HUBEI AGRICULTURAL SCIENCES

Research advances in plant cyclic nucleotide-gated channels

WANG Xin-yue, LIU Pei-yuan, YU Bing-qing, ZHANG Yan-feng, DING Bai-xiang, YAN Han-chi

2021, 60(8): 5-7. doi:10.14088/j.cnki.issn0439-8114.2021.08.001

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Cyclic nucleotides （CNMPs）may represent signal moleculesof many signaling pathways in plants. The activity of cyclic nucleotide-gated channels（CNGCs）in plant can be regulated by cyclic nucleotides, participating in plant growth and development as well as responding tovarious stresses. In this review,the molecular structural characteristics, regulation, ion selectivity and function of CNGCs in plantsare reviewed in detail. The new advances in CNGCs in plant growth and stress response is summarized. The research direction of CNGCs is also prospected in order to provide theoretical basis for the research of CNGCs in plant.

Research progress of mitophagy in plants

JIANG Ya-ting

2021, 60(8): 12-16. doi:10.14088/j.cnki.issn0439-8114.2021.08.002

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Mitophagy is widely found in animals, plants and yeast, playing an important role in maintaining the balance of mitochondria in cells. Compared with the research progress of mitophagy in yeast and mammals, the research of mitophagy in plants is still at an early stage. And the mechanism of mitophagy in plants and the proteins involved in autophagy are still unclear. This paper reviews the research progress of mitophagy and the regulatory mechanism of mitophagy in plants, as well as related receptors, proteases and biological functions, so as to provide reference for the research on mitophagy in plants in the future.

Study on the mechanized recovery of mulch film

LI Wei, LIAO Pei-wang, YU Jia-chuan, ZHUO Dong-ling, ZHANG Ai-min

2021, 60(8): 17-22. doi:10.14088/j.cnki.issn0439-8114.2021.08.003

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China has been faced with increasingly severe mulch film pollution. Currently, the primary treatment mode of film residue pollution is mechanized film recovery. It is introduced that the machines and tools for recovering residual film, the importance of plastic film quality, the storage and transportation of mechanical residual film, and the problems of resource utilization. It is expounded that the mechanized recovery of plastic film is system engineering involved the whole industrial chain of plastic film production, use, recovery, storage, transportation and resource utilization.

Results of cultivation density and row spacing of giant rice

LUO Yan-ping, WEI Qiao, HOU Ling, LUO Dong-yu, LI Hong-ju, PAN Dian-jin, ZHOU Jia-hua, ZHANG Xin-lin, LUO Wen-qing, WU Ji-ping

2021, 60(8): 23-27. doi:10.14088/j.cnki.issn0439-8114.2021.08.004

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Through the quadratic general rotary unitized design to study the high-yield cultivation mode of giant rice Fengchao No.6,two controllable factors of cultivation density and row spacing was selected, and the temperature and light difference in the field was recorded. Analyzing the mathematical model between actual yield and two factors, it is concluded that the two-factor level configuration with a yield of more than 9 000 kg/hm² is cultivation density 86 064~92 880 plants/hm², row spacing 43.94~50.78 cm.

Breeding and cultivation techniques of new maize variety Guidan662

HUANG Ai-hua, HUANG Kai-jian, WEI Xin-xing, MO Run-xiu, TANG Zhao-lei, ZHAI Rui-ning, ZOU Cheng-lin, WEI Hui, TAN Hua

2021, 60(8): 28-31. doi:10.14088/j.cnki.issn0439-8114.2021.08.005

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Guidan 662 is a new maize variety which is bred by crossing Gui A10341 as female parent and GML906 as male parent in Maize Research Institute of Guangxi Academy of Agricultural Sciences. In 2017, the average yield of maize in Guangxi was 8 253.6 kg/hm², which was 8.4% higher than that of the control Guidan 162. In 2018, the average yield of maize in Guangxi was 7 348.1 kg/hm², which was 6.5% higher than that of the control Guidan 162. From 2017 to 2018, the average yields of new maize varieties were 9 616.5~9 243.0 kg/hm² respectively, which were 18.2% and 16.6% higher than that of the local variety LVN10, it participated in maize new-variety selection trial and maize contrast test in Natha Province of Laos. In 2019, it was approved by both the Laos Crop Variety Registration （accession number：00080S） and the Crop Variety Certification Committee of Guangxi Zhuang Autonomous Region （accession number：Guishenyu 2019087）. Guidan 662 has strong disease resistance, wide adaptability and good commercial property. It is suitable for planting in all parts of Guangxi and all provinces of Laos.

Effect of inoculation of arbuscular mycorrhizal fungi on growth and quality of pea in subsidence area of coal mining in northern Shaanxi

WU Qun-ying, MA Shao-peng, BI Yin-li, GAO Ya-kun, ZHOU Hui-li, ZHANG Long-jie

2021, 60(8): 32-35. doi:10.14088/j.cnki.issn0439-8114.2021.08.006

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In this study, arbuscular mycorrhizal fungi （AMF） were inoculated in the loess land of Zhangjiamao mining area in north Shaanxi province, and the common local legume plant pea （Pisum sativum L.） was planted to monitor its growth and quality and yield, and soil physical and chemical properties. The results showed that the root colonization rate of pea increased by 16.11 percentage points, and a good symbiotic relationship between mycorrhizal and pea could be formed. Compared with the control treatment, the plant height and SPAD of pea increased by 52.34% and 29.35% （P<0.05）, the biomass and yield of straw increased by 22.69% and 20.61%, the total nitrogen and potassium content of mycorrhizal plant increased by 12.72% and 22.19% （P<0.05）, and the total nitrogen and potassium absorption of pea plant per hectare increased by 38.74% and 51.89%, respectively. The soluble protein and sugar of pea per hectare increased by 20.09% and 47.99%, respectively. Mycorrhizal inoculation increased soil conductivity, available phosphorus, available potassium, organic matter, total potassium, total phosphorus and total nitrogen, and decreased pH, but the difference was not significant. Inoculated mycorrhiza can promote the productivity of plants in the Loess Plateau, and microbial reclamation can provide a good potential for the improvement of productivity in the Loess Plateau.

