黄淮麦区小麦品种（系）品质与抗性基因的KASP分型检测

doi:10.14088/j.cnki.issn0439-8114.2025.04.010

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (4): 51-57.doi: 10.14088/j.cnki.issn0439-8114.2025.04.010

黄淮麦区小麦品种（系）品质与抗性基因的KASP分型检测

王志¹, 李寒冰¹, 王伟¹, 张玉杰¹, 田蜜², 王伟伟¹

1.沧州市农林科学院/河北省旱碱麦重点实验室,河北沧州 061001;
2.辛集市农业技术推广中心,河北辛集 052300

收稿日期:2024-09-12 出版日期:2025-04-25 发布日期:2025-05-12
通讯作者: 王伟伟（1985-）,男,山东临沂人,副研究员,博士,主要从事植物抗逆遗传育种研究,（电子信箱）wangww1002@163.com。
作者简介:王志（1990-）,男,河北沧州人,助理研究员,硕士,主要从事小麦遗传育种研究,（电子信箱）w2707905@163.com。
基金资助:
河北省自然科学基金专项(C2023205050); 河北省耐盐碱作物种业科技创新团队项目（23327501D）; 国家现代农业产业技术体系项目(CARS-03)

Detection of quality and resistance genes of wheat varieties (lines) in Huang-Huai wheat region by KASP genotyping technology

WANG Zhi¹, LI Han-bing¹, WANG Wei¹, ZHANG Yu-jie¹, TIAN Mi², WANG Wei-wei¹

1. Cangzhou Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Drought-Alkali Tolerance in Wheat, Cangzhou 061001, Hebei, China;
2. Xinji Agricultural Technology Extension Center, Xinji 052300, Hebei, China

Received:2024-09-12 Published:2025-04-25 Online:2025-05-12

摘要/Abstract

摘要： 为解析黄淮麦区小麦（Triticum aestivum L.）主导品种的品质与抗性遗传基础,利用12个小麦品质、抗病、抗旱和抗穗发芽相关的KASP标记对该区99个小麦品种（系）进行检测。结果显示,品质方面,发现33份携带Glu-D1d基因编码的优质麦谷蛋白亚基5+10类型材料及39份含有Glu-A1基因编码的Ax1/Ax2*强筋单倍型材料;抗病性方面,仅12份具备Lr68叶锈病抗性基因,所有参试材料中均未检出抗赤霉病Fhb1基因;抗穗发芽检测中,TaSdr-B1a、Rio Blanco型及Hap-H抗穗发芽单倍型分别见于9份、73份及13份材料中;抗旱性方面,53份材料为COMT-3B基因的3Ba抗旱单倍型,45份为TaDREB-B1a抗旱单倍型,而89份材料为TaSST-4A基因的A2a抗旱单倍型。由此可知,黄淮麦区小麦品种中,所检测的抗旱基因分布广泛且占比较高,而抗病性方面优异单倍型占比较少,在育种策略上迫切需要强化抗病基因的引入与利用。

关键词: 小麦（Triticum aestivum L.）, 品质, 抗病, 抗旱, 抗穗发芽, 基因检测, KASP分型技术, 黄淮麦区

Abstract: To elucidate the genetic basis of quality and resistance traits in the dominant wheat （Triticum aestivum L.） varieties from the Huang-Huai wheat region, 99 wheat varieties (lines) were genotyped using 12 KASP markers related to quality, disease resistance, drought resistance and pre-harvest sprouting resistance. The results revealed that 33 materials carried the Glu-D1d gene encoding high-quality gluten subunit 5+10, and 39 materials contained the Glu-A1 gene encoding Ax1/Ax2* strong-gluten haplotype. In terms of disease resistance, only 12 materials exhibited Lr68, a leaf rust resistance gene, and none of the tested materials were found to carry the Fhb1 gene for Fusarium head blight resistantce. For sprout resistance, the haplotypes TaSdr-B1a, Rio Blanco type, and Hap-H associated with pre-harvest sprouting were detected in 9, 73, and 13 materials, respectively. In drought resistance, there were 53 materials carried 3Ba drought resistant haplotype of the COMT-3B gene, 45 materials possessed the TaDREB-B1a drought resistance haplotype, and 89 materials carried the A2a drought resistance haplotype of the TaSST-4A gene. It could be seen that the drought resistance genes detected in wheat varieties in Huang-Huai wheat region were widely distributed and accounted for a relatively high proportion, while the excellent haplotypes in disease resistance accounted for a relatively small proportion. Therefore, it was urgent to strengthen the introduction and utilization of disease resistance genes in breeding strategies.

Key words: wheat(Triticum aestivum L.), quality, disease resistance, drought resistance, pre-harvest sprouting resistance, gene detection, KASP genotyping technology, Huang-Huai wheat region

中图分类号:

S511
S336

王志, 李寒冰, 王伟, 张玉杰, 田蜜, 王伟伟. 黄淮麦区小麦品种（系）品质与抗性基因的KASP分型检测[J]. 湖北农业科学, 2025, 64(4): 51-57.

WANG Zhi, LI Han-bing, WANG Wei, ZHANG Yu-jie, TIAN Mi, WANG Wei-wei. Detection of quality and resistance genes of wheat varieties (lines) in Huang-Huai wheat region by KASP genotyping technology[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(4): 51-57.

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黄淮麦区小麦品种（系）品质与抗性基因的KASP分型检测

Detection of quality and resistance genes of wheat varieties (lines) in Huang-Huai wheat region by KASP genotyping technology

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