Identification of different transcripts of porcine MMP14 in PAM cells and thier bioinformatics analysis

doi:10.14088/j.cnki.issn0439-8114.2020.23.038

Abstract

Abstract: Matrix metalloproteinases 14 (MMP14) is the first discovered membrane-type matrix metalloproteinases in the matrix metalloproteinases super family.MMP14 protein can hydrolyze various extracellular matrix components and serum proteins,promote extracellular matrix degradation,and hydrolyze cytokines,chemokines and growth factors.In this study,two different porcine MMP14 transcripts were found in pig alveolar macrophages (PAM).The expression levels of different transcripts of MMP14 gene in porcine PAM cells infected with PRRSV were detected by fluorescence quantitative PCR,the results showed that the expression level of transcript NM_214239.2 significantly reduced (P<0.05),while XM_021098303.1 significantly incresed (P<0.01) in PRRSV infected PAMs.Bioinformatics analysis results showed that these two different transcripts encoded two different proteins.Compared with NM_214239.2 encoded protein,the RRKR motif and PG_binding_1 functional region were missing in XM_021098303.1 encoded protein,which might not havefunctionalactivity in the inactivation of MMP14 protein.It is speculated that the inactivation of MMP14 after PRRSV infection maybe relate to the high expression of cytokines and chemokines stimulated by PRRSV.However,the specific role of MMP14 in PRRSV infection still needs further study.

Key words: pig, MMP14, different transcripts, PRRSV

CLC Number:

S828
S813

WU Jun-jing, WAN Xu-ling, HUANG Cang-yu, QIAO Mu, WU Hua-yu, MEI Shu-qi, PENG Xian-wen. Identification of different transcripts of porcine MMP14 in PAM cells and thier bioinformatics analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(23): 169-173.

References

[1] ZHANG Q Z,YOO D W.PRRS virus receptors and their role for pathogenesis[J].Veterinary microbiology,2015,177(3-4):229-241.
[2] SUZUKI A,LU J,KUSAKAI G,et al.ARK5 is a tumor invasion -associated factor downstream of Akt signaling[J].Molecular and cellular biology,2004,24(8):3526-3535.
[3] SEIKI M.Membrane-type 1 matrix metalloproteinase:A key enzyme for tumor invasion[J].Cancer letters,2003,194(1):1-11.
[4] GALVEZ B G,MATIAS-ROMAN S,ALBAR J P,et al.Membrane type 1-matrix metalloproteinase is activated during migration of human endothelial cells and modulates endothelial motility and matrix remodeling[J].Journal of biological chemistry,2001,276(40):37491-37500.
[5] GANGULY K,MAITY P,REITER R J,et al.Effect of melatonin on secreted and induced matrix metalloproteinase-9 and-2 activity during prevention of indomethacin-induced gastric ulcer[J].Journal of pineal research,2005,39(3):307-315.
[6] SWARNAKAR S,MISHRA A,GANGULY K,et al.Matrix metalloproteinase-9 activity and expression is reduced by melatonin during prevention of ethanol-induced gastric ulcer in mice[J].Journal of pineal research,2007,43(1):56-64.
[7] SATO H,TAKINO T,OKADA Y,et al.A matrix metalloproteinase expressed on the surface of invasive tumour cells[J].Nature,1994,370(6484):61-65.
[8] MORI H,TOMARI T,KOSHIKAWA N,et al.CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain[J].The EMBO Journal,2002,21(15):3949-3959.
[9] BRIERS Y,VOLCKAERT G,CORNELISSEN A,et al.Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages ϕKZ and EL[J].Molecular microbiology,2007,65(5):1334-1344.
[10] 陈国胜,谷欣,张想竹.细菌肽聚糖及其应用[J].安阳工学院学报,2007(6):36-38.
[11] ROY R,YANG J,MOSES M A.Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer[J].Journal of clinical oncology,2009,27(31):5287-5297.
[12] YANA I,WEISS S J.Regulation of membrane type-1 matrix metalloproteinase activation by proprotein convertases[J].Molecular biology of the cell,2000,11(7):2387-2401.
[13] PAHWA S,STAWIKOWSKI M J,FIELDS G B.Monitoring and inhibiting MT1-MMP during cancer initiation and progression[J].Cancers,2014,6(1):416-435.