Effects of chemical capping agent on direct cotton after rape

ZHANG Li-juan, CHEN Jun-ying, QIN Yu-kun, WANG Yu-ping, CHENG Hui-huang, XIA Shao-nan

2021, 60(8): 36-40. doi:10.14088/j.cnki.issn0439-8114.2021.08.007

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The effects of chemical capping agent on the growth and development of early-maturing cotton after rape direct seeding was studied under the machine-picking mode, the results provided a basis for the maturation, demonstration and promotion of this technique in the cotton planting area of Poyang lake in the Yangtze river basin. In 2017—2018, the Zhongmiansuo 50 and Zhongmiansuo 425 were used as test materials to perform live broadcast after rape harvesting in machine mode, with 8 treatments （spraying time were T1 and T2; spraying doses were D1, D2 and D3, respectively, and no topping and artificial topping as controls, respectively, CK1 and CK2） to study its agronomic characteristics and yield of cotton effects on properties and quality.After spraying the chemical capping agent, the growth of cotton plants in the vertical and horizontal directions was suppressed at the same time, forming a compact plant type. Compared with artificial topping, using chemical agents to cap, as long as you master the spray application time and dosage will not reduce cotton yield and have no obvious impact on cotton fiber quality.According to the results of 2 years, under the climate and production conditions in the cotton planting area of Poyang lake, chemical agents were used to cap the top of direct seeding cotton after early maturingrape, and the concentration of the capping agent was 1 125~1 500 mL/hm², the application time should be about 5 days later than artificial topping.

Effects of different exogenous selenium on selenium accumulation and distribution and yield components of peanut

YU Qiong, ZHANG Xiang, SI Xian-zong, SUO Yan-yan, LI Liang, YU Hui

2021, 60(8): 41-45. doi:10.14088/j.cnki.issn0439-8114.2021.08.008

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Using Yuhua 37 as the experimental material, a pot experiment was conducted to explore the effects of three selenium sources （Na₂SeO₃, SeMet and SeCys） on agronomic characters, selenium content, selenium accumulation, distribution and yield composition of peanut. The results showed that at the same level of selenium application, the application of organic selenium sources （SeMet and SeCys） could promote plant growth better than inorganic selenium sources, and increase the height of main stem, the length of first branch and the number of branches. The content of selenium in all parts of peanut plants was the highest in the roots, followed by kernels. The selenium content and selenium accumulation of peanut kernel from inorganic selenium sources on were higher than those from organic selenium sources. The order of selenium accumulation in different parts of the plant was as follows： Kernel（5.40~16.75 μg/plant）>leaf（3.33~4.86 μg/plant）>stem（2.11~3.47 μg/plant）>shell（2.05~3.23 μg/plant）>root （0.85~1.75 μg/plant）. The distribution ratio of selenium accumulation showed that the proportion of kernel was the largest and that of root was the lowest. The yield, 100-pod weight and full pods weight per plant of organic selenium source were higher than those of inorganic selenium source （Na₂SeO₃）, and the yield of SeMet and SeCys treatments increased significantly by 22.5% and 18.8% compared with Na₂SeO₃ treatment. Soil application of three kinds of exogenous selenium had different effects on agronomic characters, selenium content and cumulative distribution proportion and yield components of peanut.

Determination the manure decomposition and nutrient release the Sophora alopecuroides

ZHANG Chao-yang, WANG Lin-na, WANG Meng, XI Lin-qiao

2021, 60(8): 46-49. doi:10.14088/j.cnki.issn0439-8114.2021.08.009

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The decomposition law of green manure and the nutrient release law of C, N, P and K in soil of Sophora alopecuroides during vegetative period and early flowering period were studied by nylon bag method. The results showed that after 118 days of decomposition, the mineralization rate of N and P in green manure increased gradually, and the mineralization rate of C in green manure was 66.59%. K mineralized rapidly from 0 to 7 days, with a mineralization rate of 55.46%, and mineralized 79.06% at 118 days. C/N showed a downward trend from 0 to 14 days, and became gradually stabilized from 14 to 118 days, and the nutrient mineralization rate was K>C>P>N. On 118 days, the dry matter（DM） decomposition of green manure reached 65.2%, and the mineralization rates of C, N, P and K reached 62.75%, 44.93%, 52.54% and 79.06%, respectively. Among them, 0~14 d was the rapid decomposition stage, 14~28 d was the slow decomposition stage, and 28~118 d was the slow decomposition stage. The dry matter degradation and nutrient release of Sophora alopecuroides green manure were basically synchronized and positively correlated.

