mechanism of cotton fiber development is not clear. In this experiment, R15 hypocotyls were used as acceptor, and 0.5 mg/L Basta was used as a screening marker. Agrobacterium tumefaciens-mediated transformation method was used to study the genetic transformation. 100 calli were induced by resistance callus, and 143 callus were obtained after 1 month. The recovery rate was 71.50%. After 3 times, the callus was obtained and the callus was obtained. The transformation rate was 81.00%. Among them, 45 callus induced embryogenic callus, the differentiation rate was 44.12%. Embryogenic calli were divided into 60 seedlings. The positive rate was 93.3%. The transgenic tubulin gene Ghtub12 was obtained, which provided experimental material for further study on the possible mechanism and effect of Ghtub12 gene in cotton.

Key words: Gossypium hirsutum Linn. R15, Gossypium tubulin gene Ghtub12, agrobacterium-mediated method, hypocotyls

CLC Number:

S562

SHEN Zhe-nan, SHEN Qiu-ping, PENG Qiu, LYU Zun-fu, LI Fei-fei. Study on Micro-tubulin Gene Ghtub12 to Cotton in Gossypium hirsutum[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(18): 106-110.

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