[1]	ZHAO Hao-jian, ZHANG Huan-chun, LIU Ning, GUO Dui-tian, LI Shu-ping, ZHANG Li-li, ZOU Xiao-yu, YANG Jian-chao. Construction and operational strategy of order agricultural system based on blockchain technology——Taking Ningxiang pig in Ningxiang County, Changsha City as an example [J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(8): 202-206.
[2]	QIAO Tong-tong, ZHANG Yu, LI Zi-peng, QIAO Mu, QIN Zhen-yang, PENG Xian-wen, WU Jun-jing. Association analysis of NARS2 gene variation and carcass and meat quality traits in selenium black pig [J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 195-199.
[3]	LI Zheng, LIU Wen-chao, WANG Shi-yu. Research on operation mechanism and efficiency improvement of pig industry innovation ecosystem [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(9): 192-196.
[4]	WANG He-yun, SUN Tong, ZHOU Yi, JIN Hui, CHEN Jun-ge, YI Chun-long. The effects of light intensity and sedimental type on Chara braunii Gmel. in morphology, pigment pattern and sexual reproduction [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(8): 90-94.
[5]	WANG Yi-da, ZHOU Wang, CHEN Jin-jin, CAI Xiao-dong. Effects of different concentrations of NAA on cutting propagation of Caladium bicolosr under hydroponics [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(4): 81-84.
[6]	HE Wei-min, KUANG Shi-chang, LI Xiao-wen, WANG Chun, CUI Wei-tao, WANG Xi-liang, XIAO Yun-cai, LI Zi-li, ZHOU Zu-tao. Effects of microecological preparation of fermented Chinese medicine on the intestinal morphology, gene expression and flora of weaned piglets [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(16): 137-141.
[7]	DING Feng, LI Pan, TANG Ya-li, PU Sheng-hai. Application of integrated water and fertilizer technology of drip irrigation under film mulching in the production of pigment pepper [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 129-132.
[8]	YIN Peng, WANG Zhao-hua. Situation and trend of pig industry in Shandong province under the epidemic situation of African swine fever and New Coronavirus pneumonia [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(1): 133-138.
[9]	XU Xiao-wan, XIA Bi-bo, WU Zhi-ming, TIAN Yong-hong. Surveying DNA epigenetic diversity in the foreign hot pepper germplasms [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(6): 76-80.
[10]	HUANG Zi-xuan, XIONG Yan-zhen, MA Hui-qin, CHENG Jian-feng. Research progress on the influence of pigmented rice germplasm and environmental factors on its color [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 5-10.
[11]	LU Xing-hua, LIU Pei-hao, ZHANG Hao. Research on meat pigeon food quality control system based on the perspective of production chain [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 145-148.
[12]	ZHANG Li-ping, HUA Zai-dong, XIAO Hong-wei, REN Hong-yan, ZHU Zhe, BI Yan-zhen. Isolation, culture and identification of Hubei porcine spermatogonial stem cells [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(22): 128-132.
[13]	HE Wei-min, LI Xiao-wen, KUANG Shi-chang, WANG Chun, LI Kun, WANG Xi-liang, XIAO Yun-cai, LI Zi-li, ZHOU Zu-tao. ffects of fermented Chinese medicine microecological preparations on growth performance,serum biochemical indexes and antioxidant capacity of weaned piglets [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(18): 121-124.
[14]	ZHANG Wen-tao, GUO Yan-yu, HUANG Jin-hai, ZHANG Lei. Progress in research on inflammation and oxidative stress induced by PRRSV [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(16): 9-14.
[15]	QIAO Mu, LI Kang-chi, WU Jun-jing, WU Hua-yu, ZHOU Jia-wei, MEI Shu-qi, PENG Xian-wen. Polymorphism of porcine OLR1 gene and its association with liter size trait of Xidu black pigs [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(24): 141-143.