Assessment of ecological vulnerability in Jinsha river basin

SHANG Jia-ning, SHAO Huai-yong, LI Feng, OUYANG Xin

2021, 60(8): 50-54. doi:10.14088/j.cnki.issn0439-8114.2021.08.010

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Taking the Jinsha river basin with a complex natural environment and important ecological functions as a research area, an evaluation index system was constructed from two aspects of natural environment and human activities, and a spatial principal component analysis model was used to quantitatively evaluate the ecological vulnerability of the Jinsha river basin from 2005 to 2015. The evaluation results were graded and counted, and then the distribution characteristics and change trends of its ecological vulnerability were obtained. The results showed that the ecological fragility in the Jinsha river basin was significantly different, the upstream ecological environment was better, the middle reaches was second, the downstream was more fragile, and the areas river flow was less vulnerable than the two banks of the valley; The overall ecological environment in the river basin was at a medium level, and the proportions of vulnerability levels from high to low were mild fragile, moderate fragile, potential fragile, severe fragile, and extreme fragile; During the three study periods from 2005 to 2015, the comprehensive index of ecological vulnerability （EVSI） of the Jinsha river basin were 2.53, 2.55 and 2.38, respectively, which firstly increased slightly and then decreased, coupled with the continuous reduction of extremely vulnerable areas, and the overall ecological environment had improved.

Ecosystem health evaluation of wetland in the middle reaches of Heihe river based on PSR model

DU Hong-xia, SUN He-zhou

2021, 60(8): 55-62. doi:10.14088/j.cnki.issn0439-8114.2021.08.011

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In order to quantitatively evaluate the health status of the wetland ecosystem in the middle reaches of Heihe river on a spatial-temporal scale, based on the PSR （pressure-state-response） model, 11 indicators were selected from 3 target layers of pressure, state and response to build an index system. The AHP method was used to determine the weight of each index, and combined with the comprehensive index method, the ecological health of the wetland in the middle reaches of Heihe river in the past 18 years was evaluated. The results showed that the health status of the middle reaches of Heihe river was poor, good, good and average in 2000, 2005, 2011 and 2018, respectively. Among them, the wetland ecosystem in Minghua township of Yugur autonomous county of Sunan, which was at a poor level in these four years. The state layer with the most influence （0.650）, while the response layer was the smallest （0.120）. Normalized difference vegetation index （NDVI）, human disturbance index and landscape diversity index （SHDI） were the main factors affecting the health of wetland ecosystem in this region.

Effects of different fertilization management on soil nutrient dynamic of citrus orchard in the Three Gorges Reservoir Area

WANG Xiao-rong, FU Tian, TANG Wan-peng, CUI Hong-xia, HU Wen-jie

2021, 60(8): 63-69. doi:10.14088/j.cnki.issn0439-8114.2021.08.012

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The citrus orchard in the Three Gorges Reservoir Area was choose as a research object to detect the effects of different fertilization management approaches such as integrated fertilization with water and fertilizer, traditional fertilization, mixed fertilizer fertilization on soil nutrient dynamics. The results showed that it was acidic soil in citrus orchard that suitable for citrus growth. In 0~10 cm soil layer, the organic matter, total nitrogen, total phosphorus, available nitrogen, available phosphorus and available potassium content under integrated fertilization with water and fertilizer management were significantly higher than those under traditional fertilization and mixed fertilization management. In 10~30 cm layer, the soil nutrients were no significant different among the three fertilization measures in citrus orchard. Compared to soil nutrients in April, the soil organic matter, total nitrogen, total phosphorus, total potassium content under different fertilization managements were lower in July, while the available nitrogen, available phosphorus and available potassium content were higher. The results indicated that the integrated fertilization with water and fertilizer can maintain the soil nutrient in a high level, while the soil under traditional fertilization was still lack of nutrient content which meand the excessive fertilization can not improve the soil nutrient consistently. In the future, applying organic fertilizer with low intensity and high frequency will be an effective way to keep enough available nutrients in citrus orchard. At the same time, soil and water conservation measures, such as grass cover and hedgerows, should be strengthened to reduce the loss of nutrients on the surface and underground leaching, so as to reduce the risk of non-point source pollution.

Research on provincial ecological space delineation based on “ecological protection red line,environmental quality baseline,resource utilization upper limit and ecological environmental access list”：A case study of Zhejiang province

TANG Bo, WANG Li-mei, MAO Hui-ping, XU Ming-zhu, WANG Zhe-ming

2021, 60(8): 70-73. doi:10.14088/j.cnki.issn0439-8114.2021.08.013

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Zhejiang province, the south wing of the Yangtze river delta region, was selected as the research area to carry out the research on ecological space demarcation. By adopting the method of “ecological protection red line, environmental quality baseline, resource utilization upper limit and ecological environmental access list”,combining with the reality of Zhejiang province,the ecological space of Zhejiang province was identified, and a total of 54 535.79 km² of land ecological space was delimit, accounting for 52.28% of the land area of Zhejiang province.

Study on the fine distribution characteristics of falling water affected by complex topography in Hubei province

SUN Peng-jie, WANG Bi-qiang, CHEN Zheng-hong, ZHANG Rong, JIAN Shi-lue

2021, 60(8): 74-80. doi:10.14088/j.cnki.issn0439-8114.2021.08.014

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Using the rainfall data of 2 005 regional automatic meteorological observation stations and 82 National weather stations in Hubei province for the past three years, combined with the longitude, latitude, height, slope, and slope of the digital elevation data（DEM）, precipitation and terrain factor models were established in eight regions. Compared with the spatial interpolation distribution of actual precipitation, the trend was relatively consistent. The accuracy of the model simulated precipitation was 89.5%, and the model can better reflect the actual precipitation situation. From the results of simulation, the annual precipitation in most parts of Hubei province was between 600 and 2 100 mm, and its distribution trend increases from north to south. The three regions of southwestern Hubei, southeastern Hubei, and northeastern Hubei were the regions with the most precipitation. Considering the factors such as mountain area, latitude and longitude, the simulated annual precipitation in the mountain area was obviously larger than that of the precipitation distribution based on the national weather station data only, in which the precipitation in the southeast of Hubei was 200~300 mm larger, more than 300~400 mm in southwest Hubei and about 300 mm in northeast Hubei. In particular, the precipitation distribution model of Hubei province can reflect the characteristics of precipitation in high altitude areas significantly higher than the surrounding low-altitude areas.

