野生大麦EIL基因家族成员鉴定与表达分析

doi:10.14088/j.cnki.issn0439-8114.2025.08.034

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (8): 226-229.doi: 10.14088/j.cnki.issn0439-8114.2025.08.034

野生大麦EIL基因家族成员鉴定与表达分析

胡慧^1a, 门瑞龙^1bcd, 赵明卓^1bcd, 孙铭若^1bcd, 徐俊英^1bcd, 刘海洋^1bcd, 杨隆维^1bcd, 田雨², 余为仆³, 邱先进^1bcd

1.长江大学,a. 文理学院;b. 湿地生态与农业利用教育部工程研究中心;c.农业农村部长江中游作物绿色高效生产重点实验室（部省共建）;d.农学院,湖北荆州 434025;
2.宜昌市农业科学研究院,湖北宜昌 443004;
3.荆州农业科学院,湖北荆州 434000

收稿日期:2025-04-14 出版日期:2025-08-25 发布日期:2025-09-12
通讯作者: 邱先进（1984-）,湖北武汉人,教授,博士,主要从事作物分子遗传与育种研究工作,（电子信箱）xjqiu216@yangtzeu.edu.cn。
作者简介:胡慧（1985-）,女,湖北武汉人,讲师,主要从事作物分子遗传学研究工作,（电子信箱）240925404@qq.com ;共同第一作者,门瑞龙（2002-）,男,河南南阳人,在读硕士研究生,研究方向为作物遗传育种,（电子信箱）15037734101@163.com;
基金资助:
湿地生态与农业利用教育部工程研究中心开放基金项目（KFG202404）

Identification and expression analysis of members in the EIL gene family of wild barley

HU Hui^1a, MEN Rui-long^1bcd, ZHAO Ming-zhuo^1bcd, SUN Ming-ruo^1bcd, XU Jun-ying^1bcd, LIU Hai-yang^1bcd, YANG Long-wei^1bcd, TIAN Yu², YU Wei-pu³, QIU Xian-jin^1bcd

1a. College of Arts and Sciences; 1b. Engineering Research Center of Wetland Ecology and Agricultural Utilization, Ministry of Education; 1c. Key Laboratory of Green and Efficient Production of Crops in the Middle Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs (Co-constructed by Ministry and Province); 1d. College of Agriculture,Yangtze University,Jingzhou 434025, Hubei, China;
2. Yichang Academy of Agricultural Science, Yichang 443004, Hubei, China;
3. Jingzhou Academy of Agricultural Sciences, Jingzhou 434000, Hubei, China

Received:2025-04-14 Published:2025-08-25 Online:2025-09-12

摘要/Abstract

摘要： 基于全基因组分析技术对野生大麦（Hordeum spontaneum）EIL基因家族成员进行鉴定,解析其启动子区顺式作用元件的组成及分布特征,并分析其在逆境胁迫下的表达模式,挖掘潜在的抗逆调控基因。结果表明,野生大麦EIL基因家族包含7个成员,分别为HvEIL1、HvEIL2、HvEIL3、HvEIL4、HvEIL5、HvEIL6、HvEIL7。7个成员蛋白质长度平均为489 aa,分子质量均值为53 790.52 u,等电点为4.89~6.27,不稳定性系数为42.66~60.39,均为亲水性蛋白且定位于细胞核内。野生大麦EIL基因家族成员启动子区的顺式作用元件共有14种,主要包括光响应元件、植物激素响应元件和胁迫响应元件。在野生大麦转录组数据分析中,不同处理下基因的表达水平不同。HvEIL2、HvEIL3和HvEIL7在对照处理、干旱胁迫处理和干旱后复水处理中的表达量无明显差异,表明这些基因可能不参与干旱胁迫响应;HvEIL4的表达抑制可能与抗旱性增强相关,提示其可能对大麦抗旱性起负向调节作用。

关键词: 野生大麦（Hordeum spontaneum）, EIL基因家族成员, 鉴定, 表达分析

Abstract: Members of the EIL gene family in wild barley (Hordeum spontaneum) were identified based on whole-genome analysis technology; the composition and distribution characteristics of cis-acting elements in their promoter regions were analyzed; and their expression patterns under stress conditions were investigated to mine potential stress-resistant regulatory genes. The results showed that the EIL gene family in wild barley contained seven members, namely HvEIL1, HvEIL2, HvEIL3, HvEIL4, HvEIL5, HvEIL6, and HvEIL7. The seven members had an average protein length of 489 aa, a mean molecular weight of 53 790.52 u, isoelectric points ranging from 4.89 to 6.27, and instability indices between 42.66 and 60.39; all were hydrophilic proteins and localized in the nucleus. Cis-acting elements in the promoter regions of members in the EIL gene family of wild barley totaled 14 types, mainly including light-responsive elements, plant hormone-responsive elements, and stress-responsive elements. In transcriptome data analysis of wild barley, gene expression levels varied under different treatments. HvEIL2, HvEIL3, and HvEIL7 showed no significant differences in expression levels under control treatment, drought stress treatment, or rehydration treatment after drought, indicating that these genes might not participate in drought stress response. The suppression of HvEIL4 expression was associated with enhanced drought resistance, suggesting that it might play a negative regulatory role in barley drought tolerance.

Key words: wild barley (Hordeum spontaneum), members of the EIL gene family, identification, expression analysis

中图分类号:

S512

胡慧, 门瑞龙, 赵明卓, 孙铭若, 徐俊英, 刘海洋, 杨隆维, 田雨, 余为仆, 邱先进. 野生大麦EIL基因家族成员鉴定与表达分析[J]. 湖北农业科学, 2025, 64(8): 226-229.

HU Hui, MEN Rui-long, ZHAO Ming-zhuo, SUN Ming-ruo, XU Jun-ying, LIU Hai-yang, YANG Long-wei, TIAN Yu, YU Wei-pu, QIU Xian-jin. Identification and expression analysis of members in the EIL gene family of wild barley[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(8): 226-229.

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野生大麦EIL基因家族成员鉴定与表达分析

Identification and expression analysis of members in the EIL gene family of wild barley

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