Research on the impact of local climate on the differences of low temperature anomalies in Xuzhou city

SUN Lei, LYU Xiang, HU Shen-shen, WANG Ke-wei

2021, 60(8): 81-86. doi:10.14088/j.cnki.issn0439-8114.2021.08.015

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The data of daily minimum temperature and corresponding climatic background at 6 artificial stations in Xuzhou city were collected. Taking Xuzhou station as the standard station, the error distribution between stations was analyzed, and the data were classified into two types： Two-terminal type, eastern type, western type and whole type. By using the methods of orthogonal function and statistical analysis, the correlation characteristics of the main meteorological factors which cause the great difference of temperature between stations, including wind direction, wind speed, humidity, cold air, etc., were analyzed and the correction methods were refined. The results showed that Feng county was the most prone to appear obvious errors; When the average wind speed was less than 0.5 m/s, the influence of wind direction was not considered. When the average wind speed was more than 0.5 m/s, Xuzhou city was not conducive to cooling when it was affected by southerly wind. Pei county was not conducive to cooling when it was affected by east to southeast wind and Suining county was affected by south to southwest wind. The characteristics of wind direction in Feng county, Pizhou city and Xinyi city were not obvious; The occurrence frequency of large-value errors was highest in autumn and winter in the west, and highest in spring and least in summer in the east; When there was no cold advection, the influence of local wind direction and wind speed environment was dominant, the two-terminal type should be considered, and when there was cold advection, the western type and eastern typeshould be paid more attention. Through subjective revision, the error of site could be reduced and the level of fine forecasting could be further improved.

Identification and biological control of tobacco target spot disease （Rhizoctonia solani Kühn） in Sichuan tobacco planting areas

XU Chuan-tao, ZHANG Chong, ZHANG Ming-jin, XIE Qiang, PENG Yong, ZHANG Yong-hui, XIA Jian-hua, XIA Chun, XIA Bo, GU Yong, WU Yuan-hua

2021, 60(8): 87-90. doi:10.14088/j.cnki.issn0439-8114.2021.08.016

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Isolation, identification, pathogenicity and mycelial fusion group determination of pathogenic bacteria collected from tobacco area of Luzhou, Sichuan province were carried out, and several biological pesticides were selected for field control experiments. The results showed that the pathogen was identified as Thanatephorus cucumeris （Frank） Donk, and its anamorph was Rhizoctonia solani Kühn. Observation for the mycelium fusion showed that all the isolates belonged to the anastomosis group 3 （AG-3）. The control effect of Jinggang mycin 10% AS, Bacillus marinus WP and 10.5 billion CFU/g of mixture for Paenibacillus polymyxa and Bacillus subtilis WP were 77.08%, 69.40% and 63.75%,respectively.

Study on the control effect of Grapholitha molesta by using sex pheromone directed method in Jurong city peach orchard,Jiangsu province

YANG He-tong, XUE Zheng, CHENG Jia-qian, ZOU Kai-yu

2021, 60(8): 91-93. doi:10.14088/j.cnki.issn0439-8114.2021.08.017

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In order to research the control effect and reasonable using density by sex pheromoneisotropic vector of Grapholitha molesta, using the technology the control test was carried outin peach orchard. The results showed that better control effect were 450, 600 and 990 pieces/hm² treatment respectively, height density treatment was better. Broken end rate and borer fruit rate of Grapholitha molesta were lower than the control, control effect of broken end rate of 450, 600 and 990 pieces/hm² treatment were 94.79%,96.58% and 96.90%, respectively, and control effect borer fruit rate were 92.55%,96.09% and 97.51%, respectively. Environmental concentration of sex pheromone was 600 pieces/hm²to be reasonable from considering the comprehensive prevention effect and cost.

Stability analysis of antimicrobial active protein from Ochrobactrum sp. SY286

AN Fu-tao, XIE Jin-hui, LIN Ying, YU Shu-yi, ZANG Chao-qun, LIANG Chun-hao

2021, 60(8): 94-97. doi:10.14088/j.cnki.issn0439-8114.2021.08.018

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Antibacterial active proteins were extracted from the fermentation filtrate of Ochrobactrum sp. SY286 by saturated ammonium sulfate precipitation method. The stability of the active substance was determined by the influence of temperature, pH, ultraviolet light and protease. The results showed that the activity protein salts out from saturation of 60% ammonium sulfate precipitation exhibited the strongest inhibitory activity against Plasmopara viticola, and the inhibition rate was up to 81.98%. The temperature, pH and ultraviolet light had certain influence on active protein. The active protein showed stability under condition of lower than 50 ℃ and pH 4~10. Proteinase had great impact on inhibiting activity substances. Proteinase K, trypsase, trypsase and nematolyt could suppress its activity.

Effect of pinoxaden·mesosulfuron-methyl on weed control,nutrient and yield in wheat field

ZHU Wen-da, YAN Dong-dong, LI Lin, ZHOU Yong-an, CAO Ao-cheng

2021, 60(8): 98-101. doi:10.14088/j.cnki.issn0439-8114.2021.08.019

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The effect of 6% pinoxaden·mesosulfuron-methyl OD on weed control in wheat field was studied. The wheat yield, the content of N-P-K and water were also analyzed after weed control by pinoxaden·mesosulfuron-methyl. The results indicated that the efficacy of weed control were remarkable by 6% pinoxaden·mesosulfuron-methyl WDG in wheat field. The control efficacy on Malachium aquaticum, Polypogon fugax and Beckmannia syzigachne was ranged from 90.8% to 97.5%. The accumulation of weed water and fertilizer was significantly decreased after weed control. The wheat yield was ranged 3 482.52 to 3 603.77 kg/hm² after treated with pinoxaden·mesosulfuron-methyl, which increased by 36.17%~40.91% compared to untreated control. Moreover, the yield income increased by 2 127.5~2 406.4 yuan/hm².

Effects of low-voltage direct current electric field on the growth of Astragalus adsurgens Pall. and soil enzyme activity in root system

CHEN Xin-yan, LING Meng, DENG Liu-yang, DU Meng-lin, YANG Yin-yin, WANG Chen, CHEN Ke

2021, 60(8): 102-106. doi:10.14088/j.cnki.issn0439-8114.2021.08.020

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The seedlings of Astragalus adsurgens Pall. were treated with low-voltage direct current electric（DC）field of different intensity.The changes of plant height, plant stem, root length, biomass, chlorophyll content, fluorescence kinetics parameters, antioxidant enzyme activities and soil enzyme activities of root system were measured 30 days after adding electricity. The results showed that in the growth process of Astragalus adsurgens Pall. seedlings, the Fv/Fm of seedlings in the three regions of anode, middle and cathode increased to a certain extent with the increase of the electric field intensity when the low-voltage DC field with the intensity of 0~0.8 V/cm was applied, and it was significantly higher than that of the control group （CK）. The contents of chlorophyll a and chlorophyll b, antioxidant enzyme activities （CAT, POD, SOD）, plant height, plant stem, root length, fresh weight, aboveground biomass and underground biomass all showed a trend of low promotion and high inhibition with the increase of electric field intensity. When the electric field intensity was 0.2~0.4 V/cm, the parameters of Astragalus adsurgens Pall. in different areas were improved to different degrees. However, when the electric field intensity was 0.8 V/cm, the parameters of seedlings were inhibited. Therefore, the electric field intensity of 0.2~0.4 V/cmcan stimulate the growth of Astragalus adsurgens Pall. and may be the best range of electric field intensity to strengthen the process of plant restoration to a certain extent, while the electric field intensity of 0.8 V/cmwill cause stress on the physiology and root soil of Astragalus adsurgens Pall. and make it unable to grow well.

The effects of planting density on the growth and development of seedling of Polygonum cuspidatum Sieb.et Zucc.

LIU Zhi-pei, OUYANG You-xiang, ZHOU Jun, ZHOU Ming, ZHANG Ze-zhi

2021, 60(8): 107-109. doi:10.14088/j.cnki.issn0439-8114.2021.08.021

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The effects of different planting densities on the growth and development of annual Polygonum cuspidatum Sieb.et Zucc. seedlings were analyzed, and the growth and development of biennial seedlings under the planting density of 5 cm×5 cm. The results showed that different planting densities had an effect on the stem diameter of annual Polygonum cuspidatum Sieb.et Zucc. seedlings, but had no significant effect on the number of stem branches. Under the cultivation density of 5 cm×5 cm, biennial Polygonum cuspidatum Sieb.et Zucc. seed seedlings had more roots, longer taproots, taller stems on the ground, more bud heads, stem diameter≥0.74±0.15 mm, bud heads≥2. It may be that the planting density and years of Polygonum cuspidatum Sieb.et Zucc. seed seedlings are positively correlated with the underground rhizoma length and stem diameter growth of Polygonum cuspidatum Sieb.et Zucc. seedlings in the growth process, which needs further study.

Effects of combined application of plant growth hormone on ornamental traits of chrysanthemum

AO Di-xiu, CHEN Dong-liang, LIU Hua, LUO Chang, CHENG Xi, HUANG Cong-lin

2021, 60(8): 110-113. doi:10.14088/j.cnki.issn0439-8114.2021.08.022

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Fourteen ornamental chrysanthemums[Dendranthema morifolium （Ramat.） Tzvelev] sensitive to photoperiod were used to study the effects of different concentrations of daminozide and spraying time on their ornamental characters. The results show that different concentrations of daminozide had a greater effect on the plant height of the same variety, and the same concentration of daminozide had a smaller effect on the plant height of three types of chrysanthemum varieties with fast rooting, medium rooting and slow rooting. With the increase of concentration treated for every 7 days, the inhibition of plant height growth of chrysanthemum was more obvious. The plant height of chrysanthemum was better sprayed at the same concentration for every 10 days. For most varieties, 3 g/L daminozide was the best concentration to control the plant height once every 10 days. Different concentrations of daminozide had obvious effects on flower diameter, leaf length, leaf width, internode length and stem diameter of chrysanthemum varieties. Daminozide could inhibit plant height. The treatment of 3 g/L daminozide every 10 days had the best effect on the height of chrysanthemum.

Persistence evaluation of strontium marking on otoliths in larvae common carp （Cyprinus carpio）

QIU Chen, JIANG Tao, CHEN Xiu-bao, LIU Hong-bo, YANG Jian

2021, 60(8): 114-117. doi:10.14088/j.cnki.issn0439-8114.2021.08.023

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In order to investigate the persistence of Sr markings on otoliths, the larvae common carp （Cyprinus carpio） were marked in this study, using a single concentration of high Sr solution for 2 d immersion. A recognizable Sr marking region could be detected in the otolith, after a long period of recovery, using electron probe X ray micro chemical analysis（EPMA） technique. The results of mapping analysis of Sr content and the fluctuation of Sr/Ca concentration ratios along line transect from the core （0 μm） to the edge in otoliths showed that even after 607 d （i.e., 20 months） of recovery culture, a ring of red Sr marking region was still kept in the otolith. The Sr mark of otolith was firstly confirmed existence for quite a long time at least 20 months after exogenous Sr marking, suggesting that this method exactly have a strong stability, reliability, durability and application potential in the large-scale marking for fish proliferating and releasing and evaluation of corresponding effect.

Domestication and artificial reproduction of Chinese ink carp （Procypris mera）

XU Hua-jian, LU Zou-min, LIN Bin, LIU Qing-shan, TAN Dong-chu, WEI Xue-yao, TAN De-qing, HE Xing-heng, DING Yao, ZHANG Jian, HE Neng-zhen, WEI Xiang, HU Shi-gui

2021, 60(8): 118-120. doi:10.14088/j.cnki.issn0439-8114.2021.08.024

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The related technologies of broodstock domestication, artificial reproduction and seed cultivation of Chinese ink carp （Procypris mera） were tested. The results showed that after the fry were opened, they were cultivated in an outdoor cement pond by eutrophic water. After 30 days of cultivation, the fry with an average length of 6~10 mm reached 30~40 mm, and the survival rate was 68.7%. Since than, the fry began to switch to summerrlings cultivation stage.

The function of astragalus polysaccharides in hepatic steatosis of mice

WANG Chun-hua, SUN Xue-fang

2021, 60(8): 121-125. doi:10.14088/j.cnki.issn0439-8114.2021.08.025

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To study the preventive effect and mechanism of astragalus polysaccharides on diet induced hepatic steatosis of mice. The ICR mice were treated with high fat diet and 30% fructose water to building hepatic steatosis model. The mice of prevention groups were intragastric administration of 400 mg/kg astragalus polysaccharides at the same time of building model. After eight weeks, the liver histopathology, liver function, blood lipid and intestinal permeability were detected, and the expression of TLR4 and MyD88 protein in liver was analyzed by western blotting. Results showed that compared with the model group, astragalus polysaccharides caused the preventive group mice that the liver steatosis was reduced, the level of ALT, AST, ALP, TG, TC, GLU of serum were significantly reduced, the level of VDL was significantly increased, the intestinal permeability were decreased, and the expression level of TLR4 and MyD88 protein in liver was decreased. Astragalus polysaccharides can prevent hepatic steatosis, and its mechanism is related to improving intestinal barrier and regulating TLR4/MyD88 signaling pathway.

A the influence of feed prices on the price fluctuation of fresh milk in China—Based on VAR model

CUI Li-hang, GUO Rui

2021, 60(8): 126-130. doi:10.14088/j.cnki.issn0439-8114.2021.08.026

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In recent years, the price of fresh milk fluctuates frequently, which seriously affected the interests of producers and the health, stability and sustainable development of dairy industry. In this paper, monthly data of maize, soybean meal and fresh milk prices from January 2007 to December 2018 were selected as samples for empirical analysis. The fluctuation characteristics and changing trends of these three prices were analyzed. The VAR model wasused to explore the impact of feed prices on fresh milk prices, so as to reveal the impact mechanism of feed prices on fresh milk prices. The results showed that there wasa long-term stable equilibrium relationship between feed price and fresh milk price, and the change of feed price will inevitably lead to the change of fresh milk price, and the effect was lagging behind. In the short run, the rising feed prices represented by corn and soybean meal will inevitably lead to the rising price of fresh milk in the next period; in the long run, the price of fresh milk wasmainly affected by its own price, but the feed price will have a significant impact on the price of fresh milk.

Study on optimization of extraction process of volatile oil from mint produced in Anhui province

ZHAO Yu-qing, ZHANG Xin, MU Kai-lang

2021, 60(8): 131-133. doi:10.14088/j.cnki.issn0439-8114.2021.08.027

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The extraction technology of volatile oil of mint（Mentha canadensis Linnaeus） was optimized by steam distillation with the raw material of mint leaf produced in Huangshan, Anhui province. The effects of four extracting factors （solid-liquid ratio, distillation time, soaking time and ultrasonic time） were used. The results showed that the best extraction technology was the solid-liquid ratio 1∶11 （g∶mL）, distillation time 3.0 h, soaking time 2.0 h, and the ultrasonic time 30 min. Under the condition,the average extraction rate of volatile oil was 2.3%. The verification test also showed that the extraction process of volatile oil from mint was stable and reliable.

Study on process optimization for the extraction of pea protein and its emulsification and foaming activity

DAI Yuan, LENG Jin-song, LU Hong-jia

2021, 60(8): 134-139. doi:10.14088/j.cnki.issn0439-8114.2021.08.028

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Dry peas were used as research objects, which was ground into powder, and water was used as solvent to extract protein from pea flour. The extraction conditions were optimized using response surface analysis and the emulsification and foaming activity of the extracted products was determined. The results of the optimum extraction conditions within the scope of experimental research were as follows, the ratio of raw material to water 1∶30（g∶mL）, the type of enzyme alkali protease, extraction time 50 minutes, ultrasonic power 150 W. Under these conditions, the extraction rate of pea protein was up to 95.64%. The results of emulsification and foaming activity were as follows, emulsion activity 11.27 mL/g, emulsion stability 36.96 min, foaming activity 86.25%, foam stability 33.33%. Therefore, the optimization of pea protein extraction process technology was reasonable and feasible, and pea protein has stronger emulsification activity and emulsification stability, as well as stronger foaming activity and foam stability.

Study on the distribution of heavy metals in cattail in constructed wetlands

GE Guang-huan, KOU Kun, YU Su-hui, WANG Yang, WEN Zhi-liang, LI Xiang

2021, 60(8): 141-144. doi:10.14088/j.cnki.issn0439-8114.2021.08.029

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To explore the distribution of heavy metals in the cattails, the Zaohe constructed wetland and the Zaohe river constructed wetland demonstration base were selected as the research site. The cattails in the two artificial wetlands were taken as the research object, and the concentrations of copper and chromium in different parts of cattails were determined. The results showed that the cattail can absorb Cu well, and the content of heavy metals in cattail roots is the most, followed by the stem and leaves; The content of Cu is the highest in cattail, and the transfer coefficient of Cu in cattail is large, so the heavy metal in plants can be removed through seasonal harvesting; The cattails in two constructed wetlands can better remove the heavy metal Cu.

Establishment of the determination method of saikosaponin c,a,d in Radix bupleurum

LU Li-xiang, ZHOU Shi-you, ZENG Hai-sheng, ZHAO Feng-xian, YANG Gui-lin, TAN Yu-ping

2021, 60(8): 145-147. doi:10.14088/j.cnki.issn0439-8114.2021.08.030

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To establish a method for the determination of saikosaponins c, a, d in Bupleurum. using high-performance liquid chromatography, an Agilent SB-C18 column （4.6 mm×250 mm, 5 μm）, using acetonitrile-water as the mobile phase, gradient elution, flow rate 1.0 mL/min, detector for evaporative light Scatter detector. Results showed that the saikosaponins c, a, d were in the linear range of 2.028~20.280, 1.904~19.040, 2.112~21.120 μg, R² were 0.999 8, 0.999 6, 0.999 5, respectively.

Design of mobile system for agricultural tourism recommendation based on mixed user interest model

OU Dan

2021, 60(8): 148-152. doi:10.14088/j.cnki.issn0439-8114.2021.08.031

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In order to optimize and promote agricultural tourism information services and platform construction, a mixed user interest model based on short-term user interest and long-term user interest was established. Based on this, the core demand analysis and system module design analysis of the agricultural tourism system were carried out. Finally, the platform performance and user satisfaction of the mobile system were investigated. The results showed that user click-through rate and data visits were basically positively correlated, and the trend of the curve was basically the same. The mobile recommendation system can promptly feedback the user's operational needs and return the results. The satisfaction of tourists and farmers with the system has increased from 56% to 78%. At the same time, the satisfaction expectations of agricultural products and agricultural attractions recommendations have also increased from 77% and 68% to 96% and 95%, respectively.

Empirical analysis of the development of agricultural insurance and its influencing factors in Xinjiang

YUE Dong-qin, MA Qiong, HOU Yu-long, ZHOU Zi-wei

2021, 60(8): 153-156. doi:10.14088/j.cnki.issn0439-8114.2021.08.032

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Xinjiang is short of agricultural resources, frequent natural disasters and agricultural development is facing huge risks, the use of agricultural insurance to avoid the risks of agricultural development in Xinjiang plays an important role. By using Eviews 6.0 software to carry out multiple linear regression and establishing relevant models, this research studied the main factors that may affect the development of agricultural insurance in Xinjiang at this stage. The results showed that, the per capita net income of farmers and the loss ratio of agricultural insurance in the previous year had a positive impact on the premium income of agricultural insurance in Xinjiang, and the proportion of crop disaster area in disaster area had a negative impact on the premium income of agricultural insurance in Xinjiang. Finally, it put forward suggestions in order to stabilize the income of farmers and achieve social stability and long-term stability in Xinjiang.

Research on the quality evaluation of new urbanization development in Heilongjiang province based on entropy method

WANG Chong, ZHAI Xu-jun

2021, 60(8): 157-161. doi:10.14088/j.cnki.issn0439-8114.2021.08.033

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This research used entropy method to select 23 indexes from four dimensions to construct the evaluation index system of high-quality development of new urbanization in Heilongjiang province. The results showed that the overall development quality of new urbanization in Heilongjiang province showed a steady growth trend from 2008 to 2018, but the overall quality was not high. Among the four dimensions, the quality of residents' social life and the green ecological quality of human settlements were better, while the quality of economic development power and the coordinated development of urban and rural areas were relatively backward, which need to be adjusted. Finally, according to the evaluation results to make a detailed analysis and put forward corresponding suggestions, in order to provide a reference for the high-quality development of new urbanization in Heilongjiang province.

Intuitionistic fuzzy multi-attribute decision making based on improved Spearman rank correlation coefficient

GUO Min, TIAN Jia-xing

2021, 60(8): 162-170. doi:10.14088/j.cnki.issn0439-8114.2021.08.034

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A new decision method of Spearman rank correlation coefficient was proposed for multi-attribute decision making with intuitionistic fuzzy numbers. The attribute weights obtained by AHP and entropy weight method were used to construct the optimization model with the idea of game theory equilibrium, and the mixed penalty function was used to solve the optimal combination weights. The association degree of each attribute was calculated according to the idea of grey correlation analysis. The correlation degree and the optimal weight of each scheme were labeled according to their own size, which were taken into Spearman rank correlation coefficient calculation as comparison sequence and reference sequence respectively. The size of correlation coefficient obtained was used for priority arrangement. Finally, an example was given to verify the effectiveness of the method.

Study on the communication and development of rural science and technology information in Hubei province under the new media environment

FAN Qing, CHEN Hong-you

2021, 60(8): 171-175. doi:10.14088/j.cnki.issn0439-8114.2021.08.035

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In order to understand the application status and communication channels of rural science and technology information communication in Hubei province, this study conducted an investigation and research on the basis of analyzing the restrictive factors facing the development of rural science and technology information communication. According to the survey results, the current rural science and technology information communication in Hubei province had achieved some results under the rapid development of new media, but there were still some defects in the rural information communication environment, the construction of science and technology service platform, and the demand positioning of science and technology information, etc. Relevant suggestions were put forward to solve this problems.

Study on the benefit evaluation of meteorological disaster prevention and mitigation service of rainstorm disaster in Hunan province

DENG Ling, LI Xue-min, TAN Shan-shan, ZHANG Si-yuan, TAN Shi-qi

2021, 60(8): 176-179. doi:10.14088/j.cnki.issn0439-8114.2021.08.036

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Aiming at the meteorological disaster prevention and mitigation service of rainstorm disaster, the index of decision service benefit, public service benefit, social service benefit and inevitable loss factors were mainly considered, and the benefit evaluation model of meteorological disaster prevention and mitigation service of rainstorm disaster in Hunan province was established based on the backstepping method. The model was used to evaluate the meteorological disaster prevention and mitigation service benefit of six typical rainstorm weather processes in Hunan province from 2011 to 2015. The results showed that in recent years, the decision service benefit and social service benefit of rainstorm disaster in Hunan province were basically on the rise, and the public service benefit fluctuated greatly; the benefit of meteorological disaster prevention and mitigation service of rainstorm disaster accounted for 38.6%~57.0% of the direct economic loss of disaster, and the benefit of meteorological disaster prevention and mitigation service in a single rainstorm process was up to 1.366 billion yuan.

Construction and evaluation of circular economy efficiency evaluation index system：A case study of Shangluo city

DUAN Kai, WANG Ying

2021, 60(8): 180-184. doi:10.14088/j.cnki.issn0439-8114.2021.08.037

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By constructing the evaluation index system of Shangluo circular economy efficiency, the BCC model was used to evaluate the efficiency of circular economy in Shangluo city. The results showed that the scale efficiency of circular economy in Shangluo city was the best, but the pure technical efficiency was very low, which leaded to the input-output comprehensive efficiency is not ideal.

Influence of hierarchical diagnosis and treatment of medical institutions on rural residents' choice of medical treatment

YANG Qian, XIANG Li

2021, 60(8): 185-189. doi:10.14088/j.cnki.issn0439-8114.2021.08.038

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914 rural residents were selected by random stratified sampling method to understand their cognition of hierarchical diagnosis and treatment system, the characteristics of medical treatment choice and the relevant factors influencing their medical treatment choice. The results showed that, 57.33% of the respondents did not know the hierarchical diagnosis and treatment system, 79.87% of the respondents agreed with the hierarchical diagnosis and treatment system;when suffering from minor diseases/common diseases, 64.99% of the respondents chose to go to primary hospital, 20.02% of the respondents chose to go to secondary and tertiary hospital, and 14.99% of the respondents chose to go to private clinics / pharmacies;in terms of medical service attitude, hospital health environment, facilities and equipment perfection , the overall satisfaction degree of the respondents to the secondary and tertiary hospital was better than that of the primary hospital; average monthly income, distance from the nearest primary hospital（walking time）, awareness of hierarchical diagnosis and treatment system, purchase of medical insurance and self-assessment of health status were the main factors influencing the medical treatment choice of rural residents.

Research on the promotion mechanism and development strategy of waste classification for the construction of green civilization in new rural areas

LIU Xuan-wei

2021, 60(8): 190-194. doi:10.14088/j.cnki.issn0439-8114.2021.08.039

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The construction of green civilization is one of the important contents of building a new socialist countryside in an all-round way. Rural waste classification can not only improve the rural living environment, but also promote the construction of green civilization in new countryside. The promotion mechanism of rural waste classification to the construction of new rural green civilization was analyzed; through the establishment of SWOT model of rural waste classification, the advantages, disadvantages, opportunities and challenges of new rural waste classification to promote the construction of green civilization were analyzed, and the development strategies and specific measures of new rural waste classification were put forward.

Exploration and practice of rural revitalization pole-axis town and village mode：Taking Miaoba town in Dazhu county of Sichuan province as an example

ZHANG Yang, SHI Bin

2021, 60(8): 195-200. doi:10.14088/j.cnki.issn0439-8114.2021.08.040

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The revitalization strategy and method of town and village level is the key content of rural revitalization strategy. In the face of the fragmentation of rural construction, lack of growth points, lack of internal vitality, and difficulty in the continuation of characteristics, this study put forward four pole-axis application modes of spatial elements, cultural elements, rules and order, and elements composition based on the pole-axis system theory. Taking Miaoba town of Dazhu county in Sichuan province as an example, this study analyzed the current situation, characteristics and shortcomings of the pole-axis-cluster areas in rural areas, and formulated the development strategies and directions of various elements, so as to provide reference for the formulation of revitalization strategies and implementation plans at the town and village level